NIPA3_MOUSE
ID NIPA3_MOUSE Reviewed; 416 AA.
AC Q8BMW7;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Magnesium transporter NIPA3;
DE AltName: Full=NIPA-like protein 1;
DE AltName: Full=Non-imprinted in Prader-Willi/Angelman syndrome region protein 3 homolog;
GN Name=Nipal1; Synonyms=Nipa3, Npal1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Extraembryonic tissue, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP FUNCTION, INDUCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=18667602; DOI=10.1152/ajpcell.00091.2008;
RA Goytain A., Hines R.M., Quamme G.A.;
RT "Functional characterization of NIPA2, a selective Mg2+ transporter.";
RL Am. J. Physiol. 295:C944-C953(2008).
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+), Cu(2+) and
CC Co(2+) but to a much less extent than Mg(2+).
CC {ECO:0000269|PubMed:18667602}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.90 mM for magnesium ions {ECO:0000269|PubMed:18667602};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Up-regulated by low magnesium ion levels.
CC {ECO:0000269|PubMed:18667602}.
CC -!- SIMILARITY: Belongs to the NIPA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25435.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK014427; BAC25435.1; ALT_FRAME; mRNA.
DR EMBL; AC122733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS39111.1; -.
DR RefSeq; NP_001074674.1; NM_001081205.1.
DR AlphaFoldDB; Q8BMW7; -.
DR STRING; 10090.ENSMUSP00000084462; -.
DR TCDB; 2.A.7.25.9; the drug/metabolite transporter (dmt) superfamily.
DR GlyGen; Q8BMW7; 4 sites.
DR PhosphoSitePlus; Q8BMW7; -.
DR PaxDb; Q8BMW7; -.
DR PRIDE; Q8BMW7; -.
DR ProteomicsDB; 253068; -.
DR Antibodypedia; 52126; 67 antibodies from 14 providers.
DR Ensembl; ENSMUST00000087212; ENSMUSP00000084462; ENSMUSG00000067219.
DR GeneID; 70701; -.
DR KEGG; mmu:70701; -.
DR UCSC; uc008xrv.1; mouse.
DR CTD; 152519; -.
DR MGI; MGI:1917951; Nipal1.
DR VEuPathDB; HostDB:ENSMUSG00000067219; -.
DR eggNOG; KOG2922; Eukaryota.
DR GeneTree; ENSGT00940000160022; -.
DR HOGENOM; CLU_012349_1_3_1; -.
DR InParanoid; Q8BMW7; -.
DR OMA; MSCKGLG; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; Q8BMW7; -.
DR TreeFam; TF313214; -.
DR Reactome; R-MMU-5223345; Miscellaneous transport and binding events.
DR BioGRID-ORCS; 70701; 3 hits in 70 CRISPR screens.
DR PRO; PR:Q8BMW7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BMW7; protein.
DR Bgee; ENSMUSG00000067219; Expressed in gastrula and 91 other tissues.
DR ExpressionAtlas; Q8BMW7; baseline and differential.
DR Genevisible; Q8BMW7; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; IDA:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Magnesium; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..416
FT /note="Magnesium transporter NIPA3"
FT /id="PRO_0000386645"
FT TOPO_DOM 1..67
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..165
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..207
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..273
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..336
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 416 AA; 45159 MW; 3BCEBB63897DEF65 CRC64;
MGVQLRLPPG EPCHEGYVLS LVCSNSSRAS CEITNVSESL PYPVVYTNLN SSKTNFSISA
SVENKYNLYV GLVLAISSSV FIGSSFILKK KGLLQLADKG ITRAGQGGHS YLKEWLWWAG
LLSMGAGEAA NFAAYAFAPA TLVTPLGALS VLISAILSSY FLNERLNIHG KIGCILSVLG
STVMVIHAPQ EGEVTSLHEM EMKLRDPGFV SFAVIISVIS LVLILIVAPK KGQTNILVYI
AICSLIGAFS VSSVKGLGIA IKELLERKPV YKDPLFFILL TMLALSVTTQ INYLNKALDT
FNTSLVTPIY YVFFTSMVVT CSAILFQEWY GMKAGDIIGT LSGFFTIING IFLLHAFKNT
NITWSELMST AKKEALSPNG NQNSYVLLEN ADFSASGYDD DITLFSRTND QSSHKL
