ID   NIR_LEPMC               Reviewed;         185 AA.
AC   P43504;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Nitrite reductase [NAD(P)H];
DE            EC=1.7.1.4;
DE   Flags: Fragment;
GN   Name=NIIA;
OS   Leptosphaeria maculans (Blackleg fungus) (Phoma lingam).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Leptosphaeriaceae;
OC   Leptosphaeria; Leptosphaeria maculans species complex.
OX   NCBI_TaxID=5022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Isolate 19;
RX   PubMed=7789806; DOI=10.1016/0378-1119(95)00169-7;
RA   Williams R.S.B., Davis M.A., Howlett B.J.;
RT   "The nitrate and nitrite reductase-encoding genes of Leptosphaeria maculans
RT   are closely linked and transcribed in the same direction.";
RL   Gene 158:153-154(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H2O + 3 NADP(+) + NH4(+) = 5 H(+) + 3 NADPH + nitrite;
CC         Xref=Rhea:RHEA:24632, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16301, ChEBI:CHEBI:28938, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.7.1.4;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H2O + 3 NAD(+) + NH4(+) = 5 H(+) + 3 NADH + nitrite;
CC         Xref=Rhea:RHEA:24628, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16301, ChEBI:CHEBI:28938, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.7.1.4;
CC   -!- COFACTOR:
CC       Name=siroheme; Xref=ChEBI:CHEBI:60052; Evidence={ECO:0000250};
CC       Note=Binds 1 siroheme per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000250};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
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DR   EMBL; U18793; AAA82739.1; -; Genomic_DNA.
DR   PIR; PC4032; PC4032.
DR   AlphaFoldDB; P43504; -.
DR   SMR; P43504; -.
DR   UniPathway; UPA00653; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0106316; F:nitrite reductase NADH activity; IEA:RHEA.
DR   GO; GO:0106314; F:nitrite reductase NADPH activity; IEA:RHEA.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; FAD; Flavoprotein; Heme; Iron; Iron-sulfur; Metal-binding; NADP;
KW   Nitrate assimilation; Oxidoreductase.
FT   CHAIN           1..>185
FT                   /note="Nitrite reductase [NAD(P)H]"
FT                   /id="PRO_0000199959"
FT   REGION          14..47
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        33..47
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         72..107
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   NON_TER         185
SQ   SEQUENCE   185 AA;  20756 MW;  19D67F97D386E7F9 CRC64;
     MAATLPLLTQ GIEPVSGESY SPPGERHVQA TWGSKDGNIS NTEWNDPEAN RLPEKVAELE
     KKGELNNSHP RRRVVVVGLG MVGVAFIEKL MKYDIKRREY DIIVIGEEPH LAYNRVGLTS
     FFQHRQVENL YLNPQEWYSS MPEDSLHYHL NTLVTEIDSE NKTVKTSSGQ AVSYDILVLA
     TGSSS