ID   NIRG_PARPN              Reviewed;         157 AA.
AC   I6TCK3;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=Siroheme decarboxylase NirG subunit {ECO:0000305};
DE            EC=4.1.1.111 {ECO:0000269|PubMed:21969545};
GN   Name=nirG {ECO:0000303|PubMed:21969545};
OS   Paracoccus pantotrophus (Thiosphaera pantotropha).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus.
OX   NCBI_TaxID=82367;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY,
RP   AND SUBUNIT.
RC   STRAIN=ATCC 35512 / DSM 2944 / CIP 106514 / LMD 82.5 / NBRC 102493 / NCCB
RC   82005 / GB17;
RX   PubMed=21969545; DOI=10.1073/pnas.1108228108;
RA   Bali S., Lawrence A.D., Lobo S.A., Saraiva L.M., Golding B.T., Palmer D.J.,
RA   Howard M.J., Ferguson S.J., Warren M.J.;
RT   "Molecular hijacking of siroheme for the synthesis of heme and d1 heme.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:18260-18265(2011).
CC   -!- FUNCTION: Involved in heme d1 biosynthesis. Catalyzes the
CC       decarboxylation of siroheme into didecarboxysiroheme (PubMed:21969545).
CC       Siroheme is probably decarboxylated to monodecarboxysiroheme, which is
CC       in turn decarboxylated to didecarboxysiroheme (PubMed:21969545).
CC       {ECO:0000269|PubMed:21969545}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + siroheme = 12,18-didecarboxysiroheme + 2 CO2;
CC         Xref=Rhea:RHEA:19093, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:60052, ChEBI:CHEBI:140497; EC=4.1.1.111;
CC         Evidence={ECO:0000269|PubMed:21969545};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism.
CC       {ECO:0000269|PubMed:21969545}.
CC   -!- SUBUNIT: Forms a complex composed of NirDL, NirG and NirH. All proteins
CC       are required for the total conversion of siroheme to
CC       didecarboxysiroheme. {ECO:0000269|PubMed:21969545}.
CC   -!- SIMILARITY: Belongs to the Ahb/Nir family. {ECO:0000305}.
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DR   EMBL; JQ922109; AFM94358.1; -; Genomic_DNA.
DR   AlphaFoldDB; I6TCK3; -.
DR   SMR; I6TCK3; -.
DR   BioCyc; MetaCyc:MON-18862; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000485; AsnC-type_HTH_dom.
DR   InterPro; IPR040523; AsnC_trans_reg2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF17805; AsnC_trans_reg2; 1.
DR   Pfam; PF13404; HTH_AsnC-type; 1.
PE   1: Evidence at protein level;
KW   Lyase.
FT   CHAIN           1..157
FT                   /note="Siroheme decarboxylase NirG subunit"
FT                   /id="PRO_0000450516"
SQ   SEQUENCE   157 AA;  17183 MW;  74E5E9FC1A44B804 CRC64;
     MRRQTLPPEA LDDTDRAILN RLQEGFPLTP RPFDDAGAAL GLTGAQLIER LERLRAIGAI
     TRFGPFYDAA AMGGAFCLCA LSAPQADFDR IAALVNAHPE VAHNYARDHA LNMWFVLATA
     TPEGIAETAG RIEAETGLTV WRFPKLREFF IGFRVAA