NKAI3_MOUSE
ID NKAI3_MOUSE Reviewed; 181 AA.
AC Q3URJ8; A2ANL4; A6MHP8; A6MHQ0; Q6DI91; Q8BPL2; Q8C7P6;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 3;
DE Short=Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein 3;
DE AltName: Full=Protein FAM77D;
GN Name=Nkain3; Synonyms=Fam77d;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=17606467; DOI=10.1093/hmg/ddm167;
RA Gorokhova S., Bibert S., Geering K., Heintz N.;
RT "A novel family of transmembrane proteins interacting with beta subunits of
RT the Na,K-ATPase.";
RL Hum. Mol. Genet. 16:2394-2410(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Eye, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Interacts with ATP1B1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Transcript variant 1;
CC IsoId=Q3URJ8-1; Sequence=Displayed;
CC Name=2; Synonyms=Transcript variant 2;
CC IsoId=Q3URJ8-2; Sequence=VSP_021858;
CC Name=3; Synonyms=Transcript variant 3;
CC IsoId=Q3URJ8-3; Sequence=VSP_029300;
CC -!- TISSUE SPECIFICITY: Detected in the brain only and specifically in
CC neurons. Expressed in multiple regions such as cerebral cortex,
CC thalamus, hippocampus, olfactory bulb and brainstem as well as in
CC cerebellum with low expression in granular cell layer.
CC {ECO:0000269|PubMed:17606467}.
CC -!- SIMILARITY: Belongs to the NKAIN family. {ECO:0000305}.
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DR EMBL; EF058052; ABN51170.1; -; mRNA.
DR EMBL; EF058053; ABN51171.1; -; mRNA.
DR EMBL; EF058054; ABN51172.1; -; mRNA.
DR EMBL; AK049776; BAC33916.1; -; mRNA.
DR EMBL; AK053823; BAC35541.1; -; mRNA.
DR EMBL; AK141456; BAE24690.1; -; mRNA.
DR EMBL; AL831710; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL928932; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX649549; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC075683; AAH75683.1; -; mRNA.
DR CCDS; CCDS17997.1; -. [Q3URJ8-2]
DR CCDS; CCDS71349.1; -. [Q3URJ8-1]
DR RefSeq; NP_001277339.1; NM_001290410.1. [Q3URJ8-1]
DR RefSeq; NP_766575.2; NM_172987.2. [Q3URJ8-2]
DR AlphaFoldDB; Q3URJ8; -.
DR STRING; 10090.ENSMUSP00000100063; -.
DR iPTMnet; Q3URJ8; -.
DR PhosphoSitePlus; Q3URJ8; -.
DR PaxDb; Q3URJ8; -.
DR PRIDE; Q3URJ8; -.
DR ProteomicsDB; 293662; -. [Q3URJ8-1]
DR ProteomicsDB; 293663; -. [Q3URJ8-2]
DR ProteomicsDB; 293664; -. [Q3URJ8-3]
DR Antibodypedia; 63542; 10 antibodies from 6 providers.
DR DNASU; 269513; -.
DR Ensembl; ENSMUST00000102998; ENSMUSP00000100063; ENSMUSG00000055761. [Q3URJ8-2]
DR Ensembl; ENSMUST00000119374; ENSMUSP00000113113; ENSMUSG00000055761. [Q3URJ8-1]
DR GeneID; 269513; -.
DR KEGG; mmu:269513; -.
DR UCSC; uc008scl.1; mouse. [Q3URJ8-1]
DR UCSC; uc008scm.1; mouse. [Q3URJ8-2]
DR UCSC; uc008scn.1; mouse. [Q3URJ8-3]
DR CTD; 286183; -.
DR MGI; MGI:2444830; Nkain3.
DR VEuPathDB; HostDB:ENSMUSG00000055761; -.
DR eggNOG; KOG4556; Eukaryota.
DR GeneTree; ENSGT00940000161484; -.
DR HOGENOM; CLU_090781_0_1_1; -.
DR InParanoid; Q3URJ8; -.
DR OMA; TALWITW; -.
DR OrthoDB; 1281511at2759; -.
DR PhylomeDB; Q3URJ8; -.
DR TreeFam; TF321348; -.
DR BioGRID-ORCS; 269513; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Nkain3; mouse.
DR PRO; PR:Q3URJ8; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q3URJ8; protein.
DR Bgee; ENSMUSG00000055761; Expressed in dorsal root ganglion and 46 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0002028; P:regulation of sodium ion transport; IBA:GO_Central.
DR InterPro; IPR008516; Na/K-Atpase_Interacting.
DR PANTHER; PTHR13084; PTHR13084; 1.
DR Pfam; PF05640; NKAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..181
FT /note="Sodium/potassium-transporting ATPase subunit beta-1-
FT interacting protein 3"
FT /id="PRO_0000263094"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 158..181
FT /note="LVGFVYACYVISISMEEEDTCRNK -> VRRTKTGLL (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_021858"
FT VAR_SEQ 158..181
FT /note="LVGFVYACYVISISMEEEDTCRNK -> VSHTFESLSDSLLC (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:17606467"
FT /id="VSP_029300"
FT CONFLICT 12
FT /note="C -> Y (in Ref. 2; BAC33916)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="E -> G (in Ref. 2; BAC35541)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 181 AA; 20700 MW; F241D3A72F787F91 CRC64;
MGCCTGRCSL VCLCALQLLS ALERQIFDFL GFQWAPILGN FLHIIVVILG LFGTIQYRPR
YIMVYTVWTA LWVTWNVFII CFYLEVGGLS KDTDLMTFNI SVHRSWWREH GPGCVRRVLP
PSAHGMMDDY TYVSVTGCVV DFQYLEVIHS AVQILLSLVG FVYACYVISI SMEEEDTCRN
K
