NIPA1_ARATH
ID NIPA1_ARATH Reviewed; 335 AA.
AC Q9LIR9;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable magnesium transporter NIPA1;
GN OrderedLocusNames=At3g23870; ORFNames=F14O13.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but
CC to a much less extent than Mg(2+) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Early endosome {ECO:0000250}. Note=Recruited to
CC the cell membrane in response to low extracellular magnesium.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NIPA (TC 2.A.7) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX824470; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AP001297; BAB03004.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76824.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64589.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64591.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64592.1; -; Genomic_DNA.
DR EMBL; BX824470; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001326605.1; NM_001338660.1.
DR RefSeq; NP_001326607.1; NM_001338658.1.
DR RefSeq; NP_001326608.1; NM_001338659.1.
DR RefSeq; NP_189029.1; NM_113292.2.
DR AlphaFoldDB; Q9LIR9; -.
DR BioGRID; 7302; 1.
DR IntAct; Q9LIR9; 1.
DR STRING; 3702.AT3G23870.1; -.
DR TCDB; 2.A.7.25.5; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q9LIR9; -.
DR PRIDE; Q9LIR9; -.
DR EnsemblPlants; AT3G23870.1; AT3G23870.1; AT3G23870.
DR EnsemblPlants; AT3G23870.2; AT3G23870.2; AT3G23870.
DR EnsemblPlants; AT3G23870.3; AT3G23870.3; AT3G23870.
DR EnsemblPlants; AT3G23870.4; AT3G23870.4; AT3G23870.
DR GeneID; 821970; -.
DR Gramene; AT3G23870.1; AT3G23870.1; AT3G23870.
DR Gramene; AT3G23870.2; AT3G23870.2; AT3G23870.
DR Gramene; AT3G23870.3; AT3G23870.3; AT3G23870.
DR Gramene; AT3G23870.4; AT3G23870.4; AT3G23870.
DR KEGG; ath:AT3G23870; -.
DR Araport; AT3G23870; -.
DR TAIR; locus:2076186; AT3G23870.
DR eggNOG; KOG2922; Eukaryota.
DR HOGENOM; CLU_012349_1_1_1; -.
DR InParanoid; Q9LIR9; -.
DR OMA; YGQTHMI; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; Q9LIR9; -.
DR PRO; PR:Q9LIR9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LIR9; baseline and differential.
DR Genevisible; Q9LIR9; AT.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..335
FT /note="Probable magnesium transporter NIPA1"
FT /id="PRO_0000430289"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..79
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..143
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..335
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 303..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 315
FT /note="D -> E (in Ref. 3; BX824470)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 36292 MW; B5443E430824BF43 CRC64;
MDQMSPDNIN GVILAVSSSI FIGSSFIIKK KGLKKAGASG VRAGEGGYGY LKEPWWWAGM
ITMIVGEVAN FAAYAFAPAI LVTPLGALSI IFSAVLAHFI LKEKLHMFGI LGCILCVVGS
TTIVLHAPHE QKIESVKQIW QLAIEPGFLV YSAVIVIVVA ILIFYYEPRY GKTHMIVYVG
ICSLMGSLTV MSVKAVAIAI KLTFSGTNQF KYFNTWIFIL VVATCCILQI NYLNKALDTF
NTAVISPVYY VMFTTFTIIA SMIMFKDWAS QSGLKIATEL CGFVTILSGT FLLHKTKDMG
NSASGRGSIS MPTRDTPVFT NSGSGRSSSS DKVAS
