NIPA9_ARATH
ID NIPA9_ARATH Reviewed; 344 AA.
AC Q8RWH8; Q9LYH7;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Probable magnesium transporter NIPA9;
GN OrderedLocusNames=At5g11960; ORFNames=F14F18_130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Acts as a Mg(2+) transporter. Can also transport other
CC divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but
CC to a much less extent than Mg(2+) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Early endosome {ECO:0000250}. Note=Recruited to
CC the cell membrane in response to low extracellular magnesium.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NIPA (TC 2.A.7) family. {ECO:0000305}.
CC -!- CAUTION: Lacks one of the 9 transmembrane regions, which are conserved
CC features of the family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87669.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL163812; CAB87669.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91745.1; -; Genomic_DNA.
DR EMBL; AY093077; AAM13076.1; -; mRNA.
DR EMBL; AY128765; AAM91165.1; -; mRNA.
DR PIR; T48555; T48555.
DR RefSeq; NP_196757.2; NM_121234.3.
DR AlphaFoldDB; Q8RWH8; -.
DR iPTMnet; Q8RWH8; -.
DR PaxDb; Q8RWH8; -.
DR PRIDE; Q8RWH8; -.
DR ProteomicsDB; 251059; -.
DR EnsemblPlants; AT5G11960.1; AT5G11960.1; AT5G11960.
DR GeneID; 831069; -.
DR Gramene; AT5G11960.1; AT5G11960.1; AT5G11960.
DR KEGG; ath:AT5G11960; -.
DR Araport; AT5G11960; -.
DR TAIR; locus:2143044; AT5G11960.
DR eggNOG; ENOG502QSWV; Eukaryota.
DR HOGENOM; CLU_048190_0_0_1; -.
DR InParanoid; Q8RWH8; -.
DR OMA; TGALAWE; -.
DR OrthoDB; 1566086at2759; -.
DR PhylomeDB; Q8RWH8; -.
DR PRO; PR:Q8RWH8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8RWH8; baseline and differential.
DR Genevisible; Q8RWH8; AT.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015693; P:magnesium ion transport; ISS:UniProtKB.
DR InterPro; IPR008521; Mg_trans_NIPA.
DR PANTHER; PTHR12570; PTHR12570; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Ion transport; Magnesium; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..344
FT /note="Probable magnesium transporter NIPA9"
FT /id="PRO_0000430297"
FT TOPO_DOM 1..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..125
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..191
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..265
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..344
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 344 AA; 37388 MW; 0D941E9A8FC177EA CRC64;
MWESICLTLA ATAGNNIGKV LQKKGTIILP PLSLKLKVLR AYAENKPWAL GFLMDIVGAL
LMLRALSLAP VSVVQPVSGC GLAILSVFSH FYLKEVMNVF DWIGITVAGI GTIGVGAGGE
EQEASLISVF QLLWLALVVA ILFVLLNAWL HIFKRQRREQ ELGEYEVVEE IIYGLESGIL
FGMASVVSKM GFVFVEQGFS TMFIPMCISI SICCSGTGFF YQTRGLKHGR AIVVSTCAAV
ASIVTGVVAG MFALGEKLPT SPSGRLLLLL GWLLIMLGVV LLVTSSRLIR HLPRSFRRSR
QTSLERGFNI RRTTSHTPKD TNPSAVIQAA TLHHLLSSPS KDKD
