NIKR_PYRHO
ID NIKR_PYRHO Reviewed; 138 AA.
AC O58316;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Putative nickel-responsive regulator {ECO:0000255|HAMAP-Rule:MF_00476};
GN OrderedLocusNames=PH0601;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Transcriptional regulator. {ECO:0000255|HAMAP-Rule:MF_00476}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00476};
CC Note=Binds 1 nickel ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00476};
CC -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC {ECO:0000255|HAMAP-Rule:MF_00476}.
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DR EMBL; BA000001; BAA29690.1; -; Genomic_DNA.
DR PIR; E71175; E71175.
DR RefSeq; WP_010884702.1; NC_000961.1.
DR PDB; 2BJ1; X-ray; 3.00 A; A/B=1-138.
DR PDB; 2BJ3; X-ray; 2.20 A; A/B/C/D=1-138.
DR PDB; 2BJ7; X-ray; 2.10 A; A/B=1-138.
DR PDB; 2BJ8; X-ray; 2.10 A; A/B=1-138.
DR PDB; 2BJ9; X-ray; 3.00 A; A/B=1-138.
DR PDBsum; 2BJ1; -.
DR PDBsum; 2BJ3; -.
DR PDBsum; 2BJ7; -.
DR PDBsum; 2BJ8; -.
DR PDBsum; 2BJ9; -.
DR AlphaFoldDB; O58316; -.
DR SMR; O58316; -.
DR STRING; 70601.3257007; -.
DR EnsemblBacteria; BAA29690; BAA29690; BAA29690.
DR GeneID; 1442936; -.
DR KEGG; pho:PH0601; -.
DR eggNOG; arCOG01008; Archaea.
DR OMA; AHNCLET; -.
DR OrthoDB; 90041at2157; -.
DR EvolutionaryTrace; O58316; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0010045; P:response to nickel cation; IEA:InterPro.
DR Gene3D; 1.10.1220.10; -; 1.
DR Gene3D; 3.30.70.1150; -; 1.
DR HAMAP; MF_00476; NikR; 1.
DR InterPro; IPR027271; Acetolactate_synth/TF_NikR_C.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR InterPro; IPR002145; CopG.
DR InterPro; IPR022988; Ni_resp_reg_NikR.
DR InterPro; IPR010985; Ribbon_hlx_hlx.
DR InterPro; IPR014864; TF_NikR_Ni-bd_C.
DR Pfam; PF08753; NikR_C; 1.
DR Pfam; PF01402; RHH_1; 1.
DR SUPFAM; SSF47598; SSF47598; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Metal-binding; Nickel; Transcription;
KW Transcription regulation.
FT CHAIN 1..138
FT /note="Putative nickel-responsive regulator"
FT /id="PRO_0000139312"
FT BINDING 78
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 89
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 91
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT BINDING 97
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00476"
FT STRAND 3..11
FT /evidence="ECO:0007829|PDB:2BJ7"
FT HELIX 12..25
FT /evidence="ECO:0007829|PDB:2BJ7"
FT HELIX 30..44
FT /evidence="ECO:0007829|PDB:2BJ7"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:2BJ3"
FT STRAND 51..63
FT /evidence="ECO:0007829|PDB:2BJ7"
FT HELIX 69..79
FT /evidence="ECO:0007829|PDB:2BJ7"
FT TURN 80..83
FT /evidence="ECO:0007829|PDB:2BJ7"
FT STRAND 84..91
FT /evidence="ECO:0007829|PDB:2BJ7"
FT STRAND 93..106
FT /evidence="ECO:0007829|PDB:2BJ7"
FT HELIX 107..118
FT /evidence="ECO:0007829|PDB:2BJ7"
FT STRAND 123..131
FT /evidence="ECO:0007829|PDB:2BJ7"
SQ SEQUENCE 138 AA; 15806 MW; A4B8715636C019FE CRC64;
MELIRFSISI PSKLLEKFDQ IIEEIGYENR SEAIRDLIRD FIIRHEWEVG NEEVAGTITI
VYNHDEGDVV KALLDLQHEY LDEIISSLHV HMDEHNCLEV IVVKGEAKKI KMIADKLLSL
KGVKHGKLVM TSTGKELV
