ID   NISI_LACLL              Reviewed;         245 AA.
AC   P42708;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Nisin immunity protein;
DE   Flags: Precursor;
GN   Name=nisI;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NIZO R5;
RX   PubMed=7689965; DOI=10.1111/j.1432-1033.1993.tb18143.x;
RA   Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M.;
RT   "Characterization of the nisin gene cluster nisABTCIPR of Lactococcus
RT   lactis. Requirement of expression of the nisA and nisI genes for
RT   development of immunity.";
RL   Eur. J. Biochem. 216:281-291(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=6F3;
RX   PubMed=8161176; DOI=10.1128/aem.60.3.814-825.1994;
RA   Engelke G., Gutowski-Eckel Z., Kiesau P., Siegers K., Hammelmann M.,
RA   Entian K.-D.;
RT   "Regulation of nisin biosynthesis and immunity in Lactococcus lactis 6F3.";
RL   Appl. Environ. Microbiol. 60:814-825(1994).
CC   -!- FUNCTION: Involved in immunity against exogenously supplied nisin.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; L16226; AAA25193.1; -; Genomic_DNA.
DR   EMBL; X76884; CAA54209.1; -; Genomic_DNA.
DR   PIR; S36738; S36738.
DR   RefSeq; WP_014570409.1; NZ_ML956318.1.
DR   PDB; 2N2E; NMR; -; A=117-245.
DR   PDB; 2N32; NMR; -; A=21-129.
DR   PDB; 5XHB; X-ray; 1.90 A; A=22-245.
DR   PDBsum; 2N2E; -.
DR   PDBsum; 2N32; -.
DR   PDBsum; 5XHB; -.
DR   AlphaFoldDB; P42708; -.
DR   BMRB; P42708; -.
DR   SMR; P42708; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR   InterPro; IPR040876; Spa1_C.
DR   Pfam; PF18218; Spa1_C; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Bacteriocin immunity; Cell membrane; Lipoprotein; Membrane;
KW   Palmitate; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..245
FT                   /note="Nisin immunity protein"
FT                   /id="PRO_0000017152"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   STRAND          35..40
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          43..51
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   HELIX           54..56
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          57..71
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   TURN            72..74
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          77..80
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          85..96
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          101..105
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          108..114
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   HELIX           115..117
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   TURN            118..120
FT                   /evidence="ECO:0007829|PDB:2N32"
FT   HELIX           126..128
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          141..143
FT                   /evidence="ECO:0007829|PDB:2N2E"
FT   STRAND          149..152
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          155..163
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   TURN            166..168
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          169..183
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   TURN            184..186
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   HELIX           192..194
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          200..202
FT                   /evidence="ECO:0007829|PDB:2N2E"
FT   STRAND          209..220
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   TURN            225..227
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          228..233
FT                   /evidence="ECO:0007829|PDB:5XHB"
FT   STRAND          236..242
FT                   /evidence="ECO:0007829|PDB:5XHB"
SQ   SEQUENCE   245 AA;  27837 MW;  72DCE887DBB0E30C CRC64;
     MRRYLILIVA LIGITGLSGC YQTSHKKVRF DEGSYTNFIY DNKSYFVTDK EIPQENVNNS
     KVKFYKLLIV DMKSEKLLSS SNKNSVTLVL NNIYEASDKS LCMGINDRYY KILPESDKGA
     VKALRLQNFD VTSDISDDNF VIDKNDSRKI DYMGNIYSIS DTTVSDEELG EYQDVLAEVR
     VFDSVSGKSI PRSEWGRIDK DGSNSKQSRT EWDYGEIHSI RGKSLTEAFA VEINDDFKLA
     TKVGN