NIKR_BRUSU
ID NIKR_BRUSU Reviewed; 132 AA.
AC Q9AL83; G0KDH1;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Nickel-responsive regulator;
GN Name=nikR; OrderedLocusNames=BRA0805, BS1330_II0798;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=11133934; DOI=10.1128/jb.183.2.426-434.2001;
RA Jubier-Maurin V., Rodrigue A., Ouahrani-Bettache S., Layssac M.,
RA Mandrand-Berthelot M.-A., Koehler S., Liautard J.-P.;
RT "Identification of the nik gene cluster of Brucella suis: regulation and
RT contribution to urease activity.";
RL J. Bacteriol. 183:426-434(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between animal
RT and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/jb.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
CC -!- FUNCTION: Transcriptional repressor of the nikABCDE operon. Is active
CC in the presence of excessive concentrations of intracellular nickel (By
CC similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000250};
CC Note=Binds 1 nickel ion per subunit. {ECO:0000250};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC {ECO:0000305}.
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DR EMBL; AJ278644; CAC24692.1; -; Genomic_DNA.
DR EMBL; AE014292; AAN33983.1; -; Genomic_DNA.
DR EMBL; CP002998; AEM20259.1; -; Genomic_DNA.
DR RefSeq; WP_002965835.1; NZ_KN046805.1.
DR AlphaFoldDB; Q9AL83; -.
DR SMR; Q9AL83; -.
DR EnsemblBacteria; AEM20259; AEM20259; BS1330_II0798.
DR GeneID; 45126131; -.
DR GeneID; 55592454; -.
DR KEGG; bms:BRA0805; -.
DR KEGG; bsi:BS1330_II0798; -.
DR PATRIC; fig|204722.21.peg.3531; -.
DR HOGENOM; CLU_113319_1_4_5; -.
DR OMA; AHNCLET; -.
DR PhylomeDB; Q9AL83; -.
DR Proteomes; UP000007104; Chromosome II.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 3.30.70.1150; -; 1.
DR HAMAP; MF_00476; NikR; 1.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR002145; CopG.
DR InterPro; IPR022988; Ni_resp_reg_NikR.
DR InterPro; IPR014160; Nickel_NikR_proteobac.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR Pfam; PF08753; NikR_C; 1.
DR Pfam; PF01402; RHH_1; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR TIGRFAMs; TIGR02793; nikR; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nickel; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..132
FT /note="Nickel-responsive regulator"
FT /id="PRO_0000139284"
FT BINDING 77
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 88
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 96
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
SQ SEQUENCE 132 AA; 14930 MW; 953E68CF7E7F0E37 CRC64;
MQRITITIDD DLMAALDRMI EIKGYQNRSE ALRDLARTGL QQASLEEGQM EACVGVLSYT
YDHSARDLSK KLTNTHHDHH NISVASMHVH LDHDRCLEVS ILKGKTDDVR HFADHVKAER
HVTHGTLAVL PL
