NIP22_ORYSJ
ID NIP22_ORYSJ Reviewed; 298 AA.
AC Q67WJ8; B3ITC1;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Aquaporin NIP2-2 {ECO:0000305};
DE AltName: Full=Low silicon protein 6 {ECO:0000303|PubMed:18515498};
DE AltName: Full=NOD26-like intrinsic protein 2-2 {ECO:0000303|PubMed:16033806};
DE AltName: Full=OsNIP2;2 {ECO:0000303|PubMed:16033806};
GN Name=NIP2-2 {ECO:0000303|PubMed:16033806};
GN Synonyms=LSI6 {ECO:0000303|PubMed:18515498};
GN OrderedLocusNames=Os06g0228200 {ECO:0000312|EMBL:BAS96884.1},
GN LOC_Os06g12310 {ECO:0000305};
GN ORFNames=OsJ_019836 {ECO:0000312|EMBL:EAZ36353.1},
GN P0425F05.28-1 {ECO:0000312|EMBL:BAD37471.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18515498; DOI=10.1105/tpc.108.059311;
RA Yamaji N., Mitatni N., Ma J.F.;
RT "A transporter regulating silicon distribution in rice shoots.";
RL Plant Cell 20:1381-1389(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=16033806; DOI=10.1093/pcp/pci172;
RA Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M.;
RT "Identification of 33 rice aquaporin genes and analysis of their expression
RT and function.";
RL Plant Cell Physiol. 46:1568-1577(2005).
RN [8]
RP FUNCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=26283388; DOI=10.1073/pnas.1508987112;
RA Yamaji N., Sakurai G., Mitani-Ueno N., Ma J.F.;
RT "Orchestration of three transporters and distinct vascular structures in
RT node for intervascular transfer of silicon in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:11401-11406(2015).
CC -!- FUNCTION: Silicon transporter involved in the distribution of silicon
CC in shoots. Is responsible for the transport of silicon from the xylem
CC to the leaf tissues. Silicon is beneficial to plant growth and helps
CC plants to overcome abiotic and biotic stresses by preventing lodging
CC (falling over) and increasing resistance to pests and diseases, as well
CC as other stresses (PubMed:18515498). In the nodes, involved with LSI2
CC and LSI3 in silicon intervascular transfer, which is required for the
CC preferential distribution of silicon, such as hyperaccumulation of
CC silicon in the husk (PubMed:26283388). {ECO:0000269|PubMed:18515498,
CC ECO:0000269|PubMed:26283388}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18515498};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots and leaves
CC (PubMed:16033806). Expressed in roots, leaf sheaths and leaf blades. In
CC roots, it localizes in the plasma membrane of all cells of the young
CC region and is more abundant on the distal side than the proximal side
CC (at protein level). Expressed only in the xylem parenchyma cells
CC adjacent to vessels in both leaf sheaths and leaf blades (at protein
CC level). Is more abundant on the side facing the vessel (at protein
CC level) (PubMed:18515498). {ECO:0000269|PubMed:16033806,
CC ECO:0000269|PubMed:18515498}.
CC -!- INDUCTION: Down-regulated by silicon supply in roots and leaf blades.
CC {ECO:0000269|PubMed:18515498}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC
CC 1.A.8.12) subfamily. {ECO:0000305}.
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DR EMBL; AB253627; BAG54792.1; -; mRNA.
DR EMBL; AP003569; BAD37471.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19121.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS96884.1; -; Genomic_DNA.
DR EMBL; CM000143; EAZ36353.1; -; Genomic_DNA.
DR EMBL; AK072966; BAG93221.1; -; mRNA.
DR EMBL; AK112022; BAG99517.1; -; mRNA.
DR EMBL; AK104037; BAG96384.1; -; mRNA.
DR RefSeq; XP_015644134.1; XM_015788648.1.
DR AlphaFoldDB; Q67WJ8; -.
DR SMR; Q67WJ8; -.
DR STRING; 4530.OS06T0228200-01; -.
DR TCDB; 1.A.8.12.5; the major intrinsic protein (mip) family.
DR PaxDb; Q67WJ8; -.
DR PRIDE; Q67WJ8; -.
DR EnsemblPlants; Os06t0228200-01; Os06t0228200-01; Os06g0228200.
DR GeneID; 4340558; -.
DR Gramene; Os06t0228200-01; Os06t0228200-01; Os06g0228200.
DR KEGG; osa:4340558; -.
DR eggNOG; KOG0223; Eukaryota.
DR InParanoid; Q67WJ8; -.
DR OMA; YNLIRMT; -.
DR OrthoDB; 1152704at2759; -.
DR PlantReactome; R-OSA-9618218; Arsenic uptake and detoxification.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR ExpressionAtlas; Q67WJ8; baseline and differential.
DR Genevisible; Q67WJ8; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015267; F:channel activity; IEA:InterPro.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR45724; PTHR45724; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR PROSITE; PS00221; MIP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..298
FT /note="Aquaporin NIP2-2"
FT /id="PRO_0000286033"
FT TRANSMEM 54..74
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT MOTIF 111..113
FT /note="NPA 1"
FT MOTIF 222..224
FT /note="NPA 2"
SQ SEQUENCE 298 AA; 31827 MW; 83070442076AB26F CRC64;
MASTTAPSRT NSRVNYSNEI HDLSTVQSVS AVPSVYYPEK SFADIFPPNL LKKVISEVVA
TFLLVFVTCG AASIYGEDMK RISQLGQSVV GGLIVTVMIY ATGHISGAHM NPAVTLSFAF
FRHFPWIQVP FYWAAQFTGA MCAAFVLRAV LYPIEVLGTT TPTGPHWHAL VIEIVVTFNM
MFVTCAVATD SRAVGELAGL AVGSAVCITS IFAGPVSGGS MNPARTLAPA VASNVYTGLW
IYFLGPVVGT LSGAWVYTYI RFEEAPAAAG GAAPQKLSSF KLRRLQSQSM AADEFDNV
