NIPA8_ARATH
ID NIPA8_ARATH Reviewed; 441 AA.
AC Q8RWF4; Q9LSE5;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Probable magnesium transporter NIPA8;
GN OrderedLocusNames=At3g26670; ORFNames=MLJ15.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but
CC to a much less extent than Mg(2+) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Early endosome {ECO:0000250}. Note=Recruited to
CC the cell membrane in response to low extracellular magnesium.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NIPA (TC 2.A.7) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01731.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB026648; BAB01731.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77193.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77194.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77195.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64303.1; -; Genomic_DNA.
DR EMBL; AY093129; AAM13128.1; -; mRNA.
DR EMBL; BT006587; AAP31931.1; -; mRNA.
DR EMBL; AK226900; BAE98977.1; -; mRNA.
DR RefSeq; NP_001326342.1; NM_001338815.1.
DR RefSeq; NP_189301.1; NM_113578.4.
DR RefSeq; NP_850634.1; NM_180303.4.
DR RefSeq; NP_974367.1; NM_202638.2.
DR AlphaFoldDB; Q8RWF4; -.
DR STRING; 3702.AT3G26670.2; -.
DR PaxDb; Q8RWF4; -.
DR PRIDE; Q8RWF4; -.
DR ProteomicsDB; 251173; -.
DR EnsemblPlants; AT3G26670.1; AT3G26670.1; AT3G26670.
DR EnsemblPlants; AT3G26670.2; AT3G26670.2; AT3G26670.
DR EnsemblPlants; AT3G26670.3; AT3G26670.3; AT3G26670.
DR EnsemblPlants; AT3G26670.4; AT3G26670.4; AT3G26670.
DR GeneID; 822279; -.
DR Gramene; AT3G26670.1; AT3G26670.1; AT3G26670.
DR Gramene; AT3G26670.2; AT3G26670.2; AT3G26670.
DR Gramene; AT3G26670.3; AT3G26670.3; AT3G26670.
DR Gramene; AT3G26670.4; AT3G26670.4; AT3G26670.
DR KEGG; ath:AT3G26670; -.
DR Araport; AT3G26670; -.
DR TAIR; locus:2090822; AT3G26670.
DR eggNOG; KOG2922; Eukaryota.
DR HOGENOM; CLU_054115_0_0_1; -.
DR InParanoid; Q8RWF4; -.
DR OMA; FQEYQVL; -.
DR OrthoDB; 754939at2759; -.
DR PhylomeDB; Q8RWF4; -.
DR PRO; PR:Q8RWF4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RWF4; baseline and differential.
DR Genevisible; Q8RWF4; AT.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
DR Pfam; PF05653; Mg_trans_NIPA; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..441
FT /note="Probable magnesium transporter NIPA8"
FT /id="PRO_0000430296"
FT TOPO_DOM 1..4
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..25
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..79
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..105
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..144
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..184
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..222
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..255
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..288
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 313..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 441 AA; 48409 MW; 43B04EEB89C9024F CRC64;
MGEWVIGAFI NIFGSVAINF GTNLLKLGHN ERERLALQDG GGKMPLKPII HNQTWRVGIL
VFLLGNCLNF ISFGYAAQSL LAALGSIQFV SNIAFAYVVL NKMVTVKVLV ATAFIVLGNV
FLVAFGNHQS PVFTPEQLAE KYSNVTFLVY CGILILIVAV HHFLYRKGEV LISTPGQEIS
SYWKMLLPFS YAVVSGAIGS CSVLFAKSLS NLLRLAMSSS YQLHSWFTYS MLLLFLSTAG
FWMTRLNEGL SLYDAILIVP MFQIAWTFFS ICTGCIYFQE FQVFDALRTT MFILGMMCVF
IGISLLAPDD TRGNETKDNS SSLDSIVSSS VPTEEDRLIP QSSEDGHSKD TRVVVQGMYM
KAADLIAKTK TACLAALGFG EDSINASAIL VMPMVSSKIT GFRGNGLERA KILSMRGSGW
SKLAMEEEGT RMLEKTISSK A
