NKAP1_HUMAN
ID NKAP1_HUMAN Reviewed; 292 AA.
AC Q6ZUT1; Q5RL41; Q6IN53; Q7Z357; Q8N2T3;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Uncharacterized protein NKAPD1;
DE AltName: Full=NKAP domain containing protein 1 {ECO:0000312|HGNC:HGNC:25569};
GN Name=NKAPD1 {ECO:0000312|HGNC:HGNC:25569}; Synonyms=C11orf57;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Colon endothelium;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, Cervix, and PNS;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-123; SER-125 AND SER-126
RP (ISOFORM 2), PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-94; SER-96 AND
RP SER-97 (ISOFORM 3), AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [5]
RP SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-8 AND LYS-76, AND IDENTIFICATION
RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=28112733; DOI=10.1038/nsmb.3366;
RA Hendriks I.A., Lyon D., Young C., Jensen L.J., Vertegaal A.C.,
RA Nielsen M.L.;
RT "Site-specific mapping of the human SUMO proteome reveals co-modification
RT with phosphorylation.";
RL Nat. Struct. Mol. Biol. 24:325-336(2017).
CC -!- INTERACTION:
CC Q6ZUT1; P12814: ACTN1; NbExp=3; IntAct=EBI-3920396, EBI-351710;
CC Q6ZUT1; P35609: ACTN2; NbExp=3; IntAct=EBI-3920396, EBI-77797;
CC Q6ZUT1; Q08043: ACTN3; NbExp=3; IntAct=EBI-3920396, EBI-2880652;
CC Q6ZUT1; Q9NRZ7: AGPAT3; NbExp=3; IntAct=EBI-3920396, EBI-2803601;
CC Q6ZUT1; Q9BXS5: AP1M1; NbExp=3; IntAct=EBI-3920396, EBI-541426;
CC Q6ZUT1; Q96CW1: AP2M1; NbExp=3; IntAct=EBI-3920396, EBI-297683;
CC Q6ZUT1; A0A0C4DG62: ARL6IP4; NbExp=3; IntAct=EBI-3920396, EBI-12248874;
CC Q6ZUT1; Q8N7W2-2: BEND7; NbExp=6; IntAct=EBI-3920396, EBI-10181188;
CC Q6ZUT1; Q9BWC9: CCDC106; NbExp=5; IntAct=EBI-3920396, EBI-711501;
CC Q6ZUT1; Q9UK58: CCNL1; NbExp=6; IntAct=EBI-3920396, EBI-2836773;
CC Q6ZUT1; Q53HL2: CDCA8; NbExp=3; IntAct=EBI-3920396, EBI-979174;
CC Q6ZUT1; Q8NHQ1: CEP70; NbExp=6; IntAct=EBI-3920396, EBI-739624;
CC Q6ZUT1; P38432: COIL; NbExp=3; IntAct=EBI-3920396, EBI-945751;
CC Q6ZUT1; Q5HYN5: CT45A1; NbExp=3; IntAct=EBI-3920396, EBI-12051833;
CC Q6ZUT1; Q8IYF1: ELOA2; NbExp=3; IntAct=EBI-3920396, EBI-741705;
CC Q6ZUT1; Q9NQT4: EXOSC5; NbExp=3; IntAct=EBI-3920396, EBI-371876;
CC Q6ZUT1; Q8N9E0: FAM133A; NbExp=3; IntAct=EBI-3920396, EBI-10268158;
CC Q6ZUT1; Q96NE9-2: FRMD6; NbExp=3; IntAct=EBI-3920396, EBI-13213391;
CC Q6ZUT1; O95995: GAS8; NbExp=3; IntAct=EBI-3920396, EBI-1052570;
CC Q6ZUT1; U3KQK0: H2BC15; NbExp=3; IntAct=EBI-3920396, EBI-12142839;
CC Q6ZUT1; A0A024R8L2: hCG_1987119; NbExp=3; IntAct=EBI-3920396, EBI-14103818;
CC Q6ZUT1; Q9UGU5: HMGXB4; NbExp=3; IntAct=EBI-3920396, EBI-7261162;
CC Q6ZUT1; Q9NPI7: KRCC1; NbExp=3; IntAct=EBI-3920396, EBI-2805442;
CC Q6ZUT1; O75367: MACROH2A1; NbExp=3; IntAct=EBI-3920396, EBI-2868511;
CC Q6ZUT1; Q96EZ8: MCRS1; NbExp=6; IntAct=EBI-3920396, EBI-348259;
CC Q6ZUT1; Q9BU76: MMTAG2; NbExp=3; IntAct=EBI-3920396, EBI-742459;
CC Q6ZUT1; Q6ZUT1: NKAPD1; NbExp=3; IntAct=EBI-3920396, EBI-3920396;
