NKAI2_MOUSE
ID NKAI2_MOUSE Reviewed; 208 AA.
AC Q4PNJ2; A6MHP5; A6MHP6; A6MHP7; B2RUC0; Q4PNJ3; Q5BLW8;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 2;
DE Short=Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein 2;
DE AltName: Full=T-cell lymphoma breakpoint-associated target protein 1;
GN Name=Nkain2; Synonyms=Tcba1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/NJ; TISSUE=Brain;
RX PubMed=16145689; DOI=10.1002/humu.20235;
RA Bocciardi R., Giorda R., Marigo V., Zordan P., Montanaro D., Gimelli S.,
RA Seri M., Lerone M., Ravazzolo R., Gimelli G.;
RT "Molecular characterization of a t(2;6) balanced translocation that is
RT associated with a complex phenotype and leads to truncation of the TCBA1
RT gene.";
RL Hum. Mutat. 26:426-436(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=15908570; DOI=10.1136/jmg.2004.029660;
RA Yue Y., Stout K., Grossmann B., Zechner U., Brinckmann A., White C.,
RA Pilz D.T., Haaf T.;
RT "Disruption of TCBA1 associated with a de novo t(1;6)(q32.2;q22.3)
RT presenting in a child with developmental delay and recurrent infections.";
RL J. Med. Genet. 43:143-147(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INTERACTION WITH ATP1B1,
RP AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=17606467; DOI=10.1093/hmg/ddm167;
RA Gorokhova S., Bibert S., Geering K., Heintz N.;
RT "A novel family of transmembrane proteins interacting with beta subunits of
RT the Na,K-ATPase.";
RL Hum. Mol. Genet. 16:2394-2410(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Interacts with ATP1B1. {ECO:0000269|PubMed:17606467}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Transcript variant 1;
CC IsoId=Q4PNJ2-1; Sequence=Displayed;
CC Name=2; Synonyms=Transcript variant 3;
CC IsoId=Q4PNJ2-2; Sequence=VSP_021903;
CC Name=3; Synonyms=Transcript variant 2;
CC IsoId=Q4PNJ2-3; Sequence=VSP_029299;
CC -!- TISSUE SPECIFICITY: Detected in the brain only and specifically in
CC neurons; expressed in multiple regions such as cerebral cortex,
CC thalamus, cerebellum, olfactory bulb and brainstem, but not in the
CC hippocampus. {ECO:0000269|PubMed:17606467}.
CC -!- SIMILARITY: Belongs to the NKAIN family. {ECO:0000305}.
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DR EMBL; DQ065606; AAY68415.1; -; mRNA.
DR EMBL; DQ065607; AAY68416.1; -; mRNA.
DR EMBL; AY940478; AAX33293.1; -; mRNA.
DR EMBL; EF058049; ABN51167.1; -; mRNA.
DR EMBL; EF058050; ABN51168.1; -; mRNA.
DR EMBL; EF058051; ABN51169.1; -; mRNA.
DR EMBL; BC141063; AAI41064.1; -; mRNA.
DR CCDS; CCDS78805.1; -. [Q4PNJ2-1]
DR CCDS; CCDS87984.1; -. [Q4PNJ2-2]
DR RefSeq; NP_001013429.2; NM_001013411.2. [Q4PNJ2-1]
DR RefSeq; NP_001020457.2; NM_001025286.2. [Q4PNJ2-2]
DR AlphaFoldDB; Q4PNJ2; -.
DR SMR; Q4PNJ2; -.
DR STRING; 10090.ENSMUSP00000140463; -.
DR PhosphoSitePlus; Q4PNJ2; -.
DR PRIDE; Q4PNJ2; -.
DR ProteomicsDB; 293659; -. [Q4PNJ2-1]
DR Antibodypedia; 51965; 66 antibodies from 15 providers.
DR Ensembl; ENSMUST00000191234; ENSMUSP00000140463; ENSMUSG00000069670. [Q4PNJ2-1]
DR Ensembl; ENSMUST00000219125; ENSMUSP00000151959; ENSMUSG00000069670. [Q4PNJ2-2]
DR GeneID; 432450; -.
DR KEGG; mmu:432450; -.
DR UCSC; uc007etw.2; mouse. [Q4PNJ2-1]
DR UCSC; uc007ety.2; mouse. [Q4PNJ2-2]
DR CTD; 154215; -.
DR MGI; MGI:1923447; Nkain2.
DR VEuPathDB; HostDB:ENSMUSG00000069670; -.
DR eggNOG; KOG4556; Eukaryota.
DR GeneTree; ENSGT00940000160565; -.
DR HOGENOM; CLU_090781_0_0_1; -.
DR InParanoid; Q4PNJ2; -.
DR OMA; AWLVIWM; -.
DR PhylomeDB; Q4PNJ2; -.
DR BioGRID-ORCS; 432450; 0 hits in 65 CRISPR screens.
DR ChiTaRS; Nkain2; mouse.
DR PRO; PR:Q4PNJ2; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q4PNJ2; protein.
DR Bgee; ENSMUSG00000069670; Expressed in sciatic nerve and 115 other tissues.
DR ExpressionAtlas; Q4PNJ2; baseline and differential.
DR Genevisible; Q4PNJ2; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0002028; P:regulation of sodium ion transport; IBA:GO_Central.
DR InterPro; IPR008516; Na/K-Atpase_Interacting.
DR PANTHER; PTHR13084; PTHR13084; 1.
DR Pfam; PF05640; NKAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..208
FT /note="Sodium/potassium-transporting ATPase subunit beta-1-
FT interacting protein 2"
FT /id="PRO_0000265089"
FT TRANSMEM 1..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 159..208
FT /note="LAGFIYACYVVRCITEEEDSFDFIGGFDSYGYQGPQKTSHLQLQPMYMSK
FT -> VSVDNKPLLLVFLELFFLGKHCVQKDCMLMWNVCGHFF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16145689, ECO:0000303|PubMed:17606467"
FT /id="VSP_021903"
FT VAR_SEQ 206..208
FT /note="MSK -> IKFPWITTDSKFYISASSDMLNCKRRSK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17606467"
FT /id="VSP_029299"
FT CONFLICT 73
FT /note="V -> G (in Ref. 1; AAY68415/AAY68416)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="N -> Y (in Ref. 2; AAX33293)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="S -> P (in Ref. 2; AAX33293)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 208 AA; 23895 MW; 54EFBB877B43B54F CRC64;
MGYCSGRCTL IFICGMQLVC VLERQIFDFL GYQWAPILAN FVHIIIVILG LFGTIQYRPR
YVTGYAVWLV LWVTWNVFVI CFYLEAGDLS KETDLILTFN ISMHRSWWME NGPGCMVTSV
TPAPDWAPED HRYITVSGCF LDYQYIEVAH SSLQIVLALA GFIYACYVVR CITEEEDSFD
FIGGFDSYGY QGPQKTSHLQ LQPMYMSK
