NIS1_COLOR
ID NIS1_COLOR Reviewed; 162 AA.
AC N4VG36; H7CE97;
DT 31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 1.
DT 25-MAY-2022, entry version 22.
DE RecName: Full=Necrosis-inducing secreted protein 1 {ECO:0000303|PubMed:22352720};
DE AltName: Full=Secreted effector NIS1 {ECO:0000303|PubMed:22352720};
DE Flags: Precursor;
GN Name=NIS1 {ECO:0000303|PubMed:22352720}; ORFNames=Cob_01067, Cob_v001680;
OS Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS
OS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum
OS lagenarium).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC Colletotrichum orbiculare species complex.
OX NCBI_TaxID=1213857;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION, AND
RP FUNCTION.
RC STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX PubMed=22352720; DOI=10.1094/mpmi-12-11-0316;
RA Yoshino K., Irieda H., Sugimoto F., Yoshioka H., Okuno T., Takano Y.;
RT "Cell death of Nicotiana benthamiana is induced by secreted protein NIS1 of
RT Colletotrichum orbiculare and is suppressed by a homologue of CgDN3.";
RL Mol. Plant Microbe Interact. 25:625-636(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX PubMed=23252678; DOI=10.1111/nph.12085;
RA Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y.,
RA Takano Y., Kubo Y., Shirasu K.;
RT "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic
RT stage shift of Colletotrichum fungi.";
RL New Phytol. 197:1236-1249(2013).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX PubMed=30893003; DOI=10.1094/mpmi-12-18-0352-a;
RA Gan P., Tsushima A., Narusaka M., Narusaka Y., Takano Y., Kubo Y.,
RA Shirasu K.;
RT "Genome sequence resources for four phytopathogenic fungi from the
RT Colletotrichum orbiculare species complex.";
RL Mol. Plant Microbe Interact. 32:1088-1090(2019).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=24850852; DOI=10.1105/tpc.113.120600;
RA Irieda H., Maeda H., Akiyama K., Hagiwara A., Saitoh H., Uemura A.,
RA Terauchi R., Takano Y.;
RT "Colletotrichum orbiculare secretes virulence effectors to a biotrophic
RT interface at the primary hyphal neck via exocytosis coupled with SEC22-
RT mediated traffic.";
RL Plant Cell 26:2265-2281(2014).
RN [5]
RP FUNCTION, INTERACTION WITH HOST BAK1/SERK3; BKK1/SERK4 AND BIK1, AND
RP MUTAGENESIS OF TYR-125.
RX PubMed=30584105; DOI=10.1073/pnas.1807297116;
RA Irieda H., Inoue Y., Mori M., Yamada K., Oshikawa Y., Saitoh H., Uemura A.,
RA Terauchi R., Kitakura S., Kosaka A., Singkaravanit-Ogawa S., Takano Y.;
RT "Conserved fungal effector suppresses PAMP-triggered immunity by targeting
RT plant immune kinases.";
RL Proc. Natl. Acad. Sci. U.S.A. 116:496-505(2019).
CC -!- FUNCTION: Secreted effector that induces necrotic lesions in Nicotiana
CC benthamiana (PubMed:22352720). Interacts with the host receptor-like
CC kinases (RLKs) BAK1/SERK3 and BKK1/SERK4, inhibits their kinase
CC activity and suppresses INF1-induced pathogen-associated molecular
CC pattern (PAMP)-triggered immunity (PTI) in N.benthamiana
CC (PubMed:30584105). Also interacts with the host receptor-like
CC cytoplasmic kinase (RLCK) BIK1 and inhibits its kinase activity,
CC thereby inhibiting PAMP-induced ROS generation (PubMed:30584105). In
CC PTI, phosphorylation relaying by RLKs and RLCKs is critical for the
CC initiation of downstream signaling (PubMed:30584105).
CC {ECO:0000269|PubMed:22352720, ECO:0000269|PubMed:30584105}.
CC -!- SUBUNIT: Interacts with the host pattern recognition receptor (PRR)-
CC associated kinases BAK1/SERK3, BKK1/SERK4 and BIK1.
CC {ECO:0000269|PubMed:30584105}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22352720,
CC ECO:0000269|PubMed:24850852}. Host cytoplasm
CC {ECO:0000269|PubMed:24850852}. Note=Secreted, via the endoplasmic
CC reticulum-to-Golgi route and subsequent exocytosis, and accumulates in
CC a ring-like region around the neck of the biotrophic primary hyphae at
CC the pathogen-plant interface. {ECO:0000269|PubMed:24850852}.
CC -!- INDUCTION: Preferentially expressed in biotrophic invasive hyphae at
CC the post-invasive stage. {ECO:0000269|PubMed:22352720}.
CC -!- DOMAIN: The C-terminal region from residues 103 to 132 is important for
CC BAK1/SERK3-binding and the host cell death suppression.
CC {ECO:0000269|PubMed:30584105}.
CC -!- SIMILARITY: Belongs to the NIS1 effector family. {ECO:0000305}.
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DR EMBL; AB669517; BAL70334.1; -; Genomic_DNA.
DR EMBL; KB725990; ENH80006.1; -; Genomic_DNA.
DR EMBL; AMCV02000002; TDZ25263.1; -; Genomic_DNA.
DR AlphaFoldDB; N4VG36; -.
DR EnsemblFungi; ENH80006; ENH80006; Cob_01067.
DR eggNOG; ENOG502S8NB; Eukaryota.
DR HOGENOM; CLU_138726_0_0_1; -.
DR OrthoDB; 1559161at2759; -.
DR Proteomes; UP000014480; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR045469; Nis1.
DR Pfam; PF19271; Nis1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Host cytoplasm; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..162
FT /note="Necrosis-inducing secreted protein 1"
FT /id="PRO_5004123125"
FT REGION 103..132
FT /note="BAK1/SERK3-binding"
FT /evidence="ECO:0000269|PubMed:30584105"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 150
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT MUTAGEN 125
FT /note="Y->A: Specifically reduces the interaction with
FT N.benthamiana SERK3 and its paralogs but not Arabidopsis
FT BAK1."
FT /evidence="ECO:0000269|PubMed:30584105"
SQ SEQUENCE 162 AA; 16796 MW; DCC17B3BE1DBF39B CRC64;
MQFLTSLAAA ASLVSLASAR ISGIALPQTV KAGDNINAIV VTEGYIQSVQ DIAIAFGVAP
AASAYPGTLS TLLGSFYLGP EQSNVQNNIT EPITIPESLV PGEYVIAASL FSLYGASSSP
TVSNYNVTVN VGNETSTTYV RSQFYVGNSN STVCLGGYTR KI
