ID   NIXA_HELPJ              Reviewed;         331 AA.
AC   Q9ZM74;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=High-affinity nickel-transport protein NixA;
GN   Name=nixA; OrderedLocusNames=jhp_0348;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- FUNCTION: High-affinity nickel intake protein. Imports nickel ions in
CC       an energy-dependent fashion. Necessary for the expression of
CC       catalytically active urease.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC       {ECO:0000305}.
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DR   EMBL; AE001439; AAD05929.1; -; Genomic_DNA.
DR   PIR; F71943; F71943.
DR   RefSeq; WP_000780409.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZM74; -.
DR   STRING; 85963.jhp_0348; -.
DR   EnsemblBacteria; AAD05929; AAD05929; jhp_0348.
DR   KEGG; hpj:jhp_0348; -.
DR   PATRIC; fig|85963.30.peg.664; -.
DR   eggNOG; COG3376; Bacteria.
DR   OMA; VSKSWHI; -.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR004688; Ni/Co_transpt.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   PANTHER; PTHR31611; PTHR31611; 1.
DR   Pfam; PF03824; NicO; 1.
DR   TIGRFAMs; TIGR00802; nico; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Nickel; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..331
FT                   /note="High-affinity nickel-transport protein NixA"
FT                   /id="PRO_0000194006"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   331 AA;  36991 MW;  583BE197DC350C22 CRC64;
     MKLWFPYFLA IVFLHALGLA LLFMANNASF YAAASMAYML GAKHAFDADH IACIDNTIRK
     LTQQGKNAYG VGFYFSMGHS SVVILMTIIS AFAIAWAKEH TPMLEEIGGV VGTLVSGLFL
     LIIGLLNAII LIDLLKIFKK SHSNESLSRQ QNEEIERLLT SRGLLNRFFK PLFNFVSKSW
     HIYPVGFLFG LGFDTASEIA LLALSSSAIK VSVVGMLSLP ILFAAGMSLF DTLDGAFMLK
     AYDWAFKTPL RKIYYNISIT ALSVFIALFI GLIELFQVIS EKLHLKFENR LLSTLQSLEF
     TDLGYYLVGL FVIAFLGSFF LWKIKFSKLE S