NJMU_HUMAN
ID NJMU_HUMAN Reviewed; 396 AA.
AC Q9HAS0; Q7Z2H4;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein Njmu-R1;
GN Name=C17orf75;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Li J.M., Sha J.H., Zhou Z.M.;
RT "Njmu-R1: a novel new gene related to spermatogenesis.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [10]
RP INTERACTION WITH TBC1D23.
RX PubMed=29084197; DOI=10.1038/ncb3627;
RA Shin J.J.H., Gillingham A.K., Begum F., Chadwick J., Munro S.;
RT "TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at
RT the trans-Golgi.";
RL Nat. Cell Biol. 19:1424-1432(2017).
RN [11]
RP FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH FAM91A1
RP AND WDR11, AND INTERACTION WITH TBC1D23.
RX PubMed=29426865; DOI=10.1038/s41467-018-02919-4;
RA Navarro Negredo P., Edgar J.R., Manna P.T., Antrobus R., Robinson M.S.;
RT "The WDR11 complex facilitates the tethering of AP-1-derived vesicles.";
RL Nat. Commun. 9:596-596(2018).
CC -!- FUNCTION: As component of the WDR11 complex acts together with TBC1D23
CC to facilitate the golgin-mediated capture of vesicles generated using
CC AP-1 (PubMed:29426865). May have a role in spermatogenesis.
CC {ECO:0000269|PubMed:29426865}.
CC -!- SUBUNIT: Component of the complex WDR11 composed of C17orf75, FAM91A1
CC and WDR11; FAM91A1 and WDR11 are required for proper location of the
CC complex (PubMed:29426865). Interacts with TBC1D23; this interaction may
CC be indirect and recruits TBC1D23 to AP-1-derived vesicles
CC (PubMed:29084197, PubMed:29426865). {ECO:0000269|PubMed:29084197,
CC ECO:0000269|PubMed:29426865}.
CC -!- INTERACTION:
CC Q9HAS0; Q9UNS2: COPS3; NbExp=3; IntAct=EBI-12954949, EBI-350590;
CC Q9HAS0; Q68J44: DUSP29; NbExp=3; IntAct=EBI-12954949, EBI-1054321;
CC Q9HAS0; Q14696: MESD; NbExp=3; IntAct=EBI-12954949, EBI-6165891;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000269|PubMed:29426865}. Cytoplasmic vesicle
CC {ECO:0000269|PubMed:29426865}.
CC -!- TISSUE SPECIFICITY: Highly expressed in testis and also expressed in
CC fetal testis.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG23214.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF305686; AAG23214.1; ALT_FRAME; mRNA.
DR EMBL; BC026017; AAH26017.1; -; mRNA.
DR EMBL; BC035715; AAH35715.1; -; mRNA.
DR EMBL; BC047325; AAH47325.1; -; mRNA.
DR EMBL; BC054035; AAH54035.1; -; mRNA.
DR CCDS; CCDS58537.1; -.
DR RefSeq; NP_071739.2; NM_022344.3.
DR AlphaFoldDB; Q9HAS0; -.
DR BioGRID; 122087; 53.
DR IntAct; Q9HAS0; 14.
DR STRING; 9606.ENSP00000464275; -.
DR GlyGen; Q9HAS0; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9HAS0; -.
DR PhosphoSitePlus; Q9HAS0; -.
DR BioMuta; C17orf75; -.
DR DMDM; 114152850; -.
DR EPD; Q9HAS0; -.
DR jPOST; Q9HAS0; -.
DR MassIVE; Q9HAS0; -.
DR MaxQB; Q9HAS0; -.
DR PaxDb; Q9HAS0; -.
DR PeptideAtlas; Q9HAS0; -.
DR PRIDE; Q9HAS0; -.
DR ProteomicsDB; 81423; -.
DR Antibodypedia; 1366; 191 antibodies from 22 providers.
DR DNASU; 64149; -.
DR Ensembl; ENST00000577809.6; ENSP00000464275.1; ENSG00000108666.10.
DR GeneID; 64149; -.
DR KEGG; hsa:64149; -.
DR MANE-Select; ENST00000577809.6; ENSP00000464275.1; NM_022344.4; NP_071739.2.
DR UCSC; uc002hhg.4; human.
DR CTD; 64149; -.
DR GeneCards; C17orf75; -.
DR HGNC; HGNC:30173; C17orf75.
DR HPA; ENSG00000108666; Low tissue specificity.
DR neXtProt; NX_Q9HAS0; -.
DR OpenTargets; ENSG00000108666; -.
DR PharmGKB; PA142672223; -.
DR VEuPathDB; HostDB:ENSG00000108666; -.
DR eggNOG; ENOG502QRCR; Eukaryota.
DR GeneTree; ENSGT00390000005481; -.
DR InParanoid; Q9HAS0; -.
DR OMA; LFEGMGN; -.
DR OrthoDB; 1351924at2759; -.
DR PhylomeDB; Q9HAS0; -.
DR TreeFam; TF330763; -.
DR PathwayCommons; Q9HAS0; -.
DR SignaLink; Q9HAS0; -.
DR BioGRID-ORCS; 64149; 26 hits in 1055 CRISPR screens.
DR ChiTaRS; C17orf75; human.
DR GenomeRNAi; 64149; -.
DR Pharos; Q9HAS0; Tbio.
DR PRO; PR:Q9HAS0; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q9HAS0; protein.
DR Bgee; ENSG00000108666; Expressed in right testis and 167 other tissues.
DR ExpressionAtlas; Q9HAS0; baseline and differential.
DR Genevisible; Q9HAS0; HS.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:UniProtKB.
DR GO; GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; IDA:UniProtKB.
DR GO; GO:0099041; P:vesicle tethering to Golgi; IDA:UniProtKB.
DR InterPro; IPR028280; Njmu-R1.
DR PANTHER; PTHR14416; PTHR14416; 1.
DR Pfam; PF15053; Njmu-R1; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Golgi apparatus; Phosphoprotein; Reference proteome.
FT CHAIN 1..396
FT /note="Protein Njmu-R1"
FT /id="PRO_0000096865"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 8
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT CONFLICT 314
FT /note="L -> F (in Ref. 1; AAG23214)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 44622 MW; 7789DF3685170DB0 CRC64;
MLPSLQESMD GDEKELESSE EGGSAEERRL EPPSSSHYCL YSYRGSRLAQ QRGDSEDGSP
SGTNAETPSG DDFSLSLADT NLPSEVEPEL RSFIAKRLSR GAVFEGLGNV ASVELKIPGY
RVGCYYCLFQ NEKLLPETVT IDSERNPSEY VVCFLGGSEK GLELFRLELD KYIQGLKNNM
NCEARGLESH IKSYLSSWFE DVVCPIQRVV LLFQEKLTFL LHAALSYTPV EVKESDEKTK
RDINRFLSVA SLQGLIHEGT MTSLCMAMTE EQHKSVVIDC SSSQPQFCNA GSNRFCEDWM
QAFLNGAKGG NPFLFRQVLE NFKLKAIQDT NNLKRFIRQA EMNHYALFKC YMFLKNCGSG
DILLKIVKVE HEEMPEAKNV IAVLEEFMKE ALDQSF
