NIKR_CITK8
ID NIKR_CITK8 Reviewed; 133 AA.
AC A8AR62;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Nickel-responsive regulator {ECO:0000255|HAMAP-Rule:MF_00476};
GN Name=nikR {ECO:0000255|HAMAP-Rule:MF_00476}; OrderedLocusNames=CKO_04931;
OS Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Citrobacter.
OX NCBI_TaxID=290338;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-895 / CDC 4225-83 / SGSC4696;
RG The Citrobacter koseri Genome Sequencing Project;
RA McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA Johnson M., Thiruvilangam P., Wilson R.;
RL Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcriptional repressor of the nikABCDE operon. Is active
CC in the presence of excessive concentrations of intracellular nickel.
CC {ECO:0000255|HAMAP-Rule:MF_00476}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00476};
CC Note=Binds 1 nickel ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00476};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC {ECO:0000255|HAMAP-Rule:MF_00476}.
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DR EMBL; CP000822; ABV15975.1; -; Genomic_DNA.
DR RefSeq; WP_012135616.1; NC_009792.1.
DR AlphaFoldDB; A8AR62; -.
DR SMR; A8AR62; -.
DR STRING; 290338.CKO_04931; -.
DR EnsemblBacteria; ABV15975; ABV15975; CKO_04931.
DR GeneID; 45138415; -.
DR KEGG; cko:CKO_04931; -.
DR HOGENOM; CLU_113319_1_4_6; -.
DR OMA; AHNCLET; -.
DR OrthoDB; 1885366at2; -.
DR Proteomes; UP000008148; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 3.30.70.1150; -; 1.
DR HAMAP; MF_00476; NikR; 1.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR002145; CopG.
DR InterPro; IPR022988; Ni_resp_reg_NikR.
DR InterPro; IPR014160; Nickel_NikR_proteobac.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR Pfam; PF08753; NikR_C; 1.
DR Pfam; PF01402; RHH_1; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR TIGRFAMs; TIGR02793; nikR; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nickel; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..133
FT /note="Nickel-responsive regulator"
FT /id="PRO_1000014060"
FT BINDING 76
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 87
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 89
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 95
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
SQ SEQUENCE 133 AA; 15079 MW; FFB3D657053E2424 CRC64;
MQRVTITLDD DLLETLDSLS QRRGYNNRSE AIRDILRGAL AQEATQEHGT QGFAVLSYVY
EHEKRDLASR IVSTQHHHHD LSVATLHVHI NHDDCLEIAV LKGDMGDVQH FADDVIAQRG
VRHGHLQCLP KEE
