NIP7_YEAST
ID NIP7_YEAST Reviewed; 181 AA.
AC Q08962; D6W3F9;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=60S ribosome subunit biogenesis protein NIP7;
DE AltName: Full=Nuclear import protein 7;
GN Name=NIP7; OrderedLocusNames=YPL211W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP CHARACTERIZATION.
RX PubMed=9271378; DOI=10.1128/mcb.17.9.5001;
RA Zanchin N.I.T., Roberts P., DeSilva A., Sherman F., Goldfarb D.S.;
RT "Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome
RT subunit biogenesis.";
RL Mol. Cell. Biol. 17:5001-5015(1997).
RN [5]
RP CHARACTERIZATION, AND INTERACTION WITH NOP8 AND RRP43.
RX PubMed=9891085; DOI=10.1128/mcb.19.2.1518;
RA Zanchin N.I.T., Goldfarb D.S.;
RT "Nip7p interacts with Nop8p, an essential nucleolar protein required for
RT 60S ribosome biogenesis, and the exosome subunit Rrp43p.";
RL Mol. Cell. Biol. 19:1518-1525(1999).
RN [6]
RP INTERACTION WITH PRE-RIBOSOME COMPLEX.
RX PubMed=11583615; DOI=10.1016/s1097-2765(01)00342-2;
RA Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D.,
RA Lechner J., Hurt E.;
RT "Identification of a 60S preribosomal particle that is closely linked to
RT nuclear export.";
RL Mol. Cell 8:517-529(2001).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP RNA-BINDING, AND MUTAGENESIS OF 161-ILE--PHE-164.
RX PubMed=18001138; DOI=10.1021/bi7015876;
RA Coltri P.P., Guimaraes B.G., Granato D.C., Luz J.S., Teixeira E.C.,
RA Oliveira C.C., Zanchin N.I.;
RT "Structural insights into the interaction of the Nip7 PUA domain with
RT polyuridine RNA.";
RL Biochemistry 46:14177-14187(2007).
CC -!- FUNCTION: Required for proper 27S pre-rRNA processing and 60S ribosome
CC subunit assembly.
CC -!- SUBUNIT: Interacts with NOP8 and RRP43. Interacts with pre-ribosome
CC complex. May bind to RNA. {ECO:0000269|PubMed:11583615,
CC ECO:0000269|PubMed:9891085}.
CC -!- INTERACTION:
CC Q08962; Q08287: NOP8; NbExp=4; IntAct=EBI-12067, EBI-12135;
CC Q08962; P25359: RRP43; NbExp=5; IntAct=EBI-12067, EBI-1773;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleolus.
CC -!- MISCELLANEOUS: Present with 5170 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the NIP7 family. {ECO:0000305}.
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DR EMBL; Z73567; CAA97926.1; -; Genomic_DNA.
DR EMBL; AY558059; AAS56385.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11225.1; -; Genomic_DNA.
DR PIR; S65230; S65230.
DR RefSeq; NP_015113.1; NM_001184025.1.
DR PDB; 6ELZ; EM; 3.30 A; l=1-181.
DR PDB; 6EM5; EM; 4.30 A; l=1-181.
DR PDB; 7OHR; EM; 4.72 A; l=1-181.
DR PDBsum; 6ELZ; -.
DR PDBsum; 6EM5; -.
DR PDBsum; 7OHR; -.
DR AlphaFoldDB; Q08962; -.
DR SMR; Q08962; -.
DR BioGRID; 35974; 356.
DR DIP; DIP-1456N; -.
DR IntAct; Q08962; 48.
DR MINT; Q08962; -.
DR STRING; 4932.YPL211W; -.
DR iPTMnet; Q08962; -.
DR MaxQB; Q08962; -.
DR PaxDb; Q08962; -.
DR PRIDE; Q08962; -.
DR EnsemblFungi; YPL211W_mRNA; YPL211W; YPL211W.
DR GeneID; 855890; -.
DR KEGG; sce:YPL211W; -.
DR SGD; S000006132; NIP7.
DR VEuPathDB; FungiDB:YPL211W; -.
DR eggNOG; KOG3492; Eukaryota.
DR GeneTree; ENSGT00950000182971; -.
DR HOGENOM; CLU_097217_0_0_1; -.
DR InParanoid; Q08962; -.
DR OMA; YKIWVKP; -.
DR BioCyc; YEAST:G3O-34102-MON; -.
DR PRO; PR:Q08962; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q08962; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005730; C:nucleolus; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:1902626; P:assembly of large subunit precursor of preribosome; IMP:SGD.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
DR GO; GO:0042255; P:ribosome assembly; IEA:InterPro.
DR Gene3D; 2.30.130.10; -; 1.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR016686; Ribosomal_synth_fac_NIP7.
DR InterPro; IPR040598; UPF0113_N.
DR InterPro; IPR005155; UPF0113_PUA.
DR Pfam; PF03657; UPF0113; 1.
DR Pfam; PF17833; UPF0113_N; 1.
DR PIRSF; PIRSF017190; Rbsml_synth_fac_NIP7; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Reference proteome; Ribosome biogenesis;
KW RNA-binding.
FT CHAIN 1..181
FT /note="60S ribosome subunit biogenesis protein NIP7"
FT /id="PRO_0000218776"
FT DOMAIN 94..170
FT /note="PUA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT MUTAGEN 161..164
FT /note="IVAF->AVAA: Reduces RNA-binding ability in vitro."
FT /evidence="ECO:0000269|PubMed:18001138"
SQ SEQUENCE 181 AA; 20381 MW; 82EAE8E4B2FCC31A CRC64;
MRQLTEEETK VVFEKLAGYI GRNISFLVDN KELPHVFRLQ KDRVYYVPDH VAKLATSVAR
PNLMSLGICL GKFTKTGKFR LHITSLTVLA KHAKYKIWIK PNGEMPFLYG NHVLKAHVGK
MSDDIPEHAG VIVFAMNDVP LGFGVSAKST SESRNMQPTG IVAFRQADIG EYLRDEDTLF
T
