ID   NIX_AEDAE               Reviewed;         288 AA.
AC   A0A0F6MY85;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2015, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Male determiner protein Nix {ECO:0000303|PubMed:25999371};
GN   Name=Nix {ECO:0000303|PubMed:25999371};
OS   Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Aedini; Aedes; Stegomyia.
OX   NCBI_TaxID=7159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=25999371; DOI=10.1126/science.aaa2850;
RA   Hall A.B., Basu S., Jiang X., Qi Y., Timoshevskiy V.A., Biedler J.K.,
RA   Sharakhova M.V., Elahi R., Anderson M.A., Chen X.G., Sharakhov I.V.,
RA   Adelman Z.N., Tu Z.;
RT   "A male-determining factor in the mosquito Aedes aegypti.";
RL   Science 348:1268-1270(2015).
CC   -!- FUNCTION: Male determiner protein (M-factor) that controls male somatic
CC       sexual differentiation. Acts as a dominant factor that regulates the
CC       mRNA splicing of doublesex (dsx) or fruitless (fru) transcripts and
CC       promotes expression of male splice forms of dsx and fru.
CC       {ECO:0000303|PubMed:25999371}.
CC   -!- DEVELOPMENTAL STAGE: Male-specific expression. First detected 3 to 4
CC       hours after oviposition, corresponding to the beginning of the
CC       syncytial blastoderm stage before sex is established.
CC       {ECO:0000269|PubMed:25999371}.
CC   -!- DISRUPTION PHENOTYPE: Feminized males, which display features such as
CC       the absence of one or both gonocoxites and gonostyli, or antennae with
CC       fewer and shorter setae than normal males. Mosquitoes shifts the
CC       alternative splicing of dsx and fru toward their female isoforms.
CC       {ECO:0000269|PubMed:25999371}.
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DR   EMBL; KF732822; AHW46195.1; -; mRNA.
DR   AlphaFoldDB; A0A0F6MY85; -.
DR   SMR; A0A0F6MY85; -.
DR   VEuPathDB; VectorBase:AAEL022912; -.
DR   Proteomes; UP000008820; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0030238; P:male sex determination; IDA:UniProtKB.
DR   GO; GO:0046661; P:male sex differentiation; IDA:UniProtKB.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; IDA:UniProtKB.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 3.
DR   SUPFAM; SSF54928; SSF54928; 2.
DR   PROSITE; PS50102; RRM; 1.
PE   2: Evidence at transcript level;
KW   Differentiation; mRNA processing; mRNA splicing; Reference proteome;
KW   Repeat; RNA-binding; Sexual differentiation.
FT   CHAIN           1..288
FT                   /note="Male determiner protein Nix"
FT                   /id="PRO_0000441319"
FT   DOMAIN          19..94
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          108..179
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          205..282
FT                   /note="RRM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
SQ   SEQUENCE   288 AA;  33163 MW;  DBC83F3A863BE576 CRC64;
     MCKKRNAEIN AEFDIIKKYC IYIGNIPFFA SKNDVVVKFA EYGETCNIYM QSNKPHCDVK
     PAIVRYRSRK SVDKSLCLNN SKFGNTILIV LPLSLPYSRY LLTYDTCIVV YINDKNSCKT
     SMAELYDEFQ KIGDIQNMFK TTNNMIYINF ESEKSMQLSL ATKPFLINNN IFKIKKVERN
     INMCGLNSES QDFSTNLKKK LLYNRSIGIF GLPSNATEER IAQIFSRFGD IQKITLICDV
     VGNSKQYGFI YYKKRTSANA AKVIMDGENF EGNKISVRFV PEKKVFKN