NKAPL_MOUSE
ID NKAPL_MOUSE Reviewed; 395 AA.
AC Q5SZT7; Q3TV39; Q4KKZ9; Q5EBJ1; Q6P8T3; Q9CUJ2; Q9D5Y8;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=NKAP-like protein {ECO:0000250|UniProtKB:Q5M9Q1};
GN Name=Nkapl {ECO:0000312|MGI:MGI:1913957};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|Proteomes:UP000000589};
RN [1] {ECO:0000312|EMBL:BAE35781.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAE35781.1};
RC TISSUE=Testis {ECO:0000312|EMBL:BAE35781.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000312|EMBL:EDL32651.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:AAH99550.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAH99550.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-25 AND SER-149, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6] {ECO:0000305}
RP FUNCTION, INTERACTION WITH CIR1; HDAC3 AND RBPJ, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=25875095; DOI=10.1371/journal.pone.0124293;
RA Okuda H., Kiuchi H., Takao T., Miyagawa Y., Tsujimura A., Nonomura N.,
RA Miyata H., Okabe M., Ikawa M., Kawakami Y., Goshima N., Wada M., Tanaka H.;
RT "A novel transcriptional factor Nkapl is a germ cell-specific suppressor of
RT Notch signaling and is indispensable for spermatogenesis.";
RL PLoS ONE 10:E0124293-E0124293(2015).
CC -!- FUNCTION: Transcriptional repressor of Notch-mediated signaling.
CC Required for spermatogenesis. {ECO:0000269|PubMed:25875095}.
CC -!- SUBUNIT: Interacts with RBPJ, CIR1 and HDAC3.
CC {ECO:0000269|PubMed:25875095}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25875095}.
CC -!- TISSUE SPECIFICITY: Specific to testis (at protein level). Detected in
CC differenting spermatogonia and early spermatocytes (at protein level).
CC {ECO:0000269|PubMed:25875095}.
CC -!- DEVELOPMENTAL STAGE: Strongly expressed in testis from 3 weeks onwards.
CC {ECO:0000269|PubMed:25875095}.
CC -!- DISRUPTION PHENOTYPE: Viable. Males are infertile with reduced testis
CC size, while female fertility is not affected. In the testis, germ cell
CC development arrests at the pachytene spermatocyte stage leading to
CC complete absence of mature spermatozoa. {ECO:0000269|PubMed:25875095}.
CC -!- SIMILARITY: Belongs to the NKAP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH61080.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; AK014812; BAB29564.1; -; mRNA.
DR EMBL; AK015912; BAB30027.1; -; mRNA.
DR EMBL; AK160424; BAE35781.1; -; mRNA.
DR EMBL; CH466561; EDL32651.1; -; Genomic_DNA.
DR EMBL; AL589742; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC061080; AAH61080.1; ALT_SEQ; mRNA.
DR EMBL; BC089537; AAH89537.1; -; mRNA.
DR EMBL; BC099550; AAH99550.1; -; mRNA.
DR CCDS; CCDS26276.1; -.
DR RefSeq; NP_079995.2; NM_025719.3.
DR AlphaFoldDB; Q5SZT7; -.
DR SMR; Q5SZT7; -.
DR STRING; 10090.ENSMUSP00000065168; -.
DR iPTMnet; Q5SZT7; -.
DR PhosphoSitePlus; Q5SZT7; -.
DR jPOST; Q5SZT7; -.
DR MaxQB; Q5SZT7; -.
DR PaxDb; Q5SZT7; -.
DR PRIDE; Q5SZT7; -.
DR ProteomicsDB; 293665; -.
DR Antibodypedia; 44746; 81 antibodies from 14 providers.
DR DNASU; 66707; -.
DR Ensembl; ENSMUST00000068235; ENSMUSP00000065168; ENSMUSG00000059395.
DR GeneID; 66707; -.
DR KEGG; mmu:66707; -.
DR UCSC; uc007pqm.2; mouse.
DR CTD; 222698; -.
DR MGI; MGI:1913957; Nkapl.
DR VEuPathDB; HostDB:ENSMUSG00000059395; -.
DR eggNOG; KOG2812; Eukaryota.
DR GeneTree; ENSGT00940000163102; -.
DR HOGENOM; CLU_032439_1_0_1; -.
DR InParanoid; Q5SZT7; -.
DR OMA; GGYRYHH; -.
DR OrthoDB; 1561377at2759; -.
DR PhylomeDB; Q5SZT7; -.
DR TreeFam; TF315333; -.
DR BioGRID-ORCS; 66707; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q5SZT7; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q5SZT7; protein.
DR Bgee; ENSMUSG00000059395; Expressed in spermatocyte and 64 other tissues.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR040466; NKAP.
DR InterPro; IPR009269; NKAP_C.
DR PANTHER; PTHR13087; PTHR13087; 1.
DR Pfam; PF06047; Nkap_C; 1.
PE 1: Evidence at protein level;
KW Differentiation; Nucleus; Phosphoprotein; Reference proteome;
KW Spermatogenesis; Transcription; Transcription regulation.
FT CHAIN 1..395
FT /note="NKAP-like protein"
FT /id="PRO_0000437458"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 91..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..132
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..178
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 179..196
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..216
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..235
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 47
FT /note="G -> R (in Ref. 4; AAH99550/AAH89537/AAH61080)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="T -> K (in Ref. 4; AAH89537)"
FT /evidence="ECO:0000305"
FT CONFLICT 186
FT /note="R -> K (in Ref. 4; AAH89537)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="P -> K (in Ref. 4; AAH89537)"
FT /evidence="ECO:0000305"
FT CONFLICT 285..286
FT /note="KP -> NT (in Ref. 1; BAB29564)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 395 AA; 44894 MW; 48DA89F73ED62A1A CRC64;
MSPVSRSRPN EDTLGSQKRR RFSESPPSAL QTSRSPRGGG FGSLLSGPEG LQPPRGAQGV
RPLHPISRPR SGERPLPLGI RTLACSSSPI CGGYRYHHNY AGDPSWAEDQ EKEKEESYRQ
RRLKERERIG ELGAPEVWGL SPKIPEPDSD EHTPAEDEVK NQKSSSSDFT NEEKRTKTSH
STKKKRKKKP SKRKHKKYYD SSSDCHSSSD SDKKKVKTKK KEKKKKHRAK QLKKKRTKKE
YNDISCKASE RDLPEDAWME QSMSAESMDL IGPEAPVIHT SQDEKPLNYG HALLPGEGAA
MAEYVKAGKR IPRRGEIGLT SEEIASFECS GYVMSGSRHR RMEAVRLRKE NQIYSADEKR
ALASFNQEER RKRENKILAS FREMVYRKTK GKDDK
