ID   NIP1_NICAL              Reviewed;         270 AA.
AC   P49173;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Probable aquaporin NIP-type;
DE   AltName: Full=Pollen-specific membrane integral protein;
OS   Nicotiana alata (Winged tobacco) (Persian tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pollen;
RA   Rodin J., Dodds P.N., McClure B.A., Newbigin E., Clarke A.E.;
RT   "Cloning and characterization of a pollen-specific protein from Nicotiana
RT   alata belonging to the MIP family of integral membrane proteins.";
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Aquaporins facilitate the transport of water and small
CC       neutral solutes across cell membranes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Pollen specific.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC
CC       1.A.8.12) subfamily. {ECO:0000305}.
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DR   EMBL; U20490; AAA62235.1; -; mRNA.
DR   AlphaFoldDB; P49173; -.
DR   SMR; P49173; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   CDD; cd00333; MIP; 1.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45724; PTHR45724; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..270
FT                   /note="Probable aquaporin NIP-type"
FT                   /id="PRO_0000064072"
FT   TRANSMEM        45..65
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        188..208
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        231..251
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   MOTIF           101..103
FT                   /note="NPA 1"
FT   MOTIF           213..215
FT                   /note="NPA 2"
SQ   SEQUENCE   270 AA;  28456 MW;  C8B72DB89F3B678F CRC64;
     MAKKDGNQEE EISQMEEGNI HSASNSDSNV GFCSSVSVVV ILQKLIAEAI GTYFVIFAGC
     GSVAVNKIYG SVTFPGICVT WGLIVMVMVY TVGYISGAHF NPAVTITFSI FGRFPWKQVP
     LYIIAQLMGS ILASGTLALL FDVTPQAYFG TVPVGSNGQS LAIEIIISFL LMFVISGVAT
     DDRAIGQVAG IAVGMTITLN VFVAGPISGA SMNPARSIGP AIVKHVYTGL WVYVVGPIIG
     TLAGAFVYNL IRSTDKPLRE LAKSASSLRS