NIP61_ARATH
ID NIP61_ARATH Reviewed; 305 AA.
AC Q9SAI4; Q5PP68;
DT 27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Aquaporin NIP6-1;
DE AltName: Full=NOD26-like intrinsic protein 6-1;
DE Short=AtNIP6;1;
GN Name=NIP6-1; OrderedLocusNames=At1g80760; ORFNames=F23A5.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=11806824; DOI=10.1186/gb-2001-3-1-research0001;
RA Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J.;
RT "From genome to function: the Arabidopsis aquaporins.";
RL Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF ALA-119 AND VAL-252.
RX PubMed=16363796; DOI=10.1021/bi0511888;
RA Wallace I.S., Roberts D.M.;
RT "Distinct transport selectivity of two structural subclasses of the
RT nodulin-like intrinsic protein family of plant aquaglyceroporin channels.";
RL Biochemistry 44:16826-16834(2005).
CC -!- FUNCTION: Transports glycerol, urea and formamide, in Xenopus laevis
CC oocytes. Very low water transport activity.
CC {ECO:0000269|PubMed:16363796}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:11806824}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala/Val (NPA).
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC
CC 1.A.8.12) subfamily. {ECO:0000305}.
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DR EMBL; AC011713; AAF14664.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36445.1; -; Genomic_DNA.
DR EMBL; BT020229; AAV74223.1; -; mRNA.
DR EMBL; BT021924; AAX49373.1; -; mRNA.
DR PIR; B96840; B96840.
DR RefSeq; NP_178191.1; NM_106724.3.
DR AlphaFoldDB; Q9SAI4; -.
DR SMR; Q9SAI4; -.
DR BioGRID; 29633; 1.
DR IntAct; Q9SAI4; 1.
DR STRING; 3702.AT1G80760.1; -.
DR TCDB; 1.A.8.12.9; the major intrinsic protein (mip) family.
DR PaxDb; Q9SAI4; -.
DR PRIDE; Q9SAI4; -.
DR ProteomicsDB; 249436; -.
DR EnsemblPlants; AT1G80760.1; AT1G80760.1; AT1G80760.
DR GeneID; 844415; -.
DR Gramene; AT1G80760.1; AT1G80760.1; AT1G80760.
DR KEGG; ath:AT1G80760; -.
DR Araport; AT1G80760; -.
DR TAIR; locus:2025822; AT1G80760.
DR eggNOG; KOG0223; Eukaryota.
DR HOGENOM; CLU_020019_3_1_1; -.
DR InParanoid; Q9SAI4; -.
DR OMA; RRLIMYV; -.
DR OrthoDB; 1246320at2759; -.
DR PhylomeDB; Q9SAI4; -.
DR PRO; PR:Q9SAI4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SAI4; baseline and differential.
DR Genevisible; Q9SAI4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0046715; F:active borate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015267; F:channel activity; IEA:InterPro.
DR GO; GO:0015168; F:glycerol transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015204; F:urea transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0046713; P:borate transport; IMP:TAIR.
DR GO; GO:0080029; P:cellular response to boron-containing substance levels; IEP:TAIR.
DR CDD; cd00333; MIP; 1.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR034294; Aquaporin_transptr.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR PANTHER; PTHR45724; PTHR45724; 1.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR PROSITE; PS00221; MIP; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..305
FT /note="Aquaporin NIP6-1"
FT /id="PRO_0000064069"
FT TRANSMEM 82..102
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 139..141
FT /note="NPA 1"
FT MOTIF 250..252
FT /note="NPA 2"
FT MOD_RES 302
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P43286"
FT MUTAGEN 119
FT /note="A->W: 6-fold increase in water transport activity,
FT but impaired in urea transport."
FT /evidence="ECO:0000269|PubMed:16363796"
FT MUTAGEN 252
FT /note="V->A: No effect."
FT /evidence="ECO:0000269|PubMed:16363796"
SQ SEQUENCE 305 AA; 31845 MW; C6D6622660E5CDC8 CRC64;
MDHEEIPSTP STPATTPGTP GAPLFGGFEG KRNGHNGRYT PKSLLKSCKC FSVDNEWALE
DGRLPPVTCS LPPPNVSLYR KLGAEFVGTL ILIFAGTATA IVNQKTDGAE TLIGCAASAG
LAVMIVILST GHISGAHLNP AVTIAFAALK HFPWKHVPVY IGAQVMASVS AAFALKAVFE
PTMSGGVTVP TVGLSQAFAL EFIISFNLMF VVTAVATDTR AVGELAGIAV GATVMLNILI
AGPATSASMN PVRTLGPAIA ANNYRAIWVY LTAPILGALI GAGTYTIVKL PEEDEAPKER
RSFRR