CC Q6ZUT1; Q5M9Q1: NKAPL; NbExp=3; IntAct=EBI-3920396, EBI-11423380;
CC Q6ZUT1; Q9UBU9: NXF1; NbExp=3; IntAct=EBI-3920396, EBI-398874;
CC Q6ZUT1; O43395: PRPF3; NbExp=3; IntAct=EBI-3920396, EBI-744322;
CC Q6ZUT1; O75400-2: PRPF40A; NbExp=3; IntAct=EBI-3920396, EBI-5280197;
CC Q6ZUT1; Q14498: RBM39; NbExp=6; IntAct=EBI-3920396, EBI-395290;
CC Q6ZUT1; Q2KHN1: RNF151; NbExp=6; IntAct=EBI-3920396, EBI-12002474;
CC Q6ZUT1; Q15287: RNPS1; NbExp=3; IntAct=EBI-3920396, EBI-395959;
CC Q6ZUT1; Q8TA86: RP9; NbExp=3; IntAct=EBI-3920396, EBI-630339;
CC Q6ZUT1; P62851: RPS25; NbExp=3; IntAct=EBI-3920396, EBI-353054;
CC Q6ZUT1; O00560: SDCBP; NbExp=8; IntAct=EBI-3920396, EBI-727004;
CC Q6ZUT1; Q9H190: SDCBP2; NbExp=6; IntAct=EBI-3920396, EBI-742426;
CC Q6ZUT1; Q9BV90: SNRNP25; NbExp=3; IntAct=EBI-3920396, EBI-9675976;
CC Q6ZUT1; Q8N9Q2: SREK1IP1; NbExp=6; IntAct=EBI-3920396, EBI-10268630;
CC Q6ZUT1; Q96MF2: STAC3; NbExp=6; IntAct=EBI-3920396, EBI-745680;
CC Q6ZUT1; Q15573: TAF1A; NbExp=3; IntAct=EBI-3920396, EBI-2510647;
CC Q6ZUT1; Q9NVV9: THAP1; NbExp=6; IntAct=EBI-3920396, EBI-741515;
CC Q6ZUT1; P40222: TXLNA; NbExp=3; IntAct=EBI-3920396, EBI-359793;
CC Q6ZUT1; P26368-2: U2AF2; NbExp=3; IntAct=EBI-3920396, EBI-11097439;
CC Q6ZUT1; Q96PQ6: ZNF317; NbExp=7; IntAct=EBI-3920396, EBI-1210473;
CC Q6ZUT1; P51814-6: ZNF41; NbExp=3; IntAct=EBI-3920396, EBI-12700258;
CC Q6ZUT1-2; Q8N7W2-2: BEND7; NbExp=3; IntAct=EBI-10180231, EBI-10181188;
CC Q6ZUT1-2; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-10180231, EBI-739624;
CC Q6ZUT1-2; Q14498: RBM39; NbExp=3; IntAct=EBI-10180231, EBI-395290;
CC Q6ZUT1-2; O00560: SDCBP; NbExp=3; IntAct=EBI-10180231, EBI-727004;
CC Q6ZUT1-2; Q9H190: SDCBP2; NbExp=3; IntAct=EBI-10180231, EBI-742426;
CC Q6ZUT1-2; Q96MF2: STAC3; NbExp=3; IntAct=EBI-10180231, EBI-745680;
CC Q6ZUT1-2; Q15573: TAF1A; NbExp=3; IntAct=EBI-10180231, EBI-2510647;
CC Q6ZUT1-2; Q9NVV9: THAP1; NbExp=3; IntAct=EBI-10180231, EBI-741515;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6ZUT1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6ZUT1-2; Sequence=VSP_022686;
CC Name=3;
CC IsoId=Q6ZUT1-3; Sequence=VSP_022685, VSP_022686;
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DR EMBL; AK125340; BAC86137.1; -; mRNA.
DR EMBL; BX538107; CAD98020.1; -; mRNA.
DR EMBL; BC005403; AAH05403.3; -; mRNA.
DR EMBL; BC030546; AAH30546.3; -; mRNA.
DR EMBL; BC048133; AAH48133.2; -; mRNA.
DR EMBL; BC072455; AAH72455.2; -; mRNA.
DR CCDS; CCDS41715.1; -. [Q6ZUT1-1]
DR CCDS; CCDS73383.1; -. [Q6ZUT1-3]
DR CCDS; CCDS8356.2; -. [Q6ZUT1-2]
DR RefSeq; NP_001076439.1; NM_001082970.1. [Q6ZUT1-1]
DR RefSeq; NP_001287946.1; NM_001301017.1. [Q6ZUT1-1]
DR RefSeq; NP_001287948.1; NM_001301019.1. [Q6ZUT1-3]
DR RefSeq; NP_001287950.1; NM_001301021.1.
DR AlphaFoldDB; Q6ZUT1; -.
DR BioGRID; 120512; 151.
DR IntAct; Q6ZUT1; 124.
DR MINT; Q6ZUT1; -.
DR STRING; 9606.ENSP00000376767; -.
DR iPTMnet; Q6ZUT1; -.
DR PhosphoSitePlus; Q6ZUT1; -.
