NIPA3_ARATH
ID NIPA3_ARATH Reviewed; 368 AA.
AC Q9LNK7;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable magnesium transporter NIPA3;
GN OrderedLocusNames=At1g34470; ORFNames=F12K21.21, F7P12.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but
CC to a much less extent than Mg(2+) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Early endosome {ECO:0000250}. Note=Recruited to
CC the cell membrane in response to low extracellular magnesium.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NIPA (TC 2.A.7) family. {ECO:0000305}.
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DR EMBL; AC023279; AAF79266.1; -; Genomic_DNA.
DR EMBL; AC023913; AAG51905.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31717.1; -; Genomic_DNA.
DR EMBL; BT024742; ABD59080.1; -; mRNA.
DR RefSeq; NP_564447.1; NM_103169.3.
DR AlphaFoldDB; Q9LNK7; -.
DR BioGRID; 25581; 32.
DR IntAct; Q9LNK7; 31.
DR STRING; 3702.AT1G34470.1; -.
DR iPTMnet; Q9LNK7; -.
DR PaxDb; Q9LNK7; -.
DR PRIDE; Q9LNK7; -.
DR ProteomicsDB; 251113; -.
DR EnsemblPlants; AT1G34470.1; AT1G34470.1; AT1G34470.
DR GeneID; 840349; -.
DR Gramene; AT1G34470.1; AT1G34470.1; AT1G34470.
DR KEGG; ath:AT1G34470; -.
DR Araport; AT1G34470; -.
DR TAIR; locus:2009343; AT1G34470.
DR eggNOG; KOG2922; Eukaryota.
DR HOGENOM; CLU_012349_1_1_1; -.
DR InParanoid; Q9LNK7; -.
DR OMA; EPGFLCY; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; Q9LNK7; -.
DR PRO; PR:Q9LNK7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LNK7; baseline and differential.
DR Genevisible; Q9LNK7; AT.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..368
FT /note="Probable magnesium transporter NIPA3"
FT /id="PRO_0000430291"
FT TOPO_DOM 1..18
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..66
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..90
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..115
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 137..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..220
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..255
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..368
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 368 AA; 39797 MW; 84E9CD9C59FCF162 CRC64;
MASLSGSWRD AYKGMSSDNI KGLVLALSSS LFIGASFIVK KKGLKRAGAS GLRAGSGGYS
YLLEPLWWVG MITMIVGEIA NFAAYAFAPA ILVTPLGALS IIISAALAHV ILHEKLHTFG
LLGCVLCVVG SITIVLHAPQ EQEIDSVLQV WNLATEPAFL LYAAAVVGAA IILIVQFVPQ
YGQSHVMVYI GVCSLVGSLS VMSVKALGIA LKLTFSGMNQ LIYPQTWVFT LIVLTCVITQ
MNYLNKALDT FNTAVVSPIY YVMFTSLTIL ASVIMFKDWD RQDGTQIVTE LCGFVTILSG
TFLLHKTKDM VDGSSSLGNL ALRLPKQLED SNGFEQEGIP LTLRRHECTK SPRPMRQFIL
PQDGPEAV
