NIP7_MOUSE
ID NIP7_MOUSE Reviewed; 180 AA.
AC Q9CXK8; Q9D1B4;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=60S ribosome subunit biogenesis protein NIP7 homolog;
DE AltName: Full=Nucleolar pre-rRNA processing protein NIP7;
DE AltName: Full=PEachy;
DE AltName: Full=kDa93;
GN Name=Nip7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Embryonic head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for proper 34S pre-rRNA processing and 60S ribosome
CC subunit assembly. {ECO:0000250}.
CC -!- SUBUNIT: Monomer. Interacts with pre-ribosome complex. May bind to RNA.
CC Interacts with NOL8. Interacts with FTSJ3 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NIP7 family. {ECO:0000305}.
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DR EMBL; AK003739; BAB22972.1; -; mRNA.
DR EMBL; AK014290; BAB29247.1; -; mRNA.
DR EMBL; BC003972; AAH03972.1; -; mRNA.
DR CCDS; CCDS22644.1; -.
DR RefSeq; NP_079667.2; NM_025391.2.
DR AlphaFoldDB; Q9CXK8; -.
DR SMR; Q9CXK8; -.
DR BioGRID; 211262; 3.
DR IntAct; Q9CXK8; 1.
DR MINT; Q9CXK8; -.
DR STRING; 10090.ENSMUSP00000034392; -.
DR iPTMnet; Q9CXK8; -.
DR PhosphoSitePlus; Q9CXK8; -.
DR EPD; Q9CXK8; -.
DR MaxQB; Q9CXK8; -.
DR PaxDb; Q9CXK8; -.
DR PeptideAtlas; Q9CXK8; -.
DR PRIDE; Q9CXK8; -.
DR ProteomicsDB; 252895; -.
DR Antibodypedia; 16183; 235 antibodies from 31 providers.
DR DNASU; 66164; -.
DR Ensembl; ENSMUST00000034392; ENSMUSP00000034392; ENSMUSG00000031917.
DR GeneID; 66164; -.
DR KEGG; mmu:66164; -.
DR UCSC; uc009ngz.2; mouse.
DR CTD; 51388; -.
DR MGI; MGI:1913414; Nip7.
DR VEuPathDB; HostDB:ENSMUSG00000031917; -.
DR eggNOG; KOG3492; Eukaryota.
DR GeneTree; ENSGT00950000182971; -.
DR HOGENOM; CLU_097217_0_0_1; -.
DR InParanoid; Q9CXK8; -.
DR OMA; YKIWVKP; -.
DR OrthoDB; 1405073at2759; -.
DR PhylomeDB; Q9CXK8; -.
DR TreeFam; TF300081; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 66164; 29 hits in 72 CRISPR screens.
DR ChiTaRS; Nip7; mouse.
DR PRO; PR:Q9CXK8; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9CXK8; protein.
DR Bgee; ENSMUSG00000031917; Expressed in cleaving embryo and 271 other tissues.
DR ExpressionAtlas; Q9CXK8; baseline and differential.
DR Genevisible; Q9CXK8; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IBA:GO_Central.
DR GO; GO:0042255; P:ribosome assembly; IEA:InterPro.
DR Gene3D; 2.30.130.10; -; 1.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR016686; Ribosomal_synth_fac_NIP7.
DR InterPro; IPR040598; UPF0113_N.
DR InterPro; IPR005155; UPF0113_PUA.
DR Pfam; PF03657; UPF0113; 1.
DR Pfam; PF17833; UPF0113_N; 1.
DR PIRSF; PIRSF017190; Rbsml_synth_fac_NIP7; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ribosome biogenesis; RNA-binding.
FT CHAIN 1..180
FT /note="60S ribosome subunit biogenesis protein NIP7
FT homolog"
FT /id="PRO_0000218774"
FT DOMAIN 94..170
FT /note="PUA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT REGION 1..92
FT /note="N-terminal domain"
FT /evidence="ECO:0000250"
FT REGION 93..180
FT /note="C-terminal domain"
FT /evidence="ECO:0000250"
FT CONFLICT 10
FT /note="R -> P (in Ref. 1; BAB22972)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 180 AA; 20452 MW; 4F1256165370F2AF CRC64;
MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEM MLKLAANISG
DKLVSLGTCF GKFTKTHKFR LHVTALDYLA PYAKYKVWVK PGAEQSFLYG NHVLKSGLGR
ITENTSQYQG VVVYSMADIP LGFGVAAKST QDCRKVDPMA IVVFHQADIG EYVRHEETLT
