NIPA4_MOUSE
ID NIPA4_MOUSE Reviewed; 406 AA.
AC Q8BZF2; A4QPF8;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Magnesium transporter NIPA4;
DE AltName: Full=Ichthyin;
DE AltName: Full=NIPA-like protein 4;
DE AltName: Full=Non-imprinted in Prader-Willi/Angelman syndrome region protein 4 homolog;
GN Name=Nipal4; Synonyms=Ichn, Nipa4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, INDUCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=18667602; DOI=10.1152/ajpcell.00091.2008;
RA Goytain A., Hines R.M., Quamme G.A.;
RT "Functional characterization of NIPA2, a selective Mg2+ transporter.";
RL Am. J. Physiol. 295:C944-C953(2008).
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Ba(2+), Mn(2+), Sr(2+) and Co(2+) but to a
CC much less extent than Mg(2+). May be a receptor for ligands (trioxilins
CC A3 and B3) from the hepoxilin pathway (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:18667602}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.36 mM for magnesium ions {ECO:0000269|PubMed:18667602};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Up-regulated by low magnesium ion levels.
CC {ECO:0000269|PubMed:18667602}.
CC -!- SIMILARITY: Belongs to the NIPA family. {ECO:0000305}.
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DR EMBL; AK035561; BAC29107.1; -; mRNA.
DR EMBL; AL663031; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL713958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466575; EDL33826.1; -; Genomic_DNA.
DR EMBL; BC139819; AAI39820.1; -; mRNA.
DR CCDS; CCDS24572.1; -.
DR RefSeq; NP_766112.1; NM_172524.3.
DR AlphaFoldDB; Q8BZF2; -.
DR STRING; 10090.ENSMUSP00000020679; -.
DR GlyGen; Q8BZF2; 4 sites.
DR PhosphoSitePlus; Q8BZF2; -.
DR PaxDb; Q8BZF2; -.
DR PRIDE; Q8BZF2; -.
DR ProteomicsDB; 252967; -.
DR Antibodypedia; 48488; 72 antibodies from 15 providers.
DR Ensembl; ENSMUST00000020679; ENSMUSP00000020679; ENSMUSG00000020411.
DR GeneID; 214112; -.
DR KEGG; mmu:214112; -.
DR UCSC; uc007ioa.1; mouse.
DR CTD; 348938; -.
DR MGI; MGI:2444671; Nipal4.
DR VEuPathDB; HostDB:ENSMUSG00000020411; -.
DR eggNOG; KOG2922; Eukaryota.
DR GeneTree; ENSGT00940000159087; -.
DR HOGENOM; CLU_012349_1_2_1; -.
DR InParanoid; Q8BZF2; -.
DR OMA; MGAGEVC; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; Q8BZF2; -.
DR TreeFam; TF313214; -.
DR Reactome; R-MMU-5223345; Miscellaneous transport and binding events.
DR BioGRID-ORCS; 214112; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q8BZF2; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8BZF2; protein.
DR Bgee; ENSMUSG00000020411; Expressed in urinary bladder urothelium and 123 other tissues.
DR Genevisible; Q8BZF2; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; IDA:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Magnesium; Membrane; Receptor;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..406
FT /note="Magnesium transporter NIPA4"
FT /id="PRO_0000284448"
FT TOPO_DOM 1..57
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..126
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..155
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..197
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..225
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..295
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 9
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 14
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 292
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 406 AA; 44059 MW; 39548AF196CF41DF CRC64;
MELRVANANG SCENGSIVSL YCSSQEVLCQ IVRGISPEEP YNATLITWQE RVRKKYGFYI
GVGLAFLSCF LIGTSVILKK KGLIRLVATG ATRAVNGGYG YLKDPMWWAG MATMSAGEVA
NFGAYAFAPA TVVTPLGALS VLISAIFSSY CLGESLNLLG KLGCVICMAG STVMVIHAPK
EEKVTTVAEM ASKMKDTGFI VFAVLLVVSC LILIFIVAPR YGQRNILIYI IICSVIGSFS
VTAVKGLGVT IRNFFQGLPV VRHPLPYILS LILGLSIIIQ VNFLNRALDI FNTSLVFPIY
YVFFTTVVVA SSIVLFKEWY TMSAVDIVGT LSGFVTIILG VFMLHAFKDL DINQISLPHT
HKNPTPAPAP EPTVIKLEDK NVLVDNIELA STPSPQQKPK VFMTDS
