NIRC_SALTY
ID NIRC_SALTY Reviewed; 269 AA.
AC P25926;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Nitrite transporter NirC;
GN Name=nirC; OrderedLocusNames=STM3476;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1987123; DOI=10.1128/jb.173.1.325-333.1991;
RA Wu J.Y., Siegel L.M., Kredich N.M.;
RT "High-level expression of Escherichia coli NADPH-sulfite reductase:
RT requirement for a cloned cysG plasmid to overcome limiting siroheme
RT cofactor.";
RL J. Bacteriol. 173:325-333(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Catalyzes nitrite uptake and nitrite export across the
CC cytoplasmic membrane. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the FNT transporter (TC 2.A.44) family.
CC {ECO:0000305}.
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DR EMBL; M64606; AAA27040.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22338.1; -; Genomic_DNA.
DR PIR; A39200; A39200.
DR RefSeq; NP_462379.1; NC_003197.2.
DR RefSeq; WP_000493569.1; NC_003197.2.
DR AlphaFoldDB; P25926; -.
DR SMR; P25926; -.
DR DIP; DIP-60084N; -.
DR STRING; 99287.STM3476; -.
DR PaxDb; P25926; -.
DR EnsemblBacteria; AAL22338; AAL22338; STM3476.
DR GeneID; 1254999; -.
DR KEGG; stm:STM3476; -.
DR PATRIC; fig|99287.12.peg.3674; -.
DR HOGENOM; CLU_036896_2_1_6; -.
DR OMA; SIRPLVM; -.
DR PhylomeDB; P25926; -.
DR BioCyc; SENT99287:STM3476-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015499; F:formate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015724; P:formate transport; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000292; For/NO2_transpt.
DR InterPro; IPR024002; For/NO2_transpt_CS.
DR PANTHER; PTHR30520; PTHR30520; 1.
DR Pfam; PF01226; Form_Nir_trans; 1.
DR TIGRFAMs; TIGR00790; fnt; 1.
DR PROSITE; PS01005; FORMATE_NITRITE_TP_1; 1.
DR PROSITE; PS01006; FORMATE_NITRITE_TP_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Nitrate assimilation;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..269
FT /note="Nitrite transporter NirC"
FT /id="PRO_0000094725"
FT TOPO_DOM 1..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..58
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..107
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..153
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..230
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..269
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 5
FT /note="T -> S (in Ref. 1; AAA27040)"
FT /evidence="ECO:0000305"
FT CONFLICT 12..19
FT /note="NAARIARL -> KLRASAPV (in Ref. 1; AAA27040)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 269 AA; 28636 MW; E6208777CCC950F0 CRC64;
MFTDTINKCA ANAARIARLS ANNPLGFWVS SAMAGAYVGL GIILIFTLGN LLDPSVRPLV
MGATFGIALT LVIIAGSELF TGHTMFLTLG VKAGTISHGQ MWAILPQTWL GNLVGSVFVA
LLYSWGGGSL LPVDTSIVHS VALAKTTAPA TVLFFKGALC NWLVCLAIWM AIRTEGTAKF
LAIWWCLLAF IASGYEHSVA NMTLFALSWF GHHSDAYTLA GIGHNLLWVT LGNTLSGVVF
MGLGYWYATP KSERPAPAKI NQPEAAANN
