NIT2_NEUCR
ID NIT2_NEUCR Reviewed; 1036 AA.
AC P19212; Q7SCS7;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Nitrogen catabolic enzyme regulatory protein;
DE AltName: Full=Nitrogen regulatory protein 2;
DE Short=NIT2;
GN Name=nit-2; Synonyms=amr, pink; ORFNames=NCU09068;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=2137552; DOI=10.1128/mcb.10.3.1056-1065.1990;
RA Fu Y.-H., Marzluf G.A.;
RT "nit-2, the major nitrogen regulatory gene of Neurospora crassa, encodes a
RT protein with a putative zinc finger DNA-binding domain.";
RL Mol. Cell. Biol. 10:1056-1065(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [3]
RP MUTAGENESIS.
RX PubMed=2150539; DOI=10.1111/j.1365-2958.1990.tb02033.x;
RA Fu Y.-H., Marzluf G.A.;
RT "Site-directed mutagenesis of the 'zinc finger' DNA-binding domain of the
RT nitrogen-regulatory protein NIT2 of Neurospora.";
RL Mol. Microbiol. 4:1847-1852(1990).
RN [4]
RP FUNCTION, AND INTERACTION WITH NMR-1.
RX PubMed=7612627; DOI=10.1021/bi00027a038;
RA Xiao X., Fu Y.H., Marzluf G.A.;
RT "The negative-acting NMR regulatory protein of Neurospora crassa binds to
RT and inhibits the DNA-binding activity of the positive-acting nitrogen
RT regulatory protein NIT2.";
RL Biochemistry 34:8861-8868(1995).
CC -!- FUNCTION: Major nitrogen regulatory protein. During conditions of
CC nitrogen limitation it turns on the expression of genes for enzymes
CC which are required for the use of a variety of secondary nitrogen
CC sources, including nitrates, purines, amino acids, and proteins.
CC {ECO:0000269|PubMed:7612627}.
CC -!- SUBUNIT: Interacts with nmr. {ECO:0000269|PubMed:7612627}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- INDUCTION: By lack of a primary nitrogen source.
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DR EMBL; M33956; AAB03891.1; -; Genomic_DNA.
DR EMBL; CM002236; EAA34560.3; -; Genomic_DNA.
DR PIR; A34755; A34755.
DR RefSeq; XP_963796.3; XM_958703.3.
DR PDB; 5VQI; X-ray; 2.50 A; A/C=915-927.
DR PDB; 6P6A; X-ray; 2.15 A; A/C=915-927.
DR PDBsum; 5VQI; -.
DR PDBsum; 6P6A; -.
DR AlphaFoldDB; P19212; -.
DR SMR; P19212; -.
DR STRING; 5141.EFNCRP00000008682; -.
DR EnsemblFungi; EAA34560; EAA34560; NCU09068.
DR GeneID; 3879945; -.
DR KEGG; ncr:NCU09068; -.
DR VEuPathDB; FungiDB:NCU09068; -.
DR HOGENOM; CLU_903421_0_0_1; -.
DR InParanoid; P19212; -.
DR Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR013860; AreA_GATA.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF08550; DUF1752; 1.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Metal-binding; Nitrate assimilation;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..1036
FT /note="Nitrogen catabolic enzyme regulatory protein"
FT /id="PRO_0000083478"
FT REPEAT 49..55
FT /note="1"
FT REPEAT 87..92
FT /note="2"
FT REPEAT 105..110
FT /note="3"
FT ZN_FING 743..767
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 49..110
FT /note="3 X approximate repeats"
FT REGION 207..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 590..743
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 792..976
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1000..1028
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..240
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..342
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 630..679
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 686..711
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 718..743
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 797..886
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 928..976
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1007..1022
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 743..746
FT /note="CTNC->STNG: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:2150539"
FT MUTAGEN 755..756
FT /note="RR->GG: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:2150539"
FT MUTAGEN 765..766
FT /note="NA->DV: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:2150539"
FT MUTAGEN 768..769
FT /note="GL->DV: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:2150539"
FT MUTAGEN 789..790
FT /note="KR->NS: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:2150539"
FT CONFLICT 83
FT /note="D -> E (in Ref. 1; EAA34560)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1036 AA; 109296 MW; 84971B35DE109B66 CRC64;
MAASTTTPTA TTRPFFTMNP TTTEHDFRFP RRPGDSMAGT GLGGAAMSSS SANNNHNQHH
PMSAFNHHHH HNAAGSARGR DSDGRPSSSN NNTSNGFVAN INHQSSSNNN ISKNIPPPTS
DYHTQSASNG AAAYDLLRSS AFPPFQDGLA GMTQSPDEMQ KQDPLATQVW KYFAKTKLAL
PNQERMENLT WRMMAKPLQT YRRQMETDRT HRFSESAPQK STSGIARLRK SSEQTQSQGS
DLMNLDDFIN GENISTPAGL SLAPSPETSS KMADDRTAHH STASAIPIKA RKDQQSQHMI
PQSVPAALHH PRMQTEFGYL PRHLRKTSID ETSKRNPNRK RPADFSPHVS AVTPSYVTNG
LDADTDLHDY SLDHTSHDGL PPQTAPSSVP YALDTVGLDA DTFITSAGPF QQNFSFSPST
SPMVSHDPFT AMFGPNNSSM HSGPINGNNF YSPPASAFQS TASTPHPMNE GGDNFYFGVD
MRRARQQPYQ PGNHGMGNAM AHQFPYAGNG NMMFPASSAG QDPTPSFAAP NSFSGHIDPT
QVFHNEQAVR SPGMSVLQDS LFTFGAESDG DEEDGGAFAD RNLSISHDFS SQGMEEPAFD
SPSMGWDPSL PGNFSTQAAR YPGGPPRKQV TIGATTTDYV DNTGEWDGSG LPRSQSQSFR
QSDLRKGKMS RTASTPGLSA RMNPFERLAQ SASHSPPADV GRSSGLSSVP ASRPSSPPPG
AKQGSTTNLQ GAAGNSTDTP TTCTNCFTQT TPLWRRNPDG QPLCNACGLF LKLHGVVRPL
SLKTDVIKKR NRGSGASLPV GGTSTRSKKN ASMSAAARKN STLSITSNAN NQPPAQVATP
PAQQQVRASS VNESESPASG PASGGNTAGS TPTSYHGSTG STSGAVGGKS VIPIASAPPK
SAPGPGAGSM SRRDTISSKR QRRHSKSAGS DQPVSAGAVS SSGMDVDSPA NSTGSNETMP
TFNPGGAFSG LPPTTQSSLG FGNGYINTPR PMVGPGGMMG MPNGQAGQMM GASSSSGPGS
GPSRTGAEWE WLTMSL
