NIRF_PSEAE
ID NIRF_PSEAE Reviewed; 392 AA.
AC Q51480;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Protein NirF;
GN Name=nirF; OrderedLocusNames=PA0516;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8566817; DOI=10.1016/0378-1119(95)00641-9;
RA Kawasaki S., Arai H., Igarashi Y., Kodama T.;
RT "Sequencing and characterization of the downstream region of the genes
RT encoding nitrite reductase and cytochrome c-551 (nirSM) from Pseudomonas
RT aeruginosa: identification of the gene necessary for biosynthesis of heme
RT d1.";
RL Gene 167:87-91(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Required for the biosynthesis of heme d1 of nitrite
CC reductase. Could have a dehydrogenase activity yielding
CC sirohydrochlorin from precorrin-2 or dehydrogenation of propionate side
CC chain C17.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR EMBL; D50473; BAA09066.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG03905.1; -; Genomic_DNA.
DR PIR; JC4553; JC4553.
DR RefSeq; NP_249207.1; NC_002516.2.
DR RefSeq; WP_003113241.1; NZ_QZGE01000010.1.
DR PDB; 6TV2; X-ray; 1.56 A; A/B/C/D/E/F/G/H=18-392.
DR PDB; 6TV9; X-ray; 1.89 A; A/B/C/D/E/F/G/H=18-392.
DR PDBsum; 6TV2; -.
DR PDBsum; 6TV9; -.
DR AlphaFoldDB; Q51480; -.
DR SMR; Q51480; -.
DR STRING; 287.DR97_3484; -.
DR PaxDb; Q51480; -.
DR PRIDE; Q51480; -.
DR EnsemblBacteria; AAG03905; AAG03905; PA0516.
DR GeneID; 882198; -.
DR KEGG; pae:PA0516; -.
DR PATRIC; fig|208964.12.peg.546; -.
DR PseudoCAP; PA0516; -.
DR HOGENOM; CLU_040920_0_0_6; -.
DR InParanoid; Q51480; -.
DR OMA; KMPHLRG; -.
DR PhylomeDB; Q51480; -.
DR BioCyc; PAER208964:G1FZ6-521-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR Gene3D; 2.140.10.20; -; 1.
DR InterPro; IPR003143; Cyt_cd1_C_sf.
DR InterPro; IPR011048; Haem_d1_sf.
DR SUPFAM; SSF51004; SSF51004; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome.
FT CHAIN 1..392
FT /note="Protein NirF"
FT /id="PRO_0000096858"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 35..38
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 39..44
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 45..48
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 49..54
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 61..67
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 71..78
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 81..87
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 88..91
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 92..98
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 102..108
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 114..119
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 120..123
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 124..129
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 135..140
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 153..159
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 160..162
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 163..168
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 169..172
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 173..178
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 182..184
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 186..190
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 197..202
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 208..223
FT /evidence="ECO:0007829|PDB:6TV2"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 231..235
FT /evidence="ECO:0007829|PDB:6TV2"
FT HELIX 247..249
FT /evidence="ECO:0007829|PDB:6TV2"
FT HELIX 253..255
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 260..266
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 268..277
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 278..281
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 282..288
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 293..298
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 302..309
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 317..321
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 322..324
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 327..331
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 337..342
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 344..347
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 349..353
FT /evidence="ECO:0007829|PDB:6TV2"
FT HELIX 354..356
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 358..363
FT /evidence="ECO:0007829|PDB:6TV2"
FT TURN 364..367
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 368..374
FT /evidence="ECO:0007829|PDB:6TV2"
FT STRAND 376..383
FT /evidence="ECO:0007829|PDB:6TV2"
FT HELIX 384..388
FT /evidence="ECO:0007829|PDB:6TV2"
SQ SEQUENCE 392 AA; 43338 MW; E515019C9E15011D CRC64;
MNLRPLAPLL LTLLAGCSQQ PPLRGSGDLG VLIERADGSV QILDGTAKTS LARVEGLGDL
SHASLVFSRD QRYAYVFGRD GGLTKLDLLA QRIDKRLIQG GNSIGGAISQ DGRLVAVSNY
EPGGVKVFDS RTLELVAEIP ATRLPGQDRN SRVVGLVDAP GQRFVFSLFD SGEIWIADFS
QGDTPHLTRF RDIGKQPYDA LISPDGRYYM AGLFGEDGMA QLDLWHPERG VRRVLGDYGR
GQRKLPVYKM PHLEGWTIAS DQAFVPAVGH HQVLVLDARD WKQTDAIDVA GQPVFVMTRP
DDRQIWVNFA YPDNDKVQVI DSETHEVIET LRPGPGVLHM EFSGRGDQVW ISVRDADQLQ
VWDPYRLKRI GSLPARSPSG IFFSHRAQHI GL
