NIPA2_ARATH
ID NIPA2_ARATH Reviewed; 336 AA.
AC B3LFA3; Q8LA45; Q9SVN3;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Probable magnesium transporter NIPA2;
GN OrderedLocusNames=At4g13800; ORFNames=F18A5.190;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-336.
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but
CC to a much less extent than Mg(2+) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Early endosome {ECO:0000250}. Note=Recruited to
CC the cell membrane in response to low extracellular magnesium.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NIPA (TC 2.A.7) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36844.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78422.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035528; CAB36844.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161537; CAB78422.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83326.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66023.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66025.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66026.1; -; Genomic_DNA.
DR EMBL; BT033091; ACF04814.1; -; mRNA.
DR EMBL; AY088039; AAM65585.1; -; mRNA.
DR PIR; T05249; T05249.
DR RefSeq; NP_001319923.1; NM_001340877.1.
DR RefSeq; NP_001327951.1; NM_001340884.1.
DR RefSeq; NP_001327952.1; NM_001340881.1.
DR RefSeq; NP_567411.1; NM_117454.2.
DR AlphaFoldDB; B3LFA3; -.
DR STRING; 3702.AT4G13800.1; -.
DR iPTMnet; B3LFA3; -.
DR PaxDb; B3LFA3; -.
DR PRIDE; B3LFA3; -.
DR ProteomicsDB; 249439; -.
DR EnsemblPlants; AT4G13800.1; AT4G13800.1; AT4G13800.
DR EnsemblPlants; AT4G13800.10; AT4G13800.10; AT4G13800.
DR EnsemblPlants; AT4G13800.5; AT4G13800.5; AT4G13800.
DR EnsemblPlants; AT4G13800.8; AT4G13800.8; AT4G13800.
DR GeneID; 827014; -.
DR Gramene; AT4G13800.1; AT4G13800.1; AT4G13800.
DR Gramene; AT4G13800.10; AT4G13800.10; AT4G13800.
DR Gramene; AT4G13800.5; AT4G13800.5; AT4G13800.
DR Gramene; AT4G13800.8; AT4G13800.8; AT4G13800.
DR KEGG; ath:AT4G13800; -.
DR Araport; AT4G13800; -.
DR TAIR; locus:2119540; AT4G13800.
DR eggNOG; KOG2922; Eukaryota.
DR HOGENOM; CLU_012349_1_1_1; -.
DR InParanoid; B3LFA3; -.
DR OMA; MGAGEVC; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; B3LFA3; -.
DR PRO; PR:B3LFA3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; B3LFA3; baseline and differential.
DR Genevisible; B3LFA3; AT.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..336
FT /note="Probable magnesium transporter NIPA2"
FT /id="PRO_0000430290"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..79
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..145
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..336
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 303..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 329
FT /note="N -> H (in Ref. 4; AAM65585)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 336 AA; 36600 MW; A07AE7BBAF84B5FA CRC64;
MEEMSPDNIH GVILAVSSSI FIGSSFIIKK KGLKKAGVSG ARAGEGGYGY LYEPWWWAGM
ITMIVGEIAN FAAYAFAPAI LVTPLGALSI IFSAVLAHFI LEEKLHMFGI LGCVLCVVGS
TTIVLHAPHE QGIESVKQVW HLATEPGFLA YSAVVLVVVL ALIFYYEPRY GKTHMIVYVG
ICSLMGSLTV MSVKAVAIAI KLTFSGMNQF KYFHAWIFII VVTICCILQI NYLNKALDNF
NTAVISPVYY VMFTTFTILA SMIMFKDWAS QSGLQIATEL CGFVTILSGT FLLHKTKDMG
NSTSLRGSTS HSPRDTPVFI NSGSSRSSNS TRPAIL
