NKAI4_MOUSE
ID NKAI4_MOUSE Reviewed; 208 AA.
AC Q9JMG4; A2AWN3; A6MHQ2; A6MHQ3; Q5EBQ3; Q9D1V9; Q9D8W0;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4;
DE Short=Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein 4;
DE AltName: Full=Protein FAM77A;
GN Name=Nkain4; Synonyms=Fam77a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10679242; DOI=10.1006/bbrc.2000.2170;
RA Inoue S., Sano H., Ohta M.;
RT "Growth suppression of Escherichia coli by induction of expression of
RT mammalian genes with transmembrane or ATPase domains.";
RL Biochem. Biophys. Res. Commun. 268:553-561(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INTERACTION WITH ATP1B1,
RP AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=17606467; DOI=10.1093/hmg/ddm167;
RA Gorokhova S., Bibert S., Geering K., Heintz N.;
RT "A novel family of transmembrane proteins interacting with beta subunits of
RT the Na,K-ATPase.";
RL Hum. Mol. Genet. 16:2394-2410(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Corpus striatum, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain, Heart, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Interacts with ATP1B1. {ECO:0000269|PubMed:17606467}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Transcript variant 1;
CC IsoId=Q9JMG4-1; Sequence=Displayed;
CC Name=2; Synonyms=Transcript variant 2;
CC IsoId=Q9JMG4-2; Sequence=VSP_029302;
CC Name=3; Synonyms=Transcript variant 3;
CC IsoId=Q9JMG4-3; Sequence=VSP_029301;
CC -!- TISSUE SPECIFICITY: Ubiquitous. Expressed in multiple regions of the
CC brain such as cerebral cortex, thalamus, hippocampus, olfactory bulb
CC and brainstem as well as in cerebellum with low expression in granular
CC cell layer. {ECO:0000269|PubMed:17606467}.
CC -!- SIMILARITY: Belongs to the NKAIN family. {ECO:0000305}.
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DR EMBL; AB030182; BAA92746.1; -; mRNA.
DR EMBL; EF058055; ABN51173.1; -; mRNA.
DR EMBL; EF058056; ABN51174.1; -; mRNA.
DR EMBL; EF058057; ABN51175.1; -; mRNA.
DR EMBL; AK005262; BAB23914.1; -; mRNA.
DR EMBL; AK007632; BAB25149.1; -; mRNA.
DR EMBL; AK021096; BAB32298.1; -; mRNA.
DR EMBL; AL954707; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX649560; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC061107; AAH61107.1; -; mRNA.
DR EMBL; BC089330; AAH89330.1; -; mRNA.
DR CCDS; CCDS17189.1; -. [Q9JMG4-1]
DR CCDS; CCDS50841.1; -. [Q9JMG4-2]
DR RefSeq; NP_001135405.1; NM_001141933.1. [Q9JMG4-2]
DR RefSeq; NP_067401.3; NM_021426.4. [Q9JMG4-1]
DR AlphaFoldDB; Q9JMG4; -.
DR STRING; 10090.ENSMUSP00000099342; -.
DR PhosphoSitePlus; Q9JMG4; -.
DR PaxDb; Q9JMG4; -.
DR PRIDE; Q9JMG4; -.
DR ProteomicsDB; 252897; -. [Q9JMG4-1]
DR ProteomicsDB; 252898; -. [Q9JMG4-2]
DR ProteomicsDB; 252899; -. [Q9JMG4-3]
DR Antibodypedia; 29667; 123 antibodies from 19 providers.
DR Ensembl; ENSMUST00000103053; ENSMUSP00000099342; ENSMUSG00000027574. [Q9JMG4-1]
DR Ensembl; ENSMUST00000108873; ENSMUSP00000104501; ENSMUSG00000027574. [Q9JMG4-2]
DR GeneID; 58237; -.
DR KEGG; mmu:58237; -.
DR UCSC; uc008okf.2; mouse. [Q9JMG4-1]
DR UCSC; uc008okg.2; mouse. [Q9JMG4-2]
DR UCSC; uc008okh.2; mouse. [Q9JMG4-3]
DR CTD; 128414; -.
DR MGI; MGI:1915372; Nkain4.
DR VEuPathDB; HostDB:ENSMUSG00000027574; -.
DR eggNOG; KOG4556; Eukaryota.
DR GeneTree; ENSGT00940000157989; -.
DR HOGENOM; CLU_090781_0_0_1; -.
DR InParanoid; Q9JMG4; -.
DR OMA; FMDYQYI; -.
DR OrthoDB; 1281511at2759; -.
DR PhylomeDB; Q9JMG4; -.
DR TreeFam; TF321348; -.
DR BioGRID-ORCS; 58237; 1 hit in 40 CRISPR screens.
DR ChiTaRS; Nkain4; mouse.
DR PRO; PR:Q9JMG4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9JMG4; protein.
DR Bgee; ENSMUSG00000027574; Expressed in dorsal pancreas and 169 other tissues.
DR ExpressionAtlas; Q9JMG4; baseline and differential.
DR Genevisible; Q9JMG4; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0002028; P:regulation of sodium ion transport; IBA:GO_Central.
DR InterPro; IPR008516; Na/K-Atpase_Interacting.
DR PANTHER; PTHR13084; PTHR13084; 1.
DR Pfam; PF05640; NKAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..208
FT /note="Sodium/potassium-transporting ATPase subunit beta-1-
FT interacting protein 4"
FT /id="PRO_0000079438"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 158..208
FT /note="LLGFVYGCYVVSVLTEEEDSFDFIGGFDPFPLYHVNEKPSSLLSKQAYLPA
FT -> VSVGSGLFYLGQSRGFSFTQRNQISSLLSIIASMTCKE (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17606467"
FT /id="VSP_029301"
FT VAR_SEQ 178..208
FT /note="FDFIGGFDPFPLYHVNEKPSSLLSKQAYLPA -> CLHK (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:17606467"
FT /id="VSP_029302"
FT CONFLICT 15
FT /note="A -> R (in Ref. 3; BAB25149)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="S -> R (in Ref. 3; BAB32298)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 208 AA; 23042 MW; A143A84733B4B83A CRC64;
MGFCSGRCTL LALCALQLVT ALERQVFDFL GYQWAPILAN FTHIIVVILG LFGTIQYRPR
YIVVYVVWAA VWVTWNVFII CFYLEVGGLS KDSELLTFNL SGHRSWWEEH GPGCLHEEEA
TAGLGALHGQ SLVVGAGCAM VHSYVEALHS GLQILLALLG FVYGCYVVSV LTEEEDSFDF
IGGFDPFPLY HVNEKPSSLL SKQAYLPA
