NIST_LACLL
ID NIST_LACLL Reviewed; 600 AA.
AC Q03203;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Nisin transport ATP-binding protein NisT;
GN Name=nisT;
OS Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1360;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=6F3;
RX PubMed=1482192; DOI=10.1128/aem.58.11.3730-3743.1992;
RA Engelke G., Gutowski-Eckel Z., Hammelmann M., Entian K.-D.;
RT "Biosynthesis of the lantibiotic nisin: genomic organization and membrane
RT localization of the NisB protein.";
RL Appl. Environ. Microbiol. 58:3730-3743(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NIZO R5;
RX PubMed=7689965; DOI=10.1111/j.1432-1033.1993.tb18143.x;
RA Kuipers O.P., Beerthuyzen M.M., Siezen R.J., de Vos W.M.;
RT "Characterization of the nisin gene cluster nisABTCIPR of Lactococcus
RT lactis. Requirement of expression of the nisA and nisI genes for
RT development of immunity.";
RL Eur. J. Biochem. 216:281-291(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-7.
RC STRAIN=ATCC 11454 / DSM 20729 / LMG 7930 / NCDO 496 / NCIMB 8586 / Berridge
RC X 13;
RX PubMed=1905517; DOI=10.1128/aem.57.4.1181-1188.1991;
RA Steen M.T., Chung Y.J., Hansen J.N.;
RT "Characterization of the nisin gene as part of a polycistronic operon in
RT the chromosome of Lactococcus lactis ATCC 11454.";
RL Appl. Environ. Microbiol. 57:1181-1188(1991).
CC -!- FUNCTION: Probably implicated in the export process of the lantibiotic
CC nisin.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nisin exporter
CC (TC 3.A.1.111.3) family. {ECO:0000305}.
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DR EMBL; X68307; CAA48382.1; -; Genomic_DNA.
DR EMBL; L16226; AAA25191.1; -; Genomic_DNA.
DR EMBL; M65089; AAA73040.1; -; Genomic_DNA.
DR PIR; E48951; E48951.
DR PIR; S36736; S36736.
DR RefSeq; WP_014570407.1; NZ_ML956318.1.
DR AlphaFoldDB; Q03203; -.
DR SMR; Q03203; -.
DR TCDB; 3.A.1.111.3; the atp-binding cassette (abc) superfamily.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1560.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039421; Type_1_exporter.
DR PANTHER; PTHR24221; PTHR24221; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF90123; SSF90123; 1.
DR PROSITE; PS50929; ABC_TM1F; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Bacteriocin transport; Cell membrane; Membrane;
KW Nucleotide-binding; Protein transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..600
FT /note="Nisin transport ATP-binding protein NisT"
FT /id="PRO_0000092632"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 34..317
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 352..592
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 386..393
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CONFLICT 14
FT /note="Y -> N (in Ref. 2; AAA25191)"
FT /evidence="ECO:0000305"
FT CONFLICT 151
FT /note="E -> V (in Ref. 2; AAA25191)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 600 AA; 69210 MW; AE92F317BD9045A5 CRC64;
MDEVKEFTSK QFFYTLLTLP STLKLIFQLE KRYAIYLIVL NAITAFVPLA SLFIYQDLIN
SVLGSGRHLI NIIIIYFIVQ VITTVLGQLE SYVSGKFDMR LSYSINMRLM RTTSSLELSD
YEQADMYNII EKVTQDSTYK PFQLFNAIIV ELSSFISLLS SLFFIGTWNI GVAILLLIVP
VLSLVLFLRV GQLEFLIQWQ RASSERETWY IVYLLTHDFS FKEIKLNNIS NYFIHKFGKL
KKGFINQDLA IARKKTYFNI FLDFILNLIN ILTIFAMILS VRAGKLLIGN LVSLIQAISK
INTYSQTMIQ NIYIIYNTSL FMEQLFEFLK RESVVHKKIE DTEICNQHIG TVKVINLSYV
YPNSNAFALK NINLSFEKGE LTAIVGKNGS GKSTLVKIIS GLYQPTMGII QYDKMRSSLM
PEEFYQKNIS VLFQDFVKYE LTIRENIGLS DLSSQWEDEK IIKVLDNLGL DFLKTNNQYV
LDTQLGNWFQ EGHQLSGGQW QKIALARTFF KKASIYILDE PSAALDPVAE KEIFDYFVAL
SENNISIFIS HSLNAARKAN KIVVMKDGQV EDVGSHDVLL RRCQYYQELY YSEQYEDNDE
