NIKR_ECO57
ID NIKR_ECO57 Reviewed; 133 AA.
AC P0A6Z8; P28910; Q47559;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Nickel-responsive regulator;
GN Name=nikR; OrderedLocusNames=Z4873, ECs4348;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Transcriptional repressor of the nikABCDE operon. Is active
CC in the presence of excessive concentrations of intracellular nickel (By
CC similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000250};
CC Note=Binds 1 nickel ion per subunit. {ECO:0000250};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC {ECO:0000305}.
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DR EMBL; AE005174; AAG58608.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37771.1; -; Genomic_DNA.
DR PIR; D86018; D86018.
DR PIR; D91172; D91172.
DR RefSeq; NP_312375.1; NC_002695.1.
DR RefSeq; WP_001190062.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0A6Z8; -.
DR SMR; P0A6Z8; -.
DR STRING; 155864.EDL933_4712; -.
DR EnsemblBacteria; AAG58608; AAG58608; Z4873.
DR EnsemblBacteria; BAB37771; BAB37771; ECs_4348.
DR GeneID; 67417111; -.
DR GeneID; 915801; -.
DR KEGG; ece:Z4873; -.
DR KEGG; ecs:ECs_4348; -.
DR PATRIC; fig|386585.9.peg.4541; -.
DR eggNOG; COG0864; Bacteria.
DR HOGENOM; CLU_113319_1_4_6; -.
DR OMA; AHNCLET; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 3.30.70.1150; -; 1.
DR HAMAP; MF_00476; NikR; 1.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR002145; CopG.
DR InterPro; IPR022988; Ni_resp_reg_NikR.
DR InterPro; IPR014160; Nickel_NikR_proteobac.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR Pfam; PF08753; NikR_C; 1.
DR Pfam; PF01402; RHH_1; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR TIGRFAMs; TIGR02793; nikR; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nickel; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..133
FT /note="Nickel-responsive regulator"
FT /id="PRO_0000139275"
FT BINDING 76
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 87
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 89
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
FT BINDING 95
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250"
SQ SEQUENCE 133 AA; 15094 MW; 9A9E9F838D3FFEF1 CRC64;
MQRVTITLDD DLLETLDSLS QRRGYNNRSE AIRDILRSAL AQEATQQHGT QGFAVLSYVY
EHEKRDLASR IVSTQHHHHD LSVATLHVHI NHDDCLEIAV LKGDMGDVQH FADDVIAQRG
VRHGHLQCLP KED
