ID   NIT2_DICDI              Reviewed;         328 AA.
AC   Q54JM9;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Nitrilase homolog 2;
DE            EC=3.5.-.-;
GN   Name=nit2; ORFNames=DDB_G0287939;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       NIT1/NIT2 family. {ECO:0000305}.
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DR   EMBL; AAFI02000105; EAL63476.1; -; Genomic_DNA.
DR   RefSeq; XP_636983.1; XM_631891.1.
DR   AlphaFoldDB; Q54JM9; -.
DR   SMR; Q54JM9; -.
DR   STRING; 44689.DDB0302493; -.
DR   PaxDb; Q54JM9; -.
DR   EnsemblProtists; EAL63476; EAL63476; DDB_G0287939.
DR   GeneID; 8626377; -.
DR   KEGG; ddi:DDB_G0287939; -.
DR   dictyBase; DDB_G0287939; nit2.
DR   eggNOG; KOG0806; Eukaryota.
DR   HOGENOM; CLU_030130_1_0_1; -.
DR   InParanoid; Q54JM9; -.
DR   OMA; GNTYRES; -.
DR   PhylomeDB; Q54JM9; -.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   PRO; PR:Q54JM9; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0050152; F:omega-amidase activity; IBA:GO_Central.
DR   GO; GO:0006528; P:asparagine metabolic process; IBA:GO_Central.
DR   GO; GO:0006541; P:glutamine metabolic process; IBA:GO_Central.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IBA:GO_Central.
DR   CDD; cd07572; nit; 1.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR045254; Nit1/2_C-N_Hydrolase.
DR   InterPro; IPR001110; UPF0012_CS.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS01227; UPF0012; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..328
FT                   /note="Nitrilase homolog 2"
FT                   /id="PRO_0000332973"
FT   DOMAIN          53..299
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        92
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        163
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        204
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ   SEQUENCE   328 AA;  36945 MW;  80EA9A1BA2537BEB CRC64;
     MYRGLINSFK NTSKFSTSLF NNNNNCSTTT KSFTHISQQL RKVHLMADKE KVFKFAGIQL
     LCGDNKEENV QNAIKHIDEA AKNGAKLISL PECFNSPYST STFEKYSETE DGETVKKLSE
     AAKRNQIFLV GGSIPEIDKA TGKIYNTCFI FNDKGEVVKK HRKIHLFDID VPNKIRFKES
     ETLTPGDSFS VVDIGYCKIG VAICYDIRFP ELAMLYSKMG AKFLIYPGAF NMVTGPAHWE
     LLQRGRAVDN QVFVAAISPA RNPSSTYQAW GHSTIVNSWG TILATTDEHQ SIIYSDIDLN
     TLNETRSSIP IYSQKRDDLY KLDSIKKQ