NKAI1_HUMAN
ID NKAI1_HUMAN Reviewed; 207 AA.
AC Q4KMZ8; A2VDJ3; B7Z5F5; B7Z5W9; Q9H9M7;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 1;
DE Short=Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein 1;
DE AltName: Full=Protein FAM77C;
DE Flags: Precursor;
GN Name=NKAIN1; Synonyms=FAM77C;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-207 (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Interacts with ATP1B1 C-terminus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q4KMZ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4KMZ8-3; Sequence=VSP_056113, VSP_056114;
CC -!- SIMILARITY: Belongs to the NKAIN family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH96707.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH98173.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH98249.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAI31519.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB14196.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAX07629.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AK022712; BAB14196.1; ALT_INIT; mRNA.
DR EMBL; AK298874; BAH12891.1; -; mRNA.
DR EMBL; AK299507; BAH13055.1; -; mRNA.
DR EMBL; AC114495; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471059; EAX07629.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC096707; AAH96707.2; ALT_INIT; mRNA.
DR EMBL; BC098173; AAH98173.2; ALT_INIT; mRNA.
DR EMBL; BC098249; AAH98249.2; ALT_INIT; mRNA.
DR EMBL; BC131518; AAI31519.1; ALT_INIT; mRNA.
DR CCDS; CCDS339.2; -. [Q4KMZ8-1]
DR RefSeq; NP_078798.2; NM_024522.2. [Q4KMZ8-1]
DR AlphaFoldDB; Q4KMZ8; -.
DR BioGRID; 122717; 122.
DR STRING; 9606.ENSP00000362841; -.
DR TCDB; 8.A.118.1.2; the na+k+-atpase beta-subunit interacting nkain (nkain) family.
DR GlyGen; Q4KMZ8; 1 site.
DR iPTMnet; Q4KMZ8; -.
DR PhosphoSitePlus; Q4KMZ8; -.
DR BioMuta; NKAIN1; -.
DR DMDM; 341942232; -.
DR MassIVE; Q4KMZ8; -.
DR PaxDb; Q4KMZ8; -.
DR PeptideAtlas; Q4KMZ8; -.
DR PRIDE; Q4KMZ8; -.
DR ProteomicsDB; 62209; -. [Q4KMZ8-1]
DR ProteomicsDB; 6688; -.
DR Antibodypedia; 16738; 80 antibodies from 16 providers.
DR DNASU; 79570; -.
DR Ensembl; ENST00000373736.7; ENSP00000362841.2; ENSG00000084628.10. [Q4KMZ8-1]
DR GeneID; 79570; -.
DR KEGG; hsa:79570; -.
DR MANE-Select; ENST00000373736.7; ENSP00000362841.2; NM_024522.3; NP_078798.2.
DR UCSC; uc010ogd.3; human. [Q4KMZ8-1]
DR CTD; 79570; -.
DR DisGeNET; 79570; -.
DR GeneCards; NKAIN1; -.
DR HGNC; HGNC:25743; NKAIN1.
DR HPA; ENSG00000084628; Group enriched (adrenal gland, brain, skeletal muscle, tongue).
DR MIM; 612871; gene.
DR neXtProt; NX_Q4KMZ8; -.
DR OpenTargets; ENSG00000084628; -.
DR VEuPathDB; HostDB:ENSG00000084628; -.
DR eggNOG; KOG4556; Eukaryota.
DR GeneTree; ENSGT00940000159949; -.
DR InParanoid; Q4KMZ8; -.
DR OMA; KYLIMYA; -.
DR OrthoDB; 1281511at2759; -.
DR PhylomeDB; Q4KMZ8; -.
DR TreeFam; TF321348; -.
DR PathwayCommons; Q4KMZ8; -.
DR BioGRID-ORCS; 79570; 17 hits in 1067 CRISPR screens.
DR ChiTaRS; NKAIN1; human.
DR GenomeRNAi; 79570; -.
DR Pharos; Q4KMZ8; Tdark.
DR PRO; PR:Q4KMZ8; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q4KMZ8; protein.
DR Bgee; ENSG00000084628; Expressed in cortical plate and 106 other tissues.
DR ExpressionAtlas; Q4KMZ8; baseline and differential.
DR Genevisible; Q4KMZ8; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051117; F:ATPase binding; IEA:Ensembl.
DR GO; GO:0002028; P:regulation of sodium ion transport; IBA:GO_Central.
DR InterPro; IPR008516; Na/K-Atpase_Interacting.
DR PANTHER; PTHR13084; PTHR13084; 1.
DR Pfam; PF05640; NKAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..207
FT /note="Sodium/potassium-transporting ATPase subunit beta-1-
FT interacting protein 1"
FT /id="PRO_0000412169"
FT TOPO_DOM 22..34
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..146
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056113"
FT VAR_SEQ 65..91
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056114"
FT CONFLICT 88
FT /note="Q -> R (in Ref. 1; BAH13055)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 207 AA; 23552 MW; 5BDC3771B3E24E3E CRC64;
MGKCSGRCTL VAFCCLQLVA ALERQIFDFL GYQWAPILAN FLHIMAVILG IFGTVQYRSR
YLILYAAWLV LWVGWNAFII CFYLEVGQLS QDRDFIMTFN TSLHRSWWME NGPGCLVTPV
LNSRLALEDH HVISVTGCLL DYPYIEALSS ALQIFLALFG FVFACYVSKV FLEEEDSFDF
IGGFDSYGYQ APQKTSHLQL QPLYTSG