DR BioMuta; NKAPD1; -.
DR DMDM; 125863638; -.
DR EPD; Q6ZUT1; -.
DR jPOST; Q6ZUT1; -.
DR MassIVE; Q6ZUT1; -.
DR MaxQB; Q6ZUT1; -.
DR PaxDb; Q6ZUT1; -.
DR PeptideAtlas; Q6ZUT1; -.
DR PRIDE; Q6ZUT1; -.
DR ProteomicsDB; 68355; -. [Q6ZUT1-1]
DR ProteomicsDB; 68356; -. [Q6ZUT1-2]
DR ProteomicsDB; 68357; -. [Q6ZUT1-3]
DR Antibodypedia; 45617; 95 antibodies from 16 providers.
DR DNASU; 55216; -.
DR Ensembl; ENST00000280352.13; ENSP00000339076.7; ENSG00000150776.18. [Q6ZUT1-1]
DR Ensembl; ENST00000393047.8; ENSP00000376767.3; ENSG00000150776.18. [Q6ZUT1-2]
DR Ensembl; ENST00000420986.6; ENSP00000402208.2; ENSG00000150776.18. [Q6ZUT1-1]
DR Ensembl; ENST00000532163.5; ENSP00000432188.1; ENSG00000150776.18. [Q6ZUT1-3]
DR GeneID; 55216; -.
DR KEGG; hsa:55216; -.
DR MANE-Select; ENST00000393047.8; ENSP00000376767.3; NM_018195.4; NP_060665.3. [Q6ZUT1-2]
DR UCSC; uc001pmr.5; human. [Q6ZUT1-1]
DR CTD; 55216; -.
DR GeneCards; NKAPD1; -.
DR HGNC; HGNC:25569; NKAPD1.
DR HPA; ENSG00000150776; Low tissue specificity.
DR neXtProt; NX_Q6ZUT1; -.
DR OpenTargets; ENSG00000150776; -.
DR PharmGKB; PA143485352; -.
DR VEuPathDB; HostDB:ENSG00000150776; -.
DR eggNOG; ENOG502S0Y8; Eukaryota.
DR GeneTree; ENSGT00510000048628; -.
DR HOGENOM; CLU_082748_0_0_1; -.
DR InParanoid; Q6ZUT1; -.
DR OMA; KQTEETY; -.
DR PhylomeDB; Q6ZUT1; -.
DR TreeFam; TF332101; -.
DR PathwayCommons; Q6ZUT1; -.
DR SignaLink; Q6ZUT1; -.
DR BioGRID-ORCS; 55216; 334 hits in 1058 CRISPR screens.
DR ChiTaRS; C11orf57; human.
DR GenomeRNAi; 55216; -.
DR Pharos; Q6ZUT1; Tdark.
DR PRO; PR:Q6ZUT1; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q6ZUT1; protein.
DR Bgee; ENSG00000150776; Expressed in calcaneal tendon and 185 other tissues.
DR ExpressionAtlas; Q6ZUT1; baseline and differential.
DR Genevisible; Q6ZUT1; HS.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR InterPro; IPR043407; Nkap_D1.
DR PANTHER; PTHR46940; PTHR46940; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Isopeptide bond; Phosphoprotein; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..292
FT /note="Uncharacterized protein NKAPD1"
FT /id="PRO_0000274244"
FT REGION 47..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..171
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..195
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..214
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..249
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 271..285
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 8
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT CROSSLNK 76
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0007744|PubMed:28112733"
FT VAR_SEQ 1..29
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_022685"
FT VAR_SEQ 124
FT /note="D -> DS (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:17974005"
FT /id="VSP_022686"
FT CONFLICT 32
FT /note="K -> E (in Ref. 3; AAH48133)"
FT /evidence="ECO:0000305"
FT CONFLICT 96
FT /note="K -> R (in Ref. 2; CAD98020)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="D -> N (in Ref. 1; BAC86137)"
FT /evidence="ECO:0000305"
FT MOD_RES Q6ZUT1-2:123
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES Q6ZUT1-2:125
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES Q6ZUT1-2:126
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES Q6ZUT1-3:94
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES Q6ZUT1-3:96
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES Q6ZUT1-3:97
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
SQ SEQUENCE 292 AA; 34110 MW; 34053EE777795A10 CRC64;
MSRIPLGKVL LRNVIRHTDA HNKIQEESDM WKIRELEKQM EDAYRGTKRK MLPSSSSRMR
SDGFDEESQR YYWRPKNEIS GTLEDDFLKA KSWNKKFYDY EANMPDRWGH SGYKELYPEE
FETDSDQQDI TNGKKTSPQV KSSTHESRKH KKSKKSHKKK QKKRSHKKQK KSKKEATDIT
ADSSSEFSEE TGASGTRKGK QPHKRKKKSR KKSLKKPALF LEAESNTSHS DDSASSSSEE
SEERDTKKTK RKKREKKAHT SVANNEIQER TNKRTNWKVA TDERSAESSE DD
