ID   NIKR_ECO5E              Reviewed;         133 AA.
AC   B5YUQ6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Nickel-responsive regulator {ECO:0000255|HAMAP-Rule:MF_00476};
GN   Name=nikR {ECO:0000255|HAMAP-Rule:MF_00476};
GN   OrderedLocusNames=ECH74115_4821;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Transcriptional repressor of the nikABCDE operon. Is active
CC       in the presence of excessive concentrations of intracellular nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_00476}.
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00476};
CC       Note=Binds 1 nickel ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00476};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC   -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC       {ECO:0000255|HAMAP-Rule:MF_00476}.
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DR   EMBL; CP001164; ACI36650.1; -; Genomic_DNA.
DR   RefSeq; WP_001190062.1; NC_011353.1.
DR   AlphaFoldDB; B5YUQ6; -.
DR   SMR; B5YUQ6; -.
DR   GeneID; 67417111; -.
DR   KEGG; ecf:ECH74115_4821; -.
DR   HOGENOM; CLU_113319_1_4_6; -.
DR   OMA; AHNCLET; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   Gene3D; 3.30.70.1150; -; 1.
DR   HAMAP; MF_00476; NikR; 1.
DR   InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR   InterPro; IPR045865; ACT-like_dom_sf.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR002145; CopG.
DR   InterPro; IPR022988; Ni_resp_reg_NikR.
DR   InterPro; IPR014160; Nickel_NikR_proteobac.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR   Pfam; PF08753; NikR_C; 1.
DR   Pfam; PF01402; RHH_1; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
DR   SUPFAM; SSF55021; SSF55021; 1.
DR   TIGRFAMs; TIGR02793; nikR; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nickel; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..133
FT                   /note="Nickel-responsive regulator"
FT                   /id="PRO_1000125817"
FT   BINDING         76
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   BINDING         87
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   BINDING         89
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT   BINDING         95
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
SQ   SEQUENCE   133 AA;  15094 MW;  9A9E9F838D3FFEF1 CRC64;
     MQRVTITLDD DLLETLDSLS QRRGYNNRSE AIRDILRSAL AQEATQQHGT QGFAVLSYVY
     EHEKRDLASR IVSTQHHHHD LSVATLHVHI NHDDCLEIAV LKGDMGDVQH FADDVIAQRG
     VRHGHLQCLP KED