NIR_SYNE7
ID NIR_SYNE7 Reviewed; 512 AA.
AC P39661; Q31NU9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ferredoxin--nitrite reductase;
DE EC=1.7.7.1;
GN Name=nirA; OrderedLocusNames=Synpcc7942_1240;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Omata T.;
RL Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8490140; DOI=10.1007/bf00023618;
RA Luque I., Flores E., Herrero A.;
RT "Nitrite reductase gene from Synechococcus sp. PCC 7942: homology between
RT cyanobacterial and higher-plant nitrite reductases.";
RL Plant Mol. Biol. 21:1201-1205(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 468-512.
RA Omata T.;
RT "Cloning and characterization of the nrtA gene that encodes a 45-kDa
RT protein involved in nitrate transport in the cyanobacterium Synechococcus
RT PCC 7942.";
RL Plant Cell Physiol. 32:151-157(1991).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H2O + NH4(+) + 6 oxidized [2Fe-2S]-[ferredoxin] = 8 H(+) +
CC nitrite + 6 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:18041,
CC Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16301, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.7.7.1;
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000305}.
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DR EMBL; D12723; BAA02217.1; -; Genomic_DNA.
DR EMBL; X67680; CAA47912.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB57270.1; -; Genomic_DNA.
DR PIR; S33530; PQ0646.
DR RefSeq; WP_011242624.1; NC_007604.1.
DR AlphaFoldDB; P39661; -.
DR SMR; P39661; -.
DR STRING; 1140.Synpcc7942_1240; -.
DR PRIDE; P39661; -.
DR EnsemblBacteria; ABB57270; ABB57270; Synpcc7942_1240.
DR KEGG; syf:Synpcc7942_1240; -.
DR eggNOG; COG0155; Bacteria.
DR HOGENOM; CLU_015667_2_2_3; -.
DR OMA; IKISGCM; -.
DR OrthoDB; 1588103at2; -.
DR BioCyc; MetaCyc:SYNPCC7942_1240-MON; -.
DR BioCyc; SYNEL:SYNPCC7942_1240-MON; -.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0048307; F:ferredoxin-nitrite reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.413.10; -; 2.
DR InterPro; IPR012798; Cbl_synth_CobG-like.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR Pfam; PF01077; NIR_SIR; 2.
DR Pfam; PF03460; NIR_SIR_ferr; 2.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF55124; SSF55124; 2.
DR SUPFAM; SSF56014; SSF56014; 2.
DR TIGRFAMs; TIGR02435; CobG; 1.
DR PROSITE; PS00365; NIR_SIR; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Electron transport; Heme; Iron; Iron-sulfur; Metal-binding;
KW Nitrate assimilation; Oxidoreductase; Transport.
FT CHAIN 1..512
FT /note="Ferredoxin--nitrite reductase"
FT /id="PRO_0000199957"
FT BINDING 396
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 402
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 437
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 441
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 441
FT /ligand="siroheme"
FT /ligand_id="ChEBI:CHEBI:60052"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 512 AA; 56541 MW; 9F469CAF60E816F0 CRC64;
MAQATATTEK LNKFEKLKLE KDGLAVRDQI QHFASIGWEA MDPGDREHRL KWLGIFWRPV
TPGRFMARLR IPSGILQSQQ LNALANFLQR YGDQASIDIT TRQNLQLRGL LLEDTPEFLE
RLHAVGLTSV QSGMDNVRNI TGSPVAGLDA AELFDTRSLI QALQDDLTAA GQGNSEFTNL
PRKFNIAIEG GRDNSIHAEI NDLAFTPAYQ DGTLGFNVWV GGFFSSTRVA PAIPLNAWVP
ADHSVIRLSR AILEVFRDNG SRGNRQKTRL MWLIDEWGIE RFRQVVSEAY GAPLAAAAPE
LMDWEKRDFL GVHPQKQAGL NFVGLHVPVG RLTTEDLYEL ARLADTYGQG EVRLTVEQNV
ILTHIPDAQL PTLLAEPLLT RFSPQPAPLS RGTVSCTGSQ YCNFALIETK QRAIAIAQSL
EAELDLPRPV RIHWTGCPNS CGQPQVADIG LMGAKVRKDG QMVEGVDIFL GGKVGYDAHL
GEKAMTGVAC EDLPDVLRQL LIERFGAQAR SH
