NISB_LACLL
ID NISB_LACLL Reviewed; 993 AA.
AC P20103;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 3.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Nisin biosynthesis protein NisB;
GN Name=nisB;
OS Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1360;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=6F3;
RX PubMed=1482192; DOI=10.1128/aem.58.11.3730-3743.1992;
RA Engelke G., Gutowski-Eckel Z., Hammelmann M., Entian K.-D.;
RT "Biosynthesis of the lantibiotic nisin: genomic organization and membrane
RT localization of the NisB protein.";
RL Appl. Environ. Microbiol. 58:3730-3743(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NIZO R5;
RX PubMed=7689965; DOI=10.1111/j.1432-1033.1993.tb18143.x;
RA Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M.;
RT "Characterization of the nisin gene cluster nisABTCIPR of Lactococcus
RT lactis. Requirement of expression of the nisA and nisI genes for
RT development of immunity.";
RL Eur. J. Biochem. 216:281-291(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-852.
RC STRAIN=ATCC 11454 / DSM 20729 / LMG 7930 / NCDO 496 / NCIMB 8586 / Berridge
RC X 13;
RX PubMed=1905517; DOI=10.1128/aem.57.4.1181-1188.1991;
RA Steen M.T., Chung Y.J., Hansen J.N.;
RT "Characterization of the nisin gene as part of a polycistronic operon in
RT the chromosome of Lactococcus lactis ATCC 11454.";
RL Appl. Environ. Microbiol. 57:1181-1188(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
RC STRAIN=ATCC 11454 / DSM 20729 / LMG 7930 / NCDO 496 / NCIMB 8586 / Berridge
RC X 13;
RX PubMed=3141403; DOI=10.1016/s0021-9258(18)37587-2;
RA Buchman G.W., Banerjee S., Hansen J.N.;
RT "Structure, expression, and evolution of a gene encoding the precursor of
RT nisin, a small protein antibiotic.";
RL J. Biol. Chem. 263:16260-16266(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
RC STRAIN=CIP 103449 / JCM 7638;
RA Araya T., Ishibashi N., Shimamura S.;
RT "Genetic evidence that Lactococcus lactis JCM7638 produces a mutated form
RT of nisin.";
RL J. Gen. Appl. Microbiol. 38:271-278(1992).
CC -!- FUNCTION: Involved in the post-translational modification of the
CC lantibiotic nisin.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein;
CC Cytoplasmic side. Note=Possibly associated with, and anchored to, the
CC cytoplasmic side of the membrane.
CC -!- SIMILARITY: To B.subtilis SpaB and S.epidermidis EpiB. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X68307; CAA48381.1; -; Genomic_DNA.
DR EMBL; L16226; AAA25190.1; -; Genomic_DNA.
DR EMBL; M65089; AAA73039.1; -; Genomic_DNA.
DR EMBL; J04057; AAA88607.1; -; Genomic_DNA.
DR EMBL; D10768; BAA01599.1; -; Genomic_DNA.
DR PIR; S36735; C31915.
DR PDB; 4WD9; X-ray; 2.90 A; A/B=1-993.
DR PDB; 6M7Y; X-ray; 2.79 A; A/B=2-993.
DR PDBsum; 4WD9; -.
DR PDBsum; 6M7Y; -.
DR AlphaFoldDB; P20103; -.
DR SMR; P20103; -.
DR DIP; DIP-61450N; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR006827; Lant_deHydtase_N.
DR InterPro; IPR023809; Thiopep_bacteriocin_synth_dom.
DR Pfam; PF14028; Lant_dehydr_C; 1.
DR Pfam; PF04738; Lant_dehydr_N; 1.
