ID   NIRI_PARDP              Reviewed;         681 AA.
AC   Q51699; A1B4X9;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 2.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Protein NirI;
DE   Flags: Precursor;
GN   Name=nirI; OrderedLocusNames=Pden_2486;
OS   Paracoccus denitrificans (strain Pd 1222).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus.
OX   NCBI_TaxID=318586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pd 1222;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S.,
RA   Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M.,
RA   Richardson P.;
RT   "Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-143.
RX   PubMed=7747927; DOI=10.1007/bf00871635;
RA   de Boer A.P.N., Reijnders W.N.M., Kuenen J.G., Stouthamer A.H.,
RA   van Spanning R.J.M.;
RT   "Isolation, sequencing and mutational analysis of a gene cluster involved
RT   in nitrite reduction in Paracoccus denitrificans.";
RL   Antonie Van Leeuwenhoek 66:111-127(1994).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: To P.stutzeri NosR. {ECO:0000305}.
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DR   EMBL; CP000489; ABL70573.1; -; Genomic_DNA.
DR   EMBL; U05002; AAA93117.1; -; Genomic_DNA.
DR   RefSeq; WP_011748766.1; NC_008686.1.
DR   AlphaFoldDB; Q51699; -.
DR   STRING; 318586.Pden_2486; -.
DR   PRIDE; Q51699; -.
DR   EnsemblBacteria; ABL70573; ABL70573; Pden_2486.
DR   KEGG; pde:Pden_2486; -.
DR   eggNOG; COG0348; Bacteria.
DR   eggNOG; COG3901; Bacteria.
DR   HOGENOM; CLU_013077_0_0_5; -.
DR   OMA; PQCGREC; -.
DR   Proteomes; UP000000361; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR007329; FMN-bd.
DR   InterPro; IPR011399; NosR.
DR   Pfam; PF12801; Fer4_5; 2.
DR   PIRSF; PIRSF036354; NosR; 1.
DR   SMART; SM00900; FMN_bind; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..681
FT                   /note="Protein NirI"
FT                   /id="PRO_0000096861"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        436..456
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        468..488
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        535..555
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        568..588
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        100..102
FT                   /note="PQG -> RRA (in Ref. 2; AAA93117)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   681 AA;  73240 MW;  408A821ECD9DA39D CRC64;
     MAMPGKSSHA PSRLLLALLT LILLALPARP EPLAAPDAAL AQALFGADGP VVLDRRETPV
     PGWGVSRDGR MLGVIGSTWE IAATTGYSGK PLDVLVAVTP QGVIAGARLV RQTEPVLSLG
     ISEAHIAAYV DGFRGVDLSQ GEGQGRALPD VISRATVSTG VIRDGILRSA RVLAQAQGLG
     GGGIDRVGYR PATWADLLAE GAFGHALVSM ADAARAFAGA KVPVEPSDAP FLDLYAGLID
     PPTVGRNLLG AQDFTAAAGA LQPGQALVAV LSRGLYSPRG TEWRRSGRFE RIAFEQGALR
     IEPDDGDFVM VEKLALPDAP AFKEISLFRL NIDPLAGGID PRRPFDLVVT ATRPLAAGDE
     AALPIRATIR LPEAYAVAEA PAVPLWQEFW LKKIPGIAVV AAMLFVLALI LFGQEALVRR
     PMLWRRVRLG FLATTLVVLG WGLNGQLSVV QVVAFLNALL SGFRWETFLI EPIIFLIWSA
     VALGLLFWGR GVFCGWLCPF GALQELANAA AQRLGVRQIA VPQALHERLW VIKYTLFVAI
     VALSFYSMEQ ALILAEVEPF KTVISMRFLR AWPFVLFALA VLAGGLFIER FYCRYLCPLG
     AGLAIPAKLK IFDWLRRRPQ CGRECRLCET KCTVGAIDPL GRINPNECVL CLRCQVVMND
     PGTCPVLKRR ARSAAPTGDA P