NIS1_COLHI
ID NIS1_COLHI Reviewed; 144 AA.
AC H1VZX9;
DT 31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=Necrosis-inducing secreted protein 1 {ECO:0000303|PubMed:22352720};
DE AltName: Full=Secreted effector NIS1 {ECO:0000303|PubMed:22352720};
DE Flags: Precursor;
GN Name=NIS1 {ECO:0000303|PubMed:22352720}; ORFNames=CH063_03697, CH63R_07994;
OS Colletotrichum higginsianum (strain IMI 349063) (Crucifer anthracnose
OS fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC Colletotrichum destructivum species complex.
OX NCBI_TaxID=759273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IMI 349063;
RX PubMed=22885923; DOI=10.1038/ng.2372;
RA O'Connell R.J., Thon M.R., Hacquard S., Amyotte S.G., Kleemann J.,
RA Torres M.F., Damm U., Buiate E.A., Epstein L., Alkan N., Altmueller J.,
RA Alvarado-Balderrama L., Bauser C.A., Becker C., Birren B.W., Chen Z.,
RA Choi J., Crouch J.A., Duvick J.P., Farman M.A., Gan P., Heiman D.,
RA Henrissat B., Howard R.J., Kabbage M., Koch C., Kracher B., Kubo Y.,
RA Law A.D., Lebrun M.-H., Lee Y.-H., Miyara I., Moore N., Neumann U.,
RA Nordstroem K., Panaccione D.G., Panstruga R., Place M., Proctor R.H.,
RA Prusky D., Rech G., Reinhardt R., Rollins J.A., Rounsley S., Schardl C.L.,
RA Schwartz D.C., Shenoy N., Shirasu K., Sikhakolli U.R., Stueber K.,
RA Sukno S.A., Sweigard J.A., Takano Y., Takahara H., Trail F.,
RA van der Does H.C., Voll L.M., Will I., Young S., Zeng Q., Zhang J.,
RA Zhou S., Dickman M.B., Schulze-Lefert P., Ver Loren van Themaat E.,
RA Ma L.-J., Vaillancourt L.J.;
RT "Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered
RT by genome and transcriptome analyses.";
RL Nat. Genet. 44:1060-1065(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IMI 349063;
RA O'Connell R., Zambounis A., Thon M., Dallery J.-F.;
RT "Resequencing and annotation of the Colletotrichum higginsianum genome.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=22352720; DOI=10.1094/mpmi-12-11-0316;
RA Yoshino K., Irieda H., Sugimoto F., Yoshioka H., Okuno T., Takano Y.;
RT "Cell death of Nicotiana benthamiana is induced by secreted protein NIS1 of
RT Colletotrichum orbiculare and is suppressed by a homologue of CgDN3.";
RL Mol. Plant Microbe Interact. 25:625-636(2012).
RN [4]
RP FUNCTION.
RX PubMed=30584105; DOI=10.1073/pnas.1807297116;
RA Irieda H., Inoue Y., Mori M., Yamada K., Oshikawa Y., Saitoh H., Uemura A.,
RA Terauchi R., Kitakura S., Kosaka A., Singkaravanit-Ogawa S., Takano Y.;
RT "Conserved fungal effector suppresses PAMP-triggered immunity by targeting
RT plant immune kinases.";
RL Proc. Natl. Acad. Sci. U.S.A. 116:496-505(2019).
CC -!- FUNCTION: Secreted effector that induces necrotic lesions in Nicotiana
CC benthamiana (PubMed:22352720). Interacts with the host receptor-like
CC kinases (RLKs) BAK1/SERK3 and BKK1/SERK4, inhibits their kinase
CC activity and suppresses INF1-induced pathogen-associated molecular
CC pattern (PAMP)-triggered immunity (PTI) in N.benthamiana
CC (PubMed:30584105). Also interacts with the host receptor-like
CC cytoplasmic kinase (RLCK) BIK1 and inhibits its kinase activity,
CC thereby inhibiting PAMP-induced ROS generation (Probable). In PTI,
CC phosphorylation relaying by RLKs and RLCKs is critical for the
CC initiation of downstream signaling (Probable).
CC {ECO:0000269|PubMed:22352720, ECO:0000269|PubMed:30584105,
CC ECO:0000305|PubMed:30584105}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:30584105}. Host
CC cytoplasm {ECO:0000305|PubMed:30584105}.
CC -!- DOMAIN: The C-terminal region from residues 103 to 132 is important for
CC BAK1/SERK3-binding and the host cell death suppression.
CC {ECO:0000250|UniProtKB:N4VG36}.
CC -!- SIMILARITY: Belongs to the NIS1 effector family. {ECO:0000305}.
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DR EMBL; CACQ02008057; CCF45791.1; -; Genomic_DNA.
DR EMBL; LTAN01000005; OBR09229.1; -; Genomic_DNA.
DR RefSeq; XP_018157746.1; XM_018302968.1.
DR AlphaFoldDB; H1VZX9; -.
DR EnsemblFungi; CCF45791; CCF45791; CH063_03697.
DR GeneID; 28867075; -.
DR eggNOG; ENOG502S8NB; Eukaryota.
DR HOGENOM; CLU_138726_0_0_1; -.
DR OrthoDB; 1559161at2759; -.
DR Proteomes; UP000007174; Unassembled WGS sequence.
DR Proteomes; UP000092177; Chromosome 5.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR045469; Nis1.
DR Pfam; PF19271; Nis1; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Host cytoplasm; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..144
FT /note="Necrosis-inducing secreted protein 1"
FT /id="PRO_0000447639"
FT REGION 103..132
FT /note="BAK1/SERK3-binding"
FT /evidence="ECO:0000250|UniProtKB:N4VG36"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 144 AA; 14963 MW; 630B9A026D5EBF2E CRC64;
MQFRASIAAA AGLFALANAR IYGIAFPETV KAGDEVEAVI TTENYIQAVQ DIAIAFGIAT
EASAYPETLG NALGSFYLGP EESNTLENIT ETVTIPAGLA PGQYVVAAGL YSLYGASSSP
TLSHYNVTVT VGNVTSETYV GSQR
