ID   NIT4_NEUCR              Reviewed;        1090 AA.
AC   P28349; Q7RVG9;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   25-MAY-2022, entry version 125.
DE   RecName: Full=Nitrogen assimilation transcription factor nit-4;
GN   Name=nit-4; ORFNames=NCU08294;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1840634; DOI=10.1128/mcb.11.11.5735-5745.1991;
RA   Yuan G.-F., Fu Y.-H., Marzluf G.A.;
RT   "nit-4, a pathway-specific regulatory gene of Neurospora crassa, encodes a
RT   protein with a putative binuclear zinc DNA-binding domain.";
RL   Mol. Cell. Biol. 11:5735-5745(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1531376; DOI=10.1111/j.1365-2958.1992.tb00838.x;
RA   Yuan G.-F., Marzluf G.A.;
RT   "Molecular characterization of mutations of nit-4, the pathway-specific
RT   regulatory gene which controls nitrate assimilation in Neurospora crassa.";
RL   Mol. Microbiol. 6:67-73(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Pathway-specific regulatory gene of nitrate assimilation; it
CC       activates the transcription of the genes for nitrate and nitrite
CC       reductases.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DOMAIN: The glutamine-rich domain might function in activating gene
CC       expression.
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DR   EMBL; M80368; AAA33602.1; -; Genomic_DNA.
DR   EMBL; CM002239; EAA33271.3; -; Genomic_DNA.
DR   PIR; A41696; A41696.
DR   RefSeq; XP_962507.3; XM_957414.3.
DR   AlphaFoldDB; P28349; -.
DR   STRING; 5141.EFNCRP00000008391; -.
DR   EnsemblFungi; EAA33271; EAA33271; NCU08294.
DR   GeneID; 3878673; -.
DR   KEGG; ncr:NCU08294; -.
DR   VEuPathDB; FungiDB:NCU08294; -.
DR   HOGENOM; CLU_007003_6_0_1; -.
DR   InParanoid; P28349; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   4: Predicted;
KW   Activator; DNA-binding; Metal-binding; Nitrate assimilation; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..1090
FT                   /note="Nitrogen assimilation transcription factor nit-4"
FT                   /id="PRO_0000114960"
FT   DNA_BIND        53..81
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          145..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          666..689
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          773..798
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          825..875
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          936..999
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1033..1053
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        145..166
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        784..798
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        98
FT                   /note="K -> KP (in Ref. 1; AAA33602)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        276..277
FT                   /note="KP -> NA (in Ref. 1; AAA33602 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        467
FT                   /note="L -> S (in Ref. 1; AAA33602)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1090 AA;  120284 MW;  CD5AC9115CF99D2D CRC64;
     MNSSDVQMMS SQDAPGSAGL APDNIASSLP SKKKSRRGAD PTNQKRRCVS TACIACRRRK
     SKCDGALPSC AACASVYGTE CIYDPNSDHR RKGVYREKND SMKAQNATLQ ILIEAILNAS
     EEDVIDIVRR IRTCDDLDEV AESIRRDEKN ATATNDNDDS DEPTQPGRDD ATSQAVEGER
     DLARKMGELR IENGSVRFIG GTSHLIYLSE PTDASEEPEL ETRLSTCDEN PITTWTEVTK
     NPQLIIHLVN MYFNWHYPYF TTLSRSLFYR DFIKGKPAGQ PRSTVYCSSL LVNAMLALGC
     HFTSVDGAFA VPGDSRTKGD HFFAEAKRLI VQNDEYEKPR LTTVQALALM SVREAGCGRE
     AKGWVYSGMS FRMAQDIGLN LDIGSLDEKE VDARRITFWG CFVFDKCWSN YLGRLPQLPK
     NTYNVPKYDV FPDEDAELWS PYTDAGFDQS CKQPSRTRAI GLQLSKLCEI SSDLLLFFYH
     PSHIGRSSGK SAELKKLSEL HRRLEDWRTE LPKEFEPKDG QLPNVILMHM FYHLQYIHLF
     RPFLKYTKEA SPLEKVQPRR ICTTNANSIS KLMRLYKKLY NLRQICNIAV YMLHSACTIH
     MLNLPEKTAR RDITHGVRQL EEMAEDWPCA RRTLGIISVL ARKWNVELPE EAAIVLKRTD
     EKYGMFSTSE VPSPNRTAPS LAPSSPAPPY TPEASLLFST TAAAVLEQQP YSPMTLYSHP
     TPPHLGPESI SDPGMSPNIV TFSDLPDPSA PIIPQQQQNM QAISSLSQNN MLHQHHHHLQ
     NQHQPQQPHH NHMTYQQQQH NLLTHPVSAS SMSMSDTLAT ITAWGIPTSS PGNNNNNNIV
     SQHPHHQKQP QQQQQPQAQR YPTVGSVGTN TVKPPAAATQ TFTPAQLHAN NLATATRSTA
     SNHKSVGRHV SPSSIYAIDG QDWYLKDGVT WQQGFQGWDL EGGGAGTATS TGGIGDGGGP
     TGGAGDSMAR LAPRGNIGGG GGGGGGSTGQ RQQQQQRQQQ QQQQQQQQQQ QQQQQQQQQQ
     QQQQEANMFA YHHGAERGGG GIESTGMGMT TVGGGGGGFD PIGGSGLLDD LVGLDELGSL
     DGLGHLPGLD