NIRH_PARPN
ID NIRH_PARPN Reviewed; 160 AA.
AC I6UF18;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 03-AUG-2022, entry version 26.
DE RecName: Full=Siroheme decarboxylase NirH subunit {ECO:0000305};
DE EC=4.1.1.111 {ECO:0000269|PubMed:21969545};
GN Name=nirH {ECO:0000303|PubMed:21969545};
OS Paracoccus pantotrophus (Thiosphaera pantotropha).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Paracoccus.
OX NCBI_TaxID=82367;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY,
RP AND SUBUNIT.
RC STRAIN=ATCC 35512 / DSM 2944 / CIP 106514 / LMD 82.5 / NBRC 102493 / NCCB
RC 82005 / GB17;
RX PubMed=21969545; DOI=10.1073/pnas.1108228108;
RA Bali S., Lawrence A.D., Lobo S.A., Saraiva L.M., Golding B.T., Palmer D.J.,
RA Howard M.J., Ferguson S.J., Warren M.J.;
RT "Molecular hijacking of siroheme for the synthesis of heme and d1 heme.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:18260-18265(2011).
CC -!- FUNCTION: Involved in heme d1 biosynthesis. Catalyzes the
CC decarboxylation of siroheme into didecarboxysiroheme (PubMed:21969545).
CC Siroheme is probably decarboxylated to monodecarboxysiroheme, which is
CC in turn decarboxylated to didecarboxysiroheme (PubMed:21969545).
CC {ECO:0000269|PubMed:21969545}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + siroheme = 12,18-didecarboxysiroheme + 2 CO2;
CC Xref=Rhea:RHEA:19093, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:60052, ChEBI:CHEBI:140497; EC=4.1.1.111;
CC Evidence={ECO:0000269|PubMed:21969545};
CC -!- PATHWAY: Porphyrin-containing compound metabolism.
CC {ECO:0000269|PubMed:21969545}.
CC -!- SUBUNIT: Forms a complex composed of NirDL, NirG and NirH. All proteins
CC are required for the total conversion of siroheme to
CC didecarboxysiroheme. {ECO:0000269|PubMed:21969545}.
CC -!- SIMILARITY: Belongs to the Ahb/Nir family. {ECO:0000305}.
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DR EMBL; JQ922110; AFM94359.1; -; Genomic_DNA.
DR AlphaFoldDB; I6UF18; -.
DR SMR; I6UF18; -.
DR BioCyc; MetaCyc:MON-18863; -.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR InterPro; IPR000485; AsnC-type_HTH_dom.
DR InterPro; IPR040523; AsnC_trans_reg2.
DR Pfam; PF17805; AsnC_trans_reg2; 1.
DR Pfam; PF13404; HTH_AsnC-type; 1.
PE 1: Evidence at protein level;
KW Lyase.
FT CHAIN 1..160
FT /note="Siroheme decarboxylase NirH subunit"
FT /id="PRO_0000450517"
SQ SEQUENCE 160 AA; 17634 MW; C2F9E8A7B8CEA1BB CRC64;
MTAPDDTDRR LIAATQAGLP LDEAPYARIA AELGLTETQV ITRLSILHAQ GVIRRIAIAP
NHYALGMIAN GMSVWDVDDA QAEALGERIG ALDFVTHCYL RPRAPVWRYN LFAMLHGQSR
AEVEQKRAQV RALLGAACRA DDILYSTRIL KKTGLRLKDG