ID   NIN_BACSU               Reviewed;         132 AA.
AC   P12669;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=DNA-entry nuclease inhibitor;
DE   AltName: Full=Competence protein J;
GN   Name=nin; Synonyms=comJ; OrderedLocusNames=BSU03420;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2841296; DOI=10.1128/jb.170.8.3703-3710.1988;
RA   Vosman B., Kuiken G., Kooistra J., Venema G.;
RT   "Transformation in Bacillus subtilis: involvement of the 17-kilodalton DNA-
RT   entry nuclease and the competence-specific 18-kilodalton protein.";
RL   J. Bacteriol. 170:3703-3710(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7704255; DOI=10.1099/13500872-141-2-277;
RA   Fujishima Y., Yamane K.;
RT   "A 10 kb nucleotide sequence at the 5' flanking region (32 degrees) of
RT   srfAA of the Bacillus subtilis chromosome.";
RL   Microbiology 141:277-279(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
RX   PubMed=7746143; DOI=10.1111/j.1365-2958.1995.tb02236.x;
RA   van Sinderen D., Kiewiet R., Venema G.;
RT   "Differential expression of two closely related deoxyribonuclease genes,
RT   nucA and nucB, in Bacillus subtilis.";
RL   Mol. Microbiol. 15:213-223(1995).
CC   -!- FUNCTION: Plays a role in the competence of cells to be transformed. It
CC       inhibits the activity of the DNA-entry nuclease.
CC   -!- SUBUNIT: This protein is a subunit of a 75 kDa protein complex, which
CC       governs binding and entry of donor DNA. The complex is a tetramer of
CC       two subunits of the DNA-entry nuclease and two subunits of a
CC       competence-specific protein. Only the complex is able to bind ds- and
CC       ss-DNA.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
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DR   EMBL; M21672; AAA22413.1; -; Genomic_DNA.
DR   EMBL; D30762; BAA06430.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA08976.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12136.1; -; Genomic_DNA.
DR   PIR; B31100; B31100.
DR   RefSeq; NP_388224.1; NC_000964.3.
DR   RefSeq; WP_003246342.1; NZ_JNCM01000030.1.
DR   PDB; 4MQD; X-ray; 2.16 A; A/B/C/D=1-132.
DR   PDBsum; 4MQD; -.
DR   AlphaFoldDB; P12669; -.
DR   SMR; P12669; -.
DR   STRING; 224308.BSU03420; -.
DR   jPOST; P12669; -.
DR   PaxDb; P12669; -.
DR   PRIDE; P12669; -.
DR   DNASU; 938312; -.
DR   EnsemblBacteria; CAB12136; CAB12136; BSU_03420.
DR   GeneID; 938312; -.
DR   KEGG; bsu:BSU03420; -.
DR   PATRIC; fig|224308.179.peg.359; -.
DR   eggNOG; ENOG502ZSTE; Bacteria.
DR   OMA; DWTDEAI; -.
DR   BioCyc; BSUB:BSU03420-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.34.30; -; 1.
DR   InterPro; IPR038691; ComJ_sf.
DR   InterPro; IPR020354; Competence_nuclease_inhibitor.
DR   Pfam; PF11033; ComJ; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Competence; Membrane; Reference proteome.
FT   CHAIN           1..132
FT                   /note="DNA-entry nuclease inhibitor"
FT                   /id="PRO_0000096843"
FT   STRAND          3..5
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          8..11
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          16..21
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   HELIX           33..38
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          40..43
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          46..50
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          52..64
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          73..85
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          87..90
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          97..99
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          103..113
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   TURN            115..117
FT                   /evidence="ECO:0007829|PDB:4MQD"
FT   STRAND          124..131
FT                   /evidence="ECO:0007829|PDB:4MQD"
SQ   SEQUENCE   132 AA;  14997 MW;  B42840B732CD246B CRC64;
     MIKSWKPQEL SISYHQFTVF QKDSTPPVMD WTDEAIEKGY AAADGAISFE AQRNTKAFIL
     FRLNSSETVN SYEKKVTVPF HVTENGIHIE SIMSKRLSFD LPKGDYQLTC WTVPAEMSDL
     HADTYIIDAV SV