NIN3_ORYSJ
ID NIN3_ORYSJ Reviewed; 606 AA.
AC Q6H6N5; A0A0N7KFE8;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Neutral/alkaline invertase 3, chloroplastic {ECO:0000305};
DE Short=OsNIN3 {ECO:0000303|PubMed:17086397};
DE EC=3.2.1.26 {ECO:0000250|UniProtKB:Q10MC0};
DE Flags: Precursor;
GN Name=NIN3 {ECO:0000303|PubMed:17086397};
GN OrderedLocusNames=Os02g0529400 {ECO:0000312|EMBL:BAF08926.1},
GN LOC_Os02g32730 {ECO:0000305};
GN ORFNames=OsJ_06987 {ECO:0000312|EMBL:EAZ23290.1},
GN P0475F05.37-1 {ECO:0000312|EMBL:BAD25614.1},
GN P0476H10.21-1 {ECO:0000312|EMBL:BAD25431.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=17086397; DOI=10.1007/s00425-006-0430-x;
RA Murayama S., Handa H.;
RT "Genes for alkaline/neutral invertase in rice: alkaline/neutral invertases
RT are located in plant mitochondria and also in plastids.";
RL Planta 225:1193-1203(2007).
CC -!- FUNCTION: Mitochondrial invertase that cleaves sucrose into glucose and
CC fructose. {ECO:0000250|UniProtKB:Q10MC0}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000250|UniProtKB:Q10MC0};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17086397}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 100 family.
CC {ECO:0000305}.
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DR EMBL; AP004790; BAD25431.1; -; Genomic_DNA.
DR EMBL; AP004879; BAD25614.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08926.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79017.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ23290.1; -; Genomic_DNA.
DR EMBL; AK121301; BAH00419.1; -; mRNA.
DR RefSeq; XP_015627031.1; XM_015771545.1.
DR AlphaFoldDB; Q6H6N5; -.
DR SMR; Q6H6N5; -.
DR STRING; 4530.OS02T0529400-01; -.
DR CAZy; GH100; Glycoside Hydrolase Family 100.
DR PaxDb; Q6H6N5; -.
DR PRIDE; Q6H6N5; -.
DR EnsemblPlants; Os02t0529400-01; Os02t0529400-01; Os02g0529400.
DR GeneID; 4329537; -.
DR Gramene; Os02t0529400-01; Os02t0529400-01; Os02g0529400.
DR KEGG; osa:4329537; -.
DR eggNOG; ENOG502QPZ2; Eukaryota.
DR HOGENOM; CLU_020846_1_0_1; -.
DR InParanoid; Q6H6N5; -.
DR OMA; GQEWKII; -.
DR OrthoDB; 389322at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; Q6H6N5; baseline and differential.
DR Genevisible; Q6H6N5; OS.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0033926; F:glycopeptide alpha-N-acetylgalactosaminidase activity; IEA:InterPro.
DR GO; GO:0004575; F:sucrose alpha-glucosidase activity; IBA:GO_Central.
DR GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR024746; Glyco_hydro_100.
DR PANTHER; PTHR31916; PTHR31916; 1.
DR Pfam; PF12899; Glyco_hydro_100; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Chloroplast; Glycosidase; Hydrolase; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 59..606
FT /note="Neutral/alkaline invertase 3, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431506"
FT REGION 97..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 606 AA; 67683 MW; F3AA8ECCE5C9F8E4 CRC64;
MGIAEVALHS MPGAFAAHSP ASNLPLAADA ARGRRRRSAN SLHSSRALQG PVRFPGLRAA
VECQCQRIDD LARVTEGNGA WVKDAVDKAS HALGDVRVPG QAVGGNGSVN GSAAKPPPQR
RKASSVEDEA WELLRESVVY YCGSPVGTIA ANDPNDANPM NYDQVFIRDF IPSGIAFLLK
GEYEIVRNFI LHTLQLQSWE KTMDCHSPGQ GLMPASFKVR TIPLDGDEDA TEEVLDPDFG
EAAIGRVAPV DSGLWWIILL RAYGKCSGDL TVQERIDVQT GIKMILKLCL ADGFDMFPTL
LVTDGSCMID RRMGIHGHPL EIQALFYSAL LCAREMLTPE DGSADLIRAL NNRLIALSFH
IREYYWVDMQ KLNEIYRYKT EEYSYDAVNK FNIYPDQVSP WLVEWIPPKG GYFIGNLQPA
HMDFRFFSLG NLWSIVSSLA TTHQSHAILD LIESKWSDLV AEMPLKICYP ALENQEWKII
TGSDPKNTPW SYHNGGSWPT LLWQLTVASI KMNRPEIAAK AVEVAERRIA IDKWPEYYDT
KRARFIGKQS RLYQTWSIAG YLVAKQLLDK PDAARILSND EDSEILNALS TNRKRGKKVL
KKTFIV