DR TIGRFAMs; TIGR03891; thiopep_ocin; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..993
FT /note="Nisin biosynthesis protein NisB"
FT /id="PRO_0000096863"
FT TRANSMEM 838..851
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 19
FT /note="C -> S (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 656
FT /note="K -> E (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 841..852
FT /note="CADSKIIPNLLT -> VPILKLFQICLH (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 895
FT /note="T -> P (in Ref. 2; AAA25190)"
FT /evidence="ECO:0000305"
FT STRAND 11..18
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 20..22
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 29..36
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 40..49
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 51..61
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 67..69
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 70..86
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 92..94
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 95..102
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 115..120
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 122..135
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 141..144
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 148..151
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 154..159
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 168..172
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 175..183
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 185..187
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 191..199
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 200..202
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 204..206
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 207..219
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 226..228
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 230..233
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 237..247
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 256..267
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 272..274
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 276..289
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 296..301
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 310..325
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 326..329
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 334..346
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 348..353
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 354..357
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 360..362
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 385..387
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 389..404
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 411..414
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 415..420
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 423..425
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 432..441
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 444..456
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 458..465
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 466..468
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 470..489
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 493..499
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 504..508
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 516..525
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 527..530
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 533..535
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 536..540
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 546..550
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 551..554
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 555..560
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 567..569
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 572..580
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 584..587
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 590..595
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 599..601
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 604..606
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 609..612
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 615..619
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 620..622
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 629..635
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 640..645
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 650..656
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 658..674
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 677..682
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 687..694
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 699..705
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 726..729
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 735..744
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 746..755
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 757..766
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 770..776
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 778..780
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 782..788
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 792..808
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 811..817
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 824..828
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 830..855
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 857..859
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 863..878
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 879..881
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 883..891
FT /evidence="ECO:0007829|PDB:6M7Y"
FT TURN 921..924
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 926..944
FT /evidence="ECO:0007829|PDB:6M7Y"
FT HELIX 949..967
FT /evidence="ECO:0007829|PDB:6M7Y"
FT STRAND 973..975
FT /evidence="ECO:0007829|PDB:4WD9"
FT HELIX 976..987
FT /evidence="ECO:0007829|PDB:6M7Y"
SQ SEQUENCE 993 AA; 117502 MW; 0027053BEAE71E2D CRC64;
MIKSSFKAQP FLVRNTILCP NDKRSFTEYT QVIETVSKNK VFLEQLLLAN PKLYDVMQKY
NAGLLKKKRV KKLFESIYKY YKRSYLRSTP FGLFSETSIG VFSKSSQYKL MGKTTKGIRL
DTQWLIRLVH KMEVDFSKKL SFTRNNANYK FGDRVFQVYT INSSELEEVN IKYTNVYQII
SEFCENDYQK YEDICETVTL CYGDEYRELS EQYLGSLIVN HYLISNLQKD LLSDFSWNTF
LTKVEAIDED KKYIIPLKKV QKFIQEYSEI EIGEGIEKLK EIYQEMSQIL ENDNYIQIDL
ISDSEINFDV KQKQQLEHLA EFLGNTTKSV RRTYLDDYKD KFIEKYGVDQ EVQITELFDS
TFGIGAPYNY NHPRNDFYES EPSTLYYSEE EREKYLSMYV EAVKNHNVIN LDDLESHYQK
MDLEKKSELQ GLELFLNLAK EYEKDIFILG DIVGNNNLGG ASGRFSALSP ELTSYHRTIV
DSVERENENK EITSCEIVFL PENIRHANVM HTSIMRRKVL PFFTSTSHNE VLLTNIYIGI
DEKEKFYARD ISTQEVLKFY ITSMYNKTLF SNELRFLYEI SLDDKFGNLP WELIYRDFDY
IPRLVFDEIV ISPAKWKIWG RDVNSKMTIR ELIQSKEIPK EFYIVNGDNK VYLSQKNPLD
MEILESAIKK SSKRKDFIEL QEYFEDENII NKGEKGRVAD VVVPFIRTRA LGNEGRAFIR
EKRVSVERRE KLPFNEWLYL KLYISINRQN EFLLSYLPDI QKIVANLGGN LFFLRYTDPK
PHIRLRIKCS DLFLAYGSIL EILKRSRKNR IMSTFDISIY DQEVERYGGF DTLELSEAIF
CADSKIIPNL LTLIKDTNND WKVDDVSILV NYLYLKCFFQ NDNKKILNFL NLVSTKKVKE
NVNEKIEHYL KLLKVNNLGD QIFYDKNFKE LKHAIKNLFL KMIAQDFELQ KVYSIIDSII
HVHNNRLIGI ERDKEKLIYY TLQRLFVSEE YMK
