NIKR_HELPG
ID NIKR_HELPG Reviewed; 148 AA.
AC B5Z8Y5;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Putative nickel-responsive regulator {ECO:0000255|HAMAP-Rule:MF_00476};
GN OrderedLocusNames=HPG27_1286;
OS Helicobacter pylori (strain G27).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=563041;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G27;
RX PubMed=18952803; DOI=10.1128/jb.01416-08;
RA Baltrus D.A., Amieva M.R., Covacci A., Lowe T.M., Merrell D.S.,
RA Ottemann K.M., Stein M., Salama N.R., Guillemin K.;
RT "The complete genome sequence of Helicobacter pylori strain G27.";
RL J. Bacteriol. 191:447-448(2009).
CC -!- FUNCTION: Transcriptional regulator. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00476};
CC Note=Binds 1 nickel ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00476};
CC -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC {ECO:0000255|HAMAP-Rule:MF_00476}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001173; ACI28034.1; -; Genomic_DNA.
DR RefSeq; WP_000380786.1; NC_011333.1.
DR PDB; 2Y3Y; X-ray; 2.39 A; A/B/C/D=58-148.
DR PDBsum; 2Y3Y; -.
DR AlphaFoldDB; B5Z8Y5; -.
DR BMRB; B5Z8Y5; -.
DR SMR; B5Z8Y5; -.
DR EnsemblBacteria; ACI28034; ACI28034; HPG27_1286.
DR KEGG; hpg:HPG27_1286; -.
DR HOGENOM; CLU_113319_1_2_7; -.
DR OMA; AHNCLET; -.
DR OrthoDB; 1885366at2; -.
DR Proteomes; UP000001735; Chromosome.
DR CollecTF; EXPREG_00000350; -.
DR GO; GO:0032993; C:protein-DNA complex; IPI:CollecTF.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IPI:CollecTF.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:CollecTF.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:CollecTF.
DR GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 3.30.70.1150; -; 1.
DR HAMAP; MF_00476; NikR; 1.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR002145; CopG.
DR InterPro; IPR022988; Ni_resp_reg_NikR.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR Pfam; PF08753; NikR_C; 1.
DR Pfam; PF01402; RHH_1; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Metal-binding; Nickel; Transcription;
KW Transcription regulation.
FT CHAIN 1..148
FT /note="Putative nickel-responsive regulator"
FT /id="PRO_1000125829"
FT BINDING 88
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 99
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 101
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 107
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT STRAND 63..72
FT /evidence="ECO:0007829|PDB:2Y3Y"
FT HELIX 79..89
FT /evidence="ECO:0007829|PDB:2Y3Y"
FT HELIX 90..93
FT /evidence="ECO:0007829|PDB:2Y3Y"
FT STRAND 94..103
FT /evidence="ECO:0007829|PDB:2Y3Y"
FT STRAND 106..116
FT /evidence="ECO:0007829|PDB:2Y3Y"
FT HELIX 117..129
FT /evidence="ECO:0007829|PDB:2Y3Y"
FT STRAND 133..140
FT /evidence="ECO:0007829|PDB:2Y3Y"
SQ SEQUENCE 148 AA; 17121 MW; 9C8A35303E7822B8 CRC64;
MDTPNKDDSI IRFSVSLQQN LLDELDNRII KNGYSSRSEL VRDMIREKLV EDNWAEDNPN
DESKIAVLVV IYDHHQRELN QRMIDIQHAS GTHVLCTTHI HMDEHNCLET IILQGNSFEI
QRLQLEIGGL RGVKFAKLTK ASSFEHNE
