ID   NKD_AEDAE               Reviewed;         715 AA.
AC   Q16HE8;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Protein naked cuticle homolog;
GN   Name=nkd {ECO:0000250|UniProtKB:Q9VVV9}; ORFNames=AAEL014048;
OS   Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Aedini; Aedes; Stegomyia.
OX   NCBI_TaxID=7159;
RN   [1] {ECO:0000312|EMBL:EAT33675.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LVPib12;
RX   PubMed=17510324; DOI=10.1126/science.1138878;
RA   Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA   Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA   Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA   Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA   Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA   Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA   El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA   Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA   Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA   Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA   Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA   Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA   Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA   Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA   Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA   Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA   Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT   "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL   Science 316:1718-1723(2007).
CC   -!- FUNCTION: Cell autonomous antagonist of the canonical Wnt signaling
CC       pathway. May activate a second Wnt signaling pathway that controls
CC       planar cell polarity. Required for neuroblast specification (By
CC       similarity). {ECO:0000250|UniProtKB:Q9VVV9}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9VVV9}.
CC       Cytoplasm {ECO:0000250|UniProtKB:Q9VVV9}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9VVV9}.
CC   -!- SIMILARITY: Belongs to the NKD family. {ECO:0000255}.
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DR   EMBL; CH478179; EAT33675.1; -; Genomic_DNA.
DR   RefSeq; XP_001664297.1; XM_001664247.1.
DR   AlphaFoldDB; Q16HE8; -.
DR   STRING; 7159.AAEL014048-PA; -.
DR   PRIDE; Q16HE8; -.
DR   VEuPathDB; VectorBase:AAEL027204; -.
DR   eggNOG; ENOG502QT1X; Eukaryota.
DR   HOGENOM; CLU_325475_0_0_1; -.
DR   InParanoid; Q16HE8; -.
DR   OMA; GNENCYE; -.
DR   PhylomeDB; Q16HE8; -.
DR   Proteomes; UP000008820; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR   GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IEA:UniProt.
DR   GO; GO:0014018; P:neuroblast fate specification; ISS:UniProtKB.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR040140; Nkd-like.
DR   PANTHER; PTHR22611; PTHR22611; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50222; EF_HAND_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cytoplasm; Developmental protein; Membrane; Metal-binding;
KW   Nucleus; Reference proteome; Wnt signaling pathway.
FT   CHAIN           1..715
FT                   /note="Protein naked cuticle homolog"
FT                   /id="PRO_0000312825"
FT   DOMAIN          18..53
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          256..282
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          305..334
FT                   /note="Required for nuclear localization and inhibition of
FT                   Wnt signaling"
FT                   /evidence="ECO:0000250|UniProtKB:Q9VVV9"
FT   REGION          639..690
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        640..663
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   715 AA;  78601 MW;  B3922F9CF252DC75 CRC64;
     MDLGIPEFTC DVSLEDGKKP QPLQFSFTLY DLDGHGKITK DDIAGIVSTI YESIGKSVVV
     PHYGSKTINV RLTVSPDGKT KQTASAVKKA AITPRRRYRP RKLISDDDGS DTSENCPRVM
     RTRANTVVAN TTINNNVKHK DNNVAKDEDV VDGLTKSSEA VETTFHNNLN GKGKTLNVKN
     DNIYESINNL KCCNIQAAQA SKPTVALSQS PGTLNNSTAT TVICRDCSLE GCTIDETLPL
     GTVVIPATTP AAAISSRAKR KVVRKSRSSR KASKLTDDFS RPRARSLSVG NENCYENMIG
     TTQEECWKSS LCRRELIEII RDSMVKNSLC FQPNRKPLEN SPKHRHRSHT IAARIGAEHC
     GETVMATQQA LVAAHETNLC GYDSYLHQTI CAAANANHAA LHLNGGVVGN GGVFTALPLT
     TSTPNRLLQH HSHHAHAKAK RKEHRLAVAT RSQVHHQVAQ PVKLSTAVLN QQYPNLSAEQ
     KLSRSINQVE KWLDNRSPKL VNKLKLAEEI AEKSPRVAAA KLKRSKSKEE ITHKSTKFEN
     VLTTDLLLDN LKITEDIAEL SVVTPKKIFN KECLISSATK KNIRTHHTTK TVSTTTAVVP
     PAVDKASKNL IQLQYASVPI NAELSECENL IRISDAEEEL HQSVQQQQGT HQQQQQPQSS
     VSSPTHHHHH HAGASLLGEN SGSSASAAST TAVHRYVHEH IHHHYHHFEN DPDES