ID   NIP32_ORYSJ             Reviewed;         305 AA.
AC   Q7EYH7; A0A0P0XCC1;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Aquaporin NIP3-2;
DE   AltName: Full=NOD26-like intrinsic protein 3-2;
DE   AltName: Full=OsNIP3;2;
GN   Name=NIP3-2; OrderedLocusNames=Os08g0152000, LOC_Os08g05590;
GN   ORFNames=OJ1349_D05.108, P0443G08.140;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [3]
RP   NOMENCLATURE, AND TISSUE SPECIFICITY.
RX   PubMed=16033806; DOI=10.1093/pcp/pci172;
RA   Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M.;
RT   "Identification of 33 rice aquaporin genes and analysis of their expression
RT   and function.";
RL   Plant Cell Physiol. 46:1568-1577(2005).
CC   -!- FUNCTION: Aquaporins facilitate the transport of water and small
CC       neutral solutes across cell membranes. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves and at lower levels in roots
CC       and anthers. {ECO:0000269|PubMed:16033806}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA).
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC
CC       1.A.8.12) subfamily. {ECO:0000305}.
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DR   EMBL; AP004461; BAC98554.1; -; Genomic_DNA.
DR   EMBL; AP005467; BAC99758.1; -; Genomic_DNA.
DR   EMBL; AP014964; BAT03848.1; -; Genomic_DNA.
DR   RefSeq; XP_015649638.1; XM_015794152.1.
DR   AlphaFoldDB; Q7EYH7; -.
DR   SMR; Q7EYH7; -.
DR   STRING; 4530.OS08T0152000-00; -.
DR   PaxDb; Q7EYH7; -.
DR   PRIDE; Q7EYH7; -.
DR   EnsemblPlants; Os08t0152000-01; Os08t0152000-01; Os08g0152000.
DR   GeneID; 107276042; -.
DR   Gramene; Os08t0152000-01; Os08t0152000-01; Os08g0152000.
DR   KEGG; osa:107276042; -.
DR   eggNOG; KOG0223; Eukaryota.
DR   HOGENOM; CLU_020019_3_1_1; -.
DR   InParanoid; Q7EYH7; -.
DR   OMA; MDEQHHG; -.
DR   OrthoDB; 1246320at2759; -.
DR   PlantReactome; R-OSA-9618218; Arsenic uptake and detoxification.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   Genevisible; Q7EYH7; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   PANTHER; PTHR45724; PTHR45724; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..305
FT                   /note="Aquaporin NIP3-2"
FT                   /id="PRO_0000286036"
FT   TRANSMEM        97..117
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   MOTIF           154..156
FT                   /note="NPA 1"
FT   MOTIF           265..267
FT                   /note="NPA 2"
SQ   SEQUENCE   305 AA;  31849 MW;  81C8F5811A5EFFD4 CRC64;
     MEGGKMSSMG MDAASASVTV PPMQMQAGDQ SNRIAIIISP RAGSSKILPF ELVNGAANAG
     SQRHADPAES TPEAHHHLWH PVDLPKIKPP VPLVKKVGAE FFGTFTLIFT VLSTIIMDEQ
     HKGVESLLGI ATSAGLAVTV LVLSLIHISG CHLNPAVSIA MTVFGHLPPA HLLPYIAAQI
     LGSITASFAV KGMYHPVNPG IVTVPKVGTV EAFFLEFVTT FVLLFIITAL ATDPNAVKEL
     IAVAVGATIM MNALVAGPST GASMNPARTL GPAIATGRYT QIWVYLVATP LGAVAGEGFY
     FAIKL