ID   NIRA_EMENI              Reviewed;         892 AA.
AC   P28348; C8VQM8; Q5BH82;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Nitrogen assimilation transcription factor nirA;
GN   Name=nirA; ORFNames=AN0098;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1922075; DOI=10.1128/mcb.11.11.5746-5755.1991;
RA   Burger G., Strauss J., Scazzocchio C., Lang B.F.;
RT   "nirA, the pathway-specific regulatory gene of nitrate assimilation in
RT   Aspergillus nidulans, encodes a putative GAL4-type zinc finger protein and
RT   contains four introns in highly conserved regions.";
RL   Mol. Cell. Biol. 11:5746-5755(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
CC   -!- FUNCTION: Pathway-specific regulatory gene of nitrate assimilation; it
CC       activates the transcription of the genes for nitrate and nitrite
CC       reductases (niaD and niiA).
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAA65276.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M68900; AAA33317.1; -; Genomic_DNA.
DR   EMBL; AACD01000004; EAA65276.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BN001308; CBF90203.1; -; Genomic_DNA.
DR   PIR; A41697; A41697.
DR   RefSeq; XP_657702.1; XM_652610.1.
DR   AlphaFoldDB; P28348; -.
DR   STRING; 162425.CADANIAP00002651; -.
DR   MetOSite; P28348; -.
DR   PRIDE; P28348; -.
DR   EnsemblFungi; CBF90203; CBF90203; ANIA_00098.
DR   EnsemblFungi; EAA65276; EAA65276; AN0098.2.
DR   GeneID; 2875872; -.
DR   KEGG; ani:AN0098.2; -.
DR   VEuPathDB; FungiDB:AN0098; -.
DR   eggNOG; ENOG502QW0K; Eukaryota.
DR   HOGENOM; CLU_007003_6_0_1; -.
DR   InParanoid; P28348; -.
DR   OMA; IAHTACT; -.
DR   OrthoDB; 917119at2759; -.
DR   Proteomes; UP000000560; Chromosome VIII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IDA:AspGD.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:CACAO.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006338; P:chromatin remodeling; IMP:AspGD.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0006808; P:regulation of nitrogen utilization; IMP:AspGD.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   4: Predicted;
KW   Activator; DNA-binding; Metal-binding; Nitrate assimilation; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..892
FT                   /note="Nitrogen assimilation transcription factor nirA"
FT                   /id="PRO_0000114959"
FT   DNA_BIND        42..70
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          646..714
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          731..761
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          842..892
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        12..32
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        649..714
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        742..761
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        852..867
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        869..892
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   892 AA;  99449 MW;  6EFD105203A53F59 CRC64;
     MGEKLDPELS SDGPHTKSSS KGQGTSTDNA PASKRRCVST ACIACRRRKS KCDGNLPSCA
     ACSSVYHTTC VYDPNSDHRR KGVYKKDTDT LRTKNSTLLT LIQALLNYEE EDAFDLVRQI
     RSCDNLEDVA QSLVNQEKKS SGWLSNAVIH EENDIAQTDQ FESELAGKMS NLVLDGSRKF
     IGGTSNLIFL PPGSELNEFK PGLATNGDLE GSVTRWTTVT DDQQLISHLL TMYFSWHYPF
     FTTLSKELFY RDYSRGVPSQ YCSSLLVNTM LALGCHFSSW PGAREDPDNS ATAGDHFFKE
     AKRLILDNDE LVNSKLCTVQ ALALMSVREA GCGREGKGWV YSGMSFRMAF DLGLNLESSS
     LRDLSEEEID ARRITFWGCF LFDKCWSNYL GRQPQFTTAN TSVSAVDILP NEESTLWSPY
     SDMGPSREYA QPSRTRAVAD QISQLCKISG DLVVFFYDLA PKEKPSSKQL ELKKLSEIHT
     RLEAWKKGLP KELEPREGQL PQALLMHMFY QLLLIHLYRP FLKYTKSTSP LPQHVSPRKL
     CTQAAAAISK LLRLYKRTYG FKQICNIAVY IAHTALTIHL LNLPEKNAQR DVIHGLRHLE
     EMGESWLCAR RTLRILDISA SKWQVQLPRE AVIVFEQTHA RWGSWGPWDQ AASPSTTSDS
     PPSVSSQSVV ATTDLSQPVS QSAGNQPANP SMGTSPNLTQ PVASQYSSTP SGPVSVSAMR
     AVQRSFSAQL AHNEARQPEP TYLRPVSTSY GPVPSTQSAQ EQWYSPTEAQ FRAFTAAHSM
     PTTSAQSPLT TFDTPENLVE ESQDWWSRDV NALQLGAEDW TQNWNNGLPT TSADWRYVDN
     VPNIPSTSAP DADYKPPQPP PNMARPNQYP TDPVANVNSN QTNMIFPGSF QR