ID   NIXA_STAA8              Reviewed;         338 AA.
AC   Q2FUR9;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Nickel transporter NixA {ECO:0000305};
GN   Name=nixA {ECO:0000303|PubMed:20662775};
GN   OrderedLocusNames=SAOUHSC_03033 {ECO:0000312|EMBL:ABD32018.1};
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   FUNCTION IN NICKEL TRANSPORT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=RN6390;
RX   PubMed=20662775; DOI=10.1111/j.1365-2958.2010.07287.x;
RA   Hiron A., Posteraro B., Carriere M., Remy L., Delporte C., La Sorda M.,
RA   Sanguinetti M., Juillard V., Borezee-Durant E.;
RT   "A nickel ABC-transporter of Staphylococcus aureus is involved in urinary
RT   tract infection.";
RL   Mol. Microbiol. 77:1246-1260(2010).
CC   -!- FUNCTION: Secondary nickel transporter. Required for full urease
CC       activity. {ECO:0000269|PubMed:20662775}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Transiently induced in response to mild-acid conditions.
CC       {ECO:0000269|PubMed:20662775}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant is impaired in its capacity to
CC       accumulate nickel. Nickel accumulation and urease activity are almost
CC       completely abolished in a nixA-nikA double mutant.
CC       {ECO:0000269|PubMed:20662775}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC       {ECO:0000305}.
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DR   EMBL; CP000253; ABD32018.1; -; Genomic_DNA.
DR   RefSeq; WP_000215642.1; NZ_LS483365.1.
DR   RefSeq; YP_501481.1; NC_007795.1.
DR   AlphaFoldDB; Q2FUR9; -.
DR   STRING; 1280.SAXN108_2971; -.
DR   EnsemblBacteria; ABD32018; ABD32018; SAOUHSC_03033.
DR   GeneID; 3921299; -.
DR   KEGG; sao:SAOUHSC_03033; -.
DR   PATRIC; fig|93061.5.peg.2740; -.
DR   eggNOG; COG3376; Bacteria.
DR   HOGENOM; CLU_036094_2_0_9; -.
DR   OMA; NGWVLYK; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR004688; Ni/Co_transpt.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   PANTHER; PTHR31611; PTHR31611; 1.
DR   Pfam; PF03824; NicO; 1.
DR   TIGRFAMs; TIGR00802; nico; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..338
FT                   /note="Nickel transporter NixA"
FT                   /id="PRO_0000447264"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   338 AA;  37830 MW;  C5782CACB21765BA CRC64;
     MTVFKNERLS WLPYIAIVIL LHVIGFSFLW IAGKDHHILF GMGILAYTLG LRHAFDADHI
     AAIDNTVRKL LQQRKDPSGV GFYFSIGHSS VVFLMAVFLG VSVKWAKDEL PHFQDIGGTI
     GTLVSGFFLV LIGVLNLIIL ISLINLFAKL RREHIEEAEV DALLESRGLV SRFVGPYFKL
     ITRSWHVLPL GFLFGLGFDT ASEIALLALS SGASQQAISF IGILSLPILF ASGMSLLDTL
     DGVVMKYAYN WAFFNPIRKI YYNITITAIS VMAALVIGMI ELLQILADKL DLHGAFWAFI
     GSIEFDYLGY ILVALFLITW LISSLIWKFG RIEHKWSR