ID   NIRQ_PSEST              Reviewed;         275 AA.
AC   Q02441;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Denitrification regulatory protein NirQ;
GN   Name=nirQ;
OS   Pseudomonas stutzeri (Pseudomonas perfectomarina).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14405 / JCM 20778 / CIP 107696 / IAM 12931 / LMG 2243 / NCIMB
RC   568 / 218 / ZoBell 632;
RX   PubMed=1468562; DOI=10.1016/0014-5793(92)81495-8;
RA   Juengst A., Zumft W.G.;
RT   "Interdependence of respiratory NO reduction and nitrite reduction revealed
RT   by mutagenesis of nirQ, a novel gene in the denitrification gene cluster of
RT   Pseudomonas stutzeri.";
RL   FEBS Lett. 314:308-314(1992).
CC   -!- FUNCTION: Activator of nitrite and nitric oxide reductases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Under denitrifying conditions.
CC   -!- SIMILARITY: Belongs to the CbbQ/NirQ/NorQ/GpvN family. {ECO:0000305}.
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DR   EMBL; X53676; CAA78998.1; -; Genomic_DNA.
DR   PIR; S27012; S27012.
DR   AlphaFoldDB; Q02441; -.
DR   SMR; Q02441; -.
DR   STRING; 32042.PstZobell_00972; -.
DR   eggNOG; COG0714; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR   InterPro; IPR013615; CbbQ_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF07728; AAA_5; 1.
DR   Pfam; PF08406; CbbQ_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   Activator; ATP-binding; Cytoplasm; DNA-binding; Nucleotide-binding;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..275
FT                   /note="Denitrification regulatory protein NirQ"
FT                   /id="PRO_0000219570"
FT   DNA_BIND        249..268
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   BINDING         47..54
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         107..114
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   275 AA;  30544 MW;  EC5FA0E9A1CB03D9 CRC64;
     MRYLPVNAIE IPTTAGTPDA PFYQPLGNEE QLFQQAWQHG MPVLIKGPTG CGKTRFVQHM
     AHRLNLPLYT VACHDDLSAA DLVGRHLIGA QGTWWQDGPL TRAVREGGIC YLDEVVEARQ
     DTAVVLHPLA DDRRELFIER TGEALKAPPG FMLVVSYNPG YQNLLKGMKP STRQRFVAMR
     FDYPPTAEEE RIVANEAQVD AALAAQVVKL GQALRRLEQH DLEEVASTRL LIFTARMIRS
     GMTPRQACLA CLAEPLSDDP QTVAALMDVV YVHFG