NIS1_MAGO7
ID NIS1_MAGO7 Reviewed; 137 AA.
AC G4MQL4;
DT 31-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=Necrosis-inducing secreted protein 1 {ECO:0000303|PubMed:22352720};
DE AltName: Full=Secreted effector NIS1 {ECO:0000303|PubMed:22352720};
DE Flags: Precursor;
GN Name=NIS1 {ECO:0000303|PubMed:22352720}; ORFNames=MGG_02347;
OS Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS fungus) (Pyricularia oryzae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX NCBI_TaxID=242507;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX PubMed=15846337; DOI=10.1038/nature03449;
RA Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL Nature 434:980-986(2005).
RN [2]
RP INDUCTION, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=22352720; DOI=10.1094/mpmi-12-11-0316;
RA Yoshino K., Irieda H., Sugimoto F., Yoshioka H., Okuno T., Takano Y.;
RT "Cell death of Nicotiana benthamiana is induced by secreted protein NIS1 of
RT Colletotrichum orbiculare and is suppressed by a homologue of CgDN3.";
RL Mol. Plant Microbe Interact. 25:625-636(2012).
RN [3]
RP FUNCTION, INTERACTION WITH HOST BAK1/SERK3; BKK1/SERK4 AND BIK1,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=30584105; DOI=10.1073/pnas.1807297116;
RA Irieda H., Inoue Y., Mori M., Yamada K., Oshikawa Y., Saitoh H., Uemura A.,
RA Terauchi R., Kitakura S., Kosaka A., Singkaravanit-Ogawa S., Takano Y.;
RT "Conserved fungal effector suppresses PAMP-triggered immunity by targeting
RT plant immune kinases.";
RL Proc. Natl. Acad. Sci. U.S.A. 116:496-505(2019).
CC -!- FUNCTION: Secreted effector that induces necrotic lesions in Nicotiana
CC benthamiana (PubMed:22352720). Interacts with the rice receptor-like
CC kinases (RLKs) BAK1/SERK3 and BKK1/SERK4, inhibits their kinase
CC activity and suppresses INF1-induced pathogen-associated molecular
CC pattern (PAMP)-triggered immunity (PTI) in N.benthamiana
CC (PubMed:30584105). Also interacts with the rice receptor-like
CC cytoplasmic kinase (RLCK) BIK1 and inhibits its kinase activity,
CC thereby inhibiting PAMP-induced ROS generation (PubMed:30584105). In
CC PTI, phosphorylation relaying by RLKs and RLCKs is critical for the
CC initiation of downstream signaling (PubMed:30584105). Suppresses
CC R3a/Avr3a-dependent hypersensitive response (HR) cell deat and thus may
CC have additional targets, probably belonging to a kinase family that
CC regulates Avr3a effector-triggered immunity (ETI) (PubMed:30584105).
CC {ECO:0000269|PubMed:22352720, ECO:0000269|PubMed:30584105}.
CC -!- SUBUNIT: Interacts with the host pattern recognition receptor (PRR)-
CC associated kinases BAK1/SERK3, BKK1/SERK4 and BIK1.
CC {ECO:0000269|PubMed:30584105}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30584105}. Host
CC cytoplasm {ECO:0000269|PubMed:30584105}.
CC -!- DOMAIN: The C-terminal region from residues 107 to 136 is important for
CC BAK1/SERK3-binding and the host cell death suppression.
CC {ECO:0000250|UniProtKB:N4VG36}.
CC -!- DISRUPTION PHENOTYPE: Severely compromised the virulence of M.oryzae on
CC rice and barley. {ECO:0000269|PubMed:30584105}.
CC -!- SIMILARITY: Belongs to the NIS1 effector family. {ECO:0000305}.
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DR EMBL; CM001231; EHA56504.1; -; Genomic_DNA.
DR RefSeq; XP_003709116.1; XM_003709068.1.
DR AlphaFoldDB; G4MQL4; -.
DR EnsemblFungi; MGG_02347T0; MGG_02347T0; MGG_02347.
DR GeneID; 2681444; -.
DR KEGG; mgr:MGG_02347; -.
DR VEuPathDB; FungiDB:MGG_02347; -.
DR eggNOG; ENOG502T0WH; Eukaryota.
DR HOGENOM; CLU_1844997_0_0_1; -.
DR InParanoid; G4MQL4; -.
DR OMA; WEDYSIV; -.
DR OrthoDB; 1491199at2759; -.
DR Proteomes; UP000009058; Chromosome 1.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR045469; Nis1.
DR Pfam; PF19271; Nis1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Host cytoplasm; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..137
FT /note="Necrosis-inducing secreted protein 1"
FT /id="PRO_5003465997"
FT REGION 107..136
FT /note="BAK1/SERK3-binding"
FT /evidence="ECO:0000250|UniProtKB:N4VG36"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 137 AA; 14195 MW; 26D24E8BE61B75B4 CRC64;
MQLLATTLTF ASGAAAYINS MSAPASVQRG QAFTAQLNSS IYVQNYDDFG VVWGLAPPNL
NTSACVGCVG RRIGYTNLFG DKADVQVPPS GTVGVQVTVP ADQAPGEYLL IAGASYLVGA
SGVTGFNYFN TTVQVCE
